FRAT1 (NM_005479) Human Tagged ORF Clone

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SKU
RC205287
FRAT1 (Myc-DDK-tagged)-Human frequently rearranged in advanced T-cell lymphomas (FRAT1)
  $300.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol FRAT1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC205287 representing NM_005479
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCGTGCCGGAGGGAGGAGGAAGAGGAAGCCGGCGAGGAGGCGGAGGGGGAGGAAGAGGAGGAGGACA
GCTTCCTCCTACTGCAGCAGTCAGTGGCGCTGGGCAGCTCGGGCGAGGTGGACCGGCTGGTGGCCCAGAT
CGGCGAGACGCTGCAGCTGGACGCGGCGCAGCACAGCCCGGCCTCGCCGTGCGGGCCCCCGGGGGCGCCG
CTGCGGGCCCCGGGGCCCCTGGCTGCGGCGGTGCCGGCGGACAAGGCCAGGTCCCCGGCGGTGCCGCTGC
TGCTGCCGCCCGCGTTGGCGGAGACTGTGGGCCCGGCGCCCCCTGGGGTCCTGCGCTGCGCCCTGGGGGA
CCGCGGCCGCGTGCGGGGCCGCGCTGCGCCCTACTGCGTGGCCGAGCTCGCCACAGGCCCCAGCGCGCTG
TCCCCACTGCCCCCTCAGGCCGACCTTGATGGGCCTCCGGGAGCTGGCAAGCAGGGCATCCCGCAGCCGC
TGTCGGGTCCGTGCCGGCGAGGATGGCTCCGGGGCGCCGCCGCCTCCCGCCGCCTGCAGCAGCGACGCGG
GTCCCAACCAGAAACCCGCACAGGCGACGACGACCCGCACCGGCTTCTGCAGCAGCTAGTGCTCTCTGGA
AACCTCATCAAGGAGGCCGTGCGAAGGCTTCATTCGCGACGGCTGCAGTTACGTGCAAAGCTTCCCCAAC
GCCCGCTCCTGGGACCTCTGTCGGCCCCGGTGCATGAACCCCCTTCGCCTCGCAGCCCTCGCGCGGCCTG
CAGTGACCCTGGCGCCTCCGGGAGGGCGCAGCTCAGAACTGGCGACGGCGTTCTTGTGCCTGGCAGC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC205287 representing NM_005479
Red=Cloning site Green=Tags(s)

MPCRREEEEEAGEEAEGEEEEEDSFLLLQQSVALGSSGEVDRLVAQIGETLQLDAAQHSPASPCGPPGAP
LRAPGPLAAAVPADKARSPAVPLLLPPALAETVGPAPPGVLRCALGDRGRVRGRAAPYCVAELATGPSAL
SPLPPQADLDGPPGAGKQGIPQPLSGPCRRGWLRGAAASRRLQQRRGSQPETRTGDDDPHRLLQQLVLSG
NLIKEAVRRLHSRRLQLRAKLPQRPLLGPLSAPVHEPPSPRSPRAACSDPGASGRAQLRTGDGVLVPGS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_005479
ORF Size 837 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_005479.4
RefSeq Size 2653 bp
RefSeq ORF 840 bp
Locus ID 10023
UniProt ID Q92837
Cytogenetics 10q24.1
Protein Families Druggable Genome, ES Cell Differentiation/IPS, Stem cell relevant signaling - Wnt Signaling pathway
Protein Pathways Wnt signaling pathway
MW 28.9 kDa
Summary The protein encoded by this gene belongs to the GSK-3-binding protein family. The protein inhibits GSK-3-mediated phosphorylation of beta-catenin and positively regulates the Wnt signaling pathway. It may function in tumor progression and in lymphomagenesis. provided by RefSeq, Oct 2008
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Documents
Resources
"NM_005479" in other vectors (4)
SKU Description Size Price
RC205287L3 Lenti ORF clone of Human frequently rearranged in advanced T-cell lymphomas (FRAT1), Myc-DDK-tagged 10 ug
$600.00
RC205287L4 Lenti ORF clone of Human frequently rearranged in advanced T-cell lymphomas (FRAT1), mGFP tagged 10 ug
$600.00
RG205287 FRAT1 (tGFP-tagged) - Human frequently rearranged in advanced T-cell lymphomas (FRAT1) 10 ug
$500.00
SC303653 FRAT1 (untagged)-Human frequently rearranged in advanced T-cell lymphomas (FRAT1) 10 ug
$300.00

Plasmid Map for RC205287

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Cloning scheme for RC205287

Circular map for RC205287

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