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KLHL11 (NM_018143) Human Tagged ORF Clone

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SKU
RC205228
KLHL11 (Myc-DDK-tagged)-Human kelch-like 11 (Drosophila) (KLHL11)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $648.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol KLHL11
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC205228 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGCTGCGGCAGTGGCGGCGGCGGCGGCGGCGGCCGCGGCTGCATCTCTTCAGGTACTGGAGATGG
AGAGCATGGAGACGGCCGCCGCCGGCTCGGCAGGACTGGCCGCCGAGGTCCGAGGCAGCGGCACGGTGGA
CTTCGGGCCTGGGCCGGGGATCTCTGCAATGGAGGCGAGCGGGGGCGATCCGGGCCCAGAAGCCGAGGAT
TTCGAGTGCAGCTCTCACTGCTCAGAGCTGTCCTGGCGGCAGAACGAGCAGCGGCGCCAGGGCCTCTTCT
GCGACATTACCCTGTGCTTCGGCGGGGCTGGAGGCCGCGAGTTCCGGGCCCACCGCTCGGTACTGGCTGC
CGCCACCGAGTACTTCACGCCCCTGCTCTCGGGCCAGTTTTCCGAGTCCCGCTCGGGACGGGTGGAGATG
CGCAAGTGGAGCTCCGAGCCGGGGCCCGAACCCGACACAGTGGAAGCCGTAATCGAGTACATGTACACCG
GGCGCATCCGCGTCAGCACGGGCAGCGTGCACGAGGTGCTGGAGTTGGCCGACAGGTTCCTACTCATTCG
TTTAAAAGAATTTTGTGGAGAATTTCTCAAGAAAAAACTTCATCTCTCAAATTGTGTGGCAATTCATAGC
TTAGCACACATGTACACCCTGAGCCAACTTGCTCTGAAGGCTGCTGATATGATACGGAGAAATTTCCACA
AAGTGATTCAGGATGAAGAATTTTATACGTTACCTTTCCATCTCATTAGAGACTGGCTTTCAGATTTGGA
AATTACTGTTGATTCTGAAGAGGTTCTCTTTGAAACCGTTTTGAAATGGGTTCAGAGAAATGCTGAAGAG
AGAGAGAGATACTTTGAAGAACTTTTTAAATTGCTCAGGTTGTCCCAGATGAAACCTACCTACCTTACTC
GACATGTCAAACCAGAGAGGCTGGTAGCCAATAATGAAGTTTGTGTCAAGTTGGTCGCTGACGCAGTGGA
GAGACATGCTCTGAGAGCTGAGAATATACAATCTGGCACATGCCAGCACCCCACTTCTCATGTGTCACTA
TTGCCTCGTTATGGGCAAAACATGGATGTGATCATGGTTATTGGAGGTGTGTCAGAAGGAGGGGACTATT
TAAGTGAATGTGTGGGATATTTTGTTGATGAGGACAGATGGGTAAATCTGCCACATATTCATAATCACCT
CGATGGACATGCTGTTGCAGTAACAGAATCCTACGTGTATGTTGCTGGATCAATGGAGCCAGGGTTTGCT
AAAACTGTAGAAAGGTATAACCCAAATTTGAATACATGGGAACATGTTTGTAGTCTGATGACAAGAAAGC
ATTCTTTTGGACTAACAGAAGTCAAAGGGAAGCTCTATAGCATTGGAGGACACGGCAACTTTAGTCCTGG
TTTTAAAGATGTGACTGTTTATAATCCTGAGCTTGATAAATGGCACAACTTGGAATCGGCACCAAAGATT
CTTCGAGATGTCAAAGCACTAGCCATTGAAGACCGGTTTGTATACATTGCCGCCCGCACTCCTGTAGACC
GGGACACTGAAGATGGATTAAAGGCTGTAATTACTTGCTATGATACAGAGACTCGACAGTGGCAAGATGT
GGAATCTTTGCCGCTTATTGACAATTACTGCTTTTTCCAAATGTCTGTGGTCAATTCAAACTTTTATCAG
ACAGCATCATGTTGTCCCAAGAGTTATTGTTTAGAAAACGAAGAGGCAGTAAGAAAAATTGCCAGCCAAG
TGTCTGATGAGATCCTTGAAAGCTTGCCTCCAGAAGTCCTAAGCATCGAAGGAGCAGCCATTTGCTATTA
CAAAGATGATGTCTTCATTATAGGAGGCTGGAAAAACAGTGATGATATTGATAAACAGTATCGGAAAGAA
GCCTACCGATATTGTGCGGAGAGGAAGAGGTGGATGCTTCTTCCTCCTATGCCACAACCTCGTTGTAGAG
CCACTGCTTGTCACGTGAGGATCCCGTACCGGTACTTGCATGGCACACAGAGATACCCTATGCCTCAAAA
CCTGATGTGGCAGAAGGACCGCATCAGACAGATGCAAGAGATACATCGTCACGCCCTGAACATGAGGCGA
GTGCCAAGCTCTCAGATTGAATGC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC205228 protein sequence
Red=Cloning site Green=Tags(s)

