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NAP1L3 (NM_004538) Human Tagged ORF Clone

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SKU
RC205297
NAP1L3 (Myc-DDK-tagged)-Human nucleosome assembly protein 1-like 3 (NAP1L3)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $471.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol NAP1L3
Synonyms MB20; NPL3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC205297 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAGAAGCAGATTTTAAAATGGTCTCGGAACCTGTCGCCCATGGGGTTGCCGAAGAGGAGATGGCTA
GCTCGACTAGTGATTCTGGGGAAGAATCTGACAGCAGTAGCTCTAGCAGCAGCACTAGTGACAGCAGCAG
CAGCAGCAGCACTAGTGGCAGCAGCAGCGGCAGCGGCAGCAGCAGCAGCAGCAGCGGCAGCACTAGCAGC
CGCAGCCGCTTGTATAGAAAGAAGAGGGTACCTGAGCCTTCCAGAAGGGCGCGGCGGGCCCCGTTGGGAA
CAAATTTCGTGGATAGGCTGCCTCAGGCAGTTAGAAATCGTGTGCAAGCGCTTAGAAACATTCAAGATGA
ATGTGACAAGGTAGATACCCTGTTCTTAAAAGCAATTCATGATCTTGAAAGAAAATATGCTGAACTCAAC
AAGCCTCTGTATGATAGGCGGTTTCAAATCATCAATGCAGAATACGAGCCTACAGAAGAAGAATGTGAAT
GGAATTCAGAGGATGAGGAGTTCAGCAGTGATGAGGAGGTGCAGGATAACACCCCTAGTGAAATGCCTCC
CTTAGAGGGTGAGGAAGAAGAAAACCCTAAAGAAAACCCAGAGGTGAAAGCTGAAGAGAAGGAAGTTCCT
AAAGAAATTCCTGAGGTGAAGGATGAAGAAAAGGAAGTTGCTAAAGAAATTACTGAGGTAAAGGCTGAAG
AAAAAGCAGATTCTAAAGACTGTATGGAGGCAACCCCTGAAGTAAAAGAAGATCCTAAAGAAGTCCCCCA
GGTAAAGGCAGATGATAAAGAACAGCCTAAAGCAACAGAGGCTAAGGCAAGGGCTGCAGTAAGAGAGACT
CATAAAAGAGTTCCTGAGGAAAGGCTTCAGGACAGTGTAGATCTTAAAAGAGCTAGGAAGGGAAAGCCTA
AAAGAGAAGACCCTAAAGGCATTCCTGACTATTGGCTGATTGTTTTAAAGAATGTTGACAAGCTCGGGCC
TATGATTCAGAAGTATGATGAGCCCATTCTGAAGTTCTTGTCGGATGTTAGCCTGAAGTTCTCAAAACCT
GGCCAGCCTGTAAGTTACACCTTTGAATTTCATTTTCTACCCAACCCATACTTCAGAAATGAGGTGCTGG
TGAAGACATATATAATAAAGGCAAAACCAGATCACAATGATCCCTTCTTTTCTTGGGGATGGGAAATTGA
AGATTGCAAAGGCTGCAAGATAGACTGGAGAAGAGGAAAAGATGTTACTGTGACAACTACCCAGAGTCGC
ACAACTGCTACTGGAGAAATTGAAATCCAGCCAAGAGTGGTTCCTAATGCATCATTCTTCAACTTCTTTA
GTCCTCCTGAGATTCCTATGATTGGGAAGCTGGAACCACGAGAAGATGCTATCCTGGATGAGGACTTTGA
AATTGGGCAGATTTTACATGATAATGTCATCCTGAAATCAATCTATTACTATACTGGAGAAGTCAATGGT
ACCTACTATCAATTTGGCAAACATTATGGAAACAAGAAATACAGAAAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC205297 protein sequence
Red=Cloning site Green=Tags(s)

MAEADFKMVSEPVAHGVAEEEMASSTSDSGEESDSSSSSSSTSDSSSSSSTSGSSSGSGSSSSSSGSTSS
RSRLYRKKRVPEPSRRARRAPLGTNFVDRLPQAVRNRVQALRNIQDECDKVDTLFLKAIHDLERKYAELN
KPLYDRRFQIINAEYEPTEEECEWNSEDEEFSSDEEVQDNTPSEMPPLEGEEEENPKENPEVKAEEKEVP
KEIPEVKDEEKEVAKEITEVKAEEKADSKDCMEATPEVKEDPKEVPQVKADDKEQPKATEAKARAAVRET
HKRVPEERLQDSVDLKRARKGKPKREDPKGIPDYWLIVLKNVDKLGPMIQKYDEPILKFLSDVSLKFSKP
GQPVSYTFEFHFLPNPYFRNEVLVKTYIIKAKPDHNDPFFSWGWEIEDCKGCKIDWRRGKDVTVTTTQSR
TTATGEIEIQPRVVPNASFFNFFSPPEIPMIGKLEPREDAILDEDFEIGQILHDNVILKSIYYYTGEVNG
TYYQFGKHYGNKKYRK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_004538
ORF Size 1518 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_004538.6
RefSeq Size 2761 bp
RefSeq ORF 1521 bp
Locus ID 4675
UniProt ID Q99457, Q8IYV1
Cytogenetics Xq21.32
Domains NAP
MW 57.6 kDa
Summary This gene is intronless and encodes a member of the nucleosome assembly protein (NAP) family. This gene is linked closely to a region of genes responsible for several X-linked cognitive disability syndromes. provided by RefSeq, Dec 2010
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Documents
Resources
"NM_004538" in other vectors (4)
SKU Description Size Price
RC205297L3 Lenti ORF clone of Human nucleosome assembly protein 1-like 3 (NAP1L3), Myc-DDK-tagged 10 ug
$771.00
RC205297L4 Lenti ORF clone of Human nucleosome assembly protein 1-like 3 (NAP1L3), mGFP tagged 10 ug
$771.00
RG205297 NAP1L3 (tGFP-tagged) - Human nucleosome assembly protein 1-like 3 (NAP1L3) 10 ug
$489.00 $671.00
SC117326 NAP1L3 (untagged)-Human nucleosome assembly protein 1-like 3 (NAP1L3) 10 ug
$472.00

Plasmid Map for RC205297

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC205297
Circular map for RC205297

file-alt Citations

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