AGFG1 (NM_004504) Human Tagged Lenti ORF Clone

  • LentiORF®
SKU
RC205375L2
Lenti ORF clone of Human ArfGAP with FG repeats 1 (AGFG1), transcript variant 2, mGFP tagged
  $823.00
3 Weeks*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol AGFG1
Synonyms HRB; RAB; RIP
Vector pLenti-C-mGFP
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection None
Sequence Data
ORF Nucleotide Sequence
The ORF insert of this clone is exactly the same as(RC205375).
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_004504
ORF Size 1686 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_004504.3
RefSeq Size 8684 bp
RefSeq ORF 1689 bp
Locus ID 3267
UniProt ID P52594
Cytogenetics 2q36.3
Domains ArfGap
MW 58.3 kDa
Summary The protein encoded by this gene is related to nucleoporins, a class of proteins that mediate nucleocytoplasmic transport. The encoded protein binds the activation domain of the human immunodeficiency virus Rev protein when Rev is assembled onto its RNA target, and is required for the nuclear export of Rev-directed RNAs. Multiple transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Sep 2008
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"NM_004504" in other vectors (6)
SKU Description Size Price
RC205375 AGFG1 (Myc-DDK-tagged)-Human ArfGAP with FG repeats 1 (AGFG1), transcript variant 2 10 ug
$523.00
RC205375L1 Lenti ORF clone of Human ArfGAP with FG repeats 1 (AGFG1), transcript variant 2, Myc-DDK-tagged 10 ug
$823.00
RC205375L3 Lenti ORF clone of Human ArfGAP with FG repeats 1 (AGFG1), transcript variant 2, Myc-DDK-tagged 10 ug
$823.00
RC205375L4 Lenti ORF clone of Human ArfGAP with FG repeats 1 (AGFG1), transcript variant 2, mGFP tagged 10 ug
$823.00
RG205375 AGFG1 (tGFP-tagged) - Human ArfGAP with FG repeats 1 (AGFG1), transcript variant 2 10 ug
$489.00 $723.00
SC117306 AGFG1 (untagged)-Human ArfGAP with FG repeats 1 (AGFG1), transcript variant 2 10 ug
$524.00

Plasmid Map for RC205375L2

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Cloning scheme for RC205375L2

Circular map for RC205375L2

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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