MAAAAVAAAAAAAAAASLQVLEMESMETAAAGSAGLAAEVRGSGTVDFGPGPGISAMEASGGDPGPEAED
FECSSHCSELSWRQNEQRRQGLFCDITLCFGGAGGREFRAHRSVLAAATEYFTPLLSGQFSESRSGRVEM
RKWSSEPGPEPDTVEAVIEYMYTGRIRVSTGSVHEVLELADRFLLIRLKEFCGEFLKKKLHLSNCVAIHS
LAHMYTLSQLALKAADMIRRNFHKVIQDEEFYTLPFHLIRDWLSDLEITVDSEEVLFETVLKWVQRNAEE
RERYFEELFKLLRLSQMKPTYLTRHVKPERLVANNEVCVKLVADAVERHALRAENIQSGTCQHPTSHVSL
LPRYGQNMDVIMVIGGVSEGGDYLSECVGYFVDEDRWVNLPHIHNHLDGHAVAVTESYVYVAGSMEPGFA
KTVERYNPNLNTWEHVCSLMTRKHSFGLTEVKGKLYSIGGHGNFSPGFKDVTVYNPELDKWHNLESAPKI
LRDVKALAIEDRFVYIAARTPVDRDTEDGLKAVITCYDTETRQWQDVESLPLIDNYCFFQMSVVNSNFYQ
TASCCPKSYCLENEEAVRKIASQVSDEILESLPPEVLSIEGAAICYYKDDVFIIGGWKNSDDIDKQYRKE
AYRYCAERKRWMLLPPMPQPRCRATACHVRIPYRYLHGTQRYPMPQNLMWQKDRIRQMQEIHRHALNMRR
VPSSQIEC

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_018143
ORF Size 2124 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_018143.3
RefSeq Size 2326 bp
RefSeq ORF 2127 bp
Locus ID 55175
UniProt ID Q9NVR0
Cytogenetics 17q21.2
Domains BTB, Kelch
Protein Families Secreted Protein
MW 80.1 kDa
Summary Component of a cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex that mediates the ubiquitination of target proteins, leading most often to their proteasomal degradation.UniProtKB/Swiss-Prot Function
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"NM_018143" in other vectors (4)
SKU Description Size Price
RC205228L3 Lenti ORF clone of Human kelch-like 11 (Drosophila) (KLHL11), Myc-DDK-tagged 10 ug
$948.00
RC205228L4 Lenti ORF clone of Human kelch-like 11 (Drosophila) (KLHL11), mGFP tagged 10 ug
$948.00
RG205228 KLHL11 (tGFP-tagged) - Human kelch-like 11 (Drosophila) (KLHL11) 10 ug
$789.00 $848.00
SC113703 KLHL11 (untagged)-Human kelch-like 11 (Drosophila) (KLHL11) 10 ug
$649.00

Plasmid Map for RC205228

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC205228
Circular map for RC205228

file-alt Citations

Opsoclonus-myoclonus syndrome associated with anti Kelch-like protein-11 antibodies in a young female patient without cancer
Fonseca, Varas, Godoy-Santín et al
J Neuroimmunol (2021) 355, 577570 DOI: 10.1016/j.jneuroim.2021.577570
Cited by 19
AI Summary: OriGene’s Myc-DDK-tagged KLHL11 human cDNA (#RC205228) was used to transfect HEK293 cells for the cell-based assay that enabled detection of Kelch-like protein-11 antibodies in this study.
Snippet: The detection of KLHL11-ab was performed by a cell-based assay, using human embryonic kidney cells (HEK293) previously transfected with Myc-DDK-tagged KLHL11 human complementary DNA (#RC205228;Origene, Rockville, MD) following standard procedures
Clinical significance of Kelch-like protein 11 antibodies
Maudes, Landa, Muñoz-Lopetegi et al
Neurol Neuroimmunol Neuroinflamm (2020) 7 (3) DOI: 10.1212/NXI.0000000000000666
AI Summary: OriGene’s Myc-DDK-tagged KLHL11 human cDNA (#RC205228) was used to transfect HEK293 cells for the cell-based assay that enabled detection of KLHL11 antibodies in patient samples in this study.
Snippet: …Specialty Sites: Equity, Diversity, and Inclusion;Innovations in Care Delivery;Without Free from Publisher;Transfection of Myc-DDK-tagged KLHL11 human complementary DNA (#RC205228;Origene, Rockville, MD) in HEK293 cells was performed following standard procedures. Transfected cells were fixed with 4% paraformaldehyde (PFA) for 15 minutes, blocked with 10% goat serum with 0.1% Triton for 1 hour, and sequentially incubated with the patient's sample (serum 1:10.000, CSF 1:1,000) and a commercial KLHL11-ab (1:1,000, #HPA054269;Sigma-Aldrich, St. …
Showing 1-2 of 2 papers.
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