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SSMEM1 (NM_145268) Human Tagged ORF Clone

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SKU
RC205412
C7orf45 (Myc-DDK-tagged)-Human chromosome 7 open reading frame 45 (C7orf45)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol SSMEM1
Synonyms C7orf45
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC205412 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGAGACCTTTTTTCCTTATTTTGGGAGGTAGATCCTCCCCCAATACCTGTAAATTGTGCCATTCCAA
ATCAGGATTATGAATGCTGGAAGGATGACTCTTGTGGAACCATAGGGAGCTTCCTGCTTTGGTATTTTGT
TATCGTATTTGTCCTGATGTTCTTCTCTAGGGCTTCTGTCTGGATGTCTGAGGATAAAAAGGATGAAGGC
AGTGGGACAAGTACTTCAGTAAGGAAAGCAAGCAAAGAGACTTCCTGTAAGCGGCAAAGCAAAGACAGTG
CCTGGGATCCCTCACAAACAATGAAGAAACCAAAGCAGAACCAACTTACCCCTGTAACCAACTCAGAAGT
GGCTTTGGTCAATGCCTATCCTGAACAAAGACGAGCCAGGCGCCAGTCTCAGTTCAATGAGGTGAACCAG
AACCAACATGACAGTGATACTACGGAGTATGGCAGTGAAGAGTCTAACTCAGAAGCCTCCTCGTGGAAGG
AGAGTGAAAGTGAACACCACCCATCACCAGACAGTATTAAGAGGAGAAAAATGGCTCAGAGGCAAAGGAA
TCTGGGAAGTTACCAAATGAGCGAAAGGCACTGCCTCCACTGCAAAGCCTTGAGAACCAACGAATGGTTG
GCGCACCATTCCCGACAGAAGCCTTCAGTAACACCGCCAATGAAAAGAGACAGTCAAGAGGAAAGTTCCA
TATCTGACATTAACAAGAAATTTAGTAAATTT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC205412 protein sequence
Red=Cloning site Green=Tags(s)

MGDLFSLFWEVDPPPIPVNCAIPNQDYECWKDDSCGTIGSFLLWYFVIVFVLMFFSRASVWMSEDKKDEG
SGTSTSVRKASKETSCKRQSKDSAWDPSQTMKKPKQNQLTPVTNSEVALVNAYPEQRRARRQSQFNEVNQ
NQHDSDTTEYGSEESNSEASSWKESESEHHPSPDSIKRRKMAQRQRNLGSYQMSERHCLHCKALRTNEWL
AHHSRQKPSVTPPMKRDSQEESSISDINKKFSKF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_145268
ORF Size 732 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_145268.4
RefSeq Size 1171 bp
RefSeq ORF 735 bp
Locus ID 136263
UniProt ID Q8WWF3, A4D1L0
Cytogenetics 7q32.2
Protein Families Transmembrane
MW 28.2 kDa
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"NM_145268" in other vectors (4)
SKU Description Size Price
RC205412L3 Lenti ORF clone of Human chromosome 7 open reading frame 45 (C7orf45), Myc-DDK-tagged 10 ug
$600.00
RC205412L4 Lenti ORF clone of Human chromosome 7 open reading frame 45 (C7orf45), mGFP tagged 10 ug
$600.00
RG205412 C7orf45 (tGFP-tagged) - Human chromosome 7 open reading frame 45 (C7orf45) 10 ug
$489.00 $500.00
SC306239 C7orf45 (untagged)-Human chromosome 7 open reading frame 45 (C7orf45) 10 ug
$330.00

Plasmid Map for RC205412

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC205412
Circular map for RC205412

file-alt Citations

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