SCML4 (NM_198081) Human Tagged ORF Clone

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SKU
RC205160
SCML4 (Myc-DDK-tagged)-Human sex comb on midleg-like 4 (Drosophila) (SCML4)
  $457.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol SCML4
Synonyms dJ47M23.1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC205160 representing NM_198081
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCAGTCTCAAAGGATCCCGGGGAGAAAGCGAGGCCGACCCTCACTTCACTCCACGCCTATGAAGATGG
CAGTTCATAACCTTTATTCTGCTTCAGCTGGCTCTTTACCAGCAGTGAAGATCCCAAAGAAAAGAGGGCG
GAAACCCGGGTACAAGATCAAGTCTCGGGTTCTCATGACTCCCTTAGCCCTCTCACCTCCGCGGAGTACC
CCAGAGCCCGACCTCAGCTCCATCCCTCAGGACGCAGCCACGGTCCCCAGCTTGGCGGCCCCACAGGCTC
TCACAGTCTGCCTCTACATCAACAAGCAGGCCAATGCGGGGCCCTATCTGGAGAGGAAGAAGGTGCAGCA
GCTCCCGGAGCATTTTGGGCCCGAGCGGCCATCGGCGGTGCTGCAGCAGGCCGTCCAAGCCTGCATCGAC
TGCGCCCACCAGCAGAAGCTGGTCTTCTCCCTGGTCAAGCAGGGCTATGGTGGTGAGATGGTGTCAGTCT
CGGCTTCCTTTGATGGCAAACAGCACCTGCGGAGCCTGCCTGTGGTGAACAGCATCGGCTATGTCCTCCG
CTTCCTCGCCAAGCTGTGCCGAAGCCTCCTGTGCGATGACCTCTTCAGCCACCAGCCCTTCCCCAGGGGC
TGCAGTGCCTCTGAGAAAGTCCAGGAGAAAGAGGAAGGGAGGATGGAATCAGTCAAGACAGTCACCACCG
AAGAGTACCTGGTGAACCCTGTGGGCATGAACCGCTACAGCGTGGACACCTCCGCCTCCACCTTTAACCA
CAGGGGCTCCTTGCACCCCTCCTCCTCGCTGTACTGCAAGAGGCAGAACTCTGGAGACAGCCACCTTGGG
GGTGGTCCTGCTGCCACCGCTGGTGGTCCCCGCACTAGCCCCATGTCTTCTGGTGGCCCCTCGGCACCTG
GGCTGAGGCCTCCAGCCTCCAGCCCCAAGAGAAACACGACCTCTCTTGAAGGAAACAGATGTGCCTCAAG
CCCTTCTCAGGATGCGCAGGATGCCAGGCGGCCACGGAGCAGGAACCCCTCCGCCTGGACTGTGGAGGAC
GTGGTGTGGTTTGTGAAGGACGCCGACCCACAGGCTCTGGGGCCTCACGTGGAGCTCTTCAGAAAGCACG
AGATTGATGGCAACGCTCTGCTGTTGCTGAAGAGTGACATGGTCATGAAGTACCTGGGCCTGAAGCTGGG
ACCTGCACTGAAACTCTGCTACCACATTGACAAACTGAAGCAAGCCAAGTTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC205160 representing NM_198081
Red=Cloning site Green=Tags(s)

MQSQRIPGRKRGRPSLHSTPMKMAVHNLYSASAGSLPAVKIPKKRGRKPGYKIKSRVLMTPLALSPPRST
PEPDLSSIPQDAATVPSLAAPQALTVCLYINKQANAGPYLERKKVQQLPEHFGPERPSAVLQQAVQACID
CAHQQKLVFSLVKQGYGGEMVSVSASFDGKQHLRSLPVVNSIGYVLRFLAKLCRSLLCDDLFSHQPFPRG
CSASEKVQEKEEGRMESVKTVTTEEYLVNPVGMNRYSVDTSASTFNHRGSLHPSSSLYCKRQNSGDSHLG
GGