AGFG1 (NM_004504) Human Tagged Lenti ORF Clone
- LentiORF®
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | AGFG1 |
| Synonyms | HRB; RAB; RIP |
| Vector | pLenti-C-mGFP |
| E. coli Selection | Chloramphenicol (34 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_004504 |
| ORF Size | 1686 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_004504.3 |
| RefSeq Size | 8684 bp |
| RefSeq ORF | 1689 bp |
| Locus ID | 3267 |
| UniProt ID | P52594 |
| Cytogenetics | 2q36.3 |
| Domains | ArfGap |
| MW | 58.3 kDa |
| Summary | The protein encoded by this gene is related to nucleoporins, a class of proteins that mediate nucleocytoplasmic transport. The encoded protein binds the activation domain of the human immunodeficiency virus Rev protein when Rev is assembled onto its RNA target, and is required for the nuclear export of Rev-directed RNAs. Multiple transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Sep 2008 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_004504" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC205375 | AGFG1 (Myc-DDK-tagged)-Human ArfGAP with FG repeats 1 (AGFG1), transcript variant 2 | 10 ug |
$523.00
|
|
| RC205375L1 | Lenti ORF clone of Human ArfGAP with FG repeats 1 (AGFG1), transcript variant 2, Myc-DDK-tagged | 10 ug |
$823.00
|
|
| RC205375L3 | Lenti ORF clone of Human ArfGAP with FG repeats 1 (AGFG1), transcript variant 2, Myc-DDK-tagged | 10 ug |
$823.00
|
|
| RC205375L4 | Lenti ORF clone of Human ArfGAP with FG repeats 1 (AGFG1), transcript variant 2, mGFP tagged | 10 ug |
$823.00
|
|
| RG205375 | AGFG1 (tGFP-tagged) - Human ArfGAP with FG repeats 1 (AGFG1), transcript variant 2 | 10 ug |
$489.00
$723.00
|
|
| SC117306 | AGFG1 (untagged)-Human ArfGAP with FG repeats 1 (AGFG1), transcript variant 2 | 10 ug |
$524.00
|
|
Citations
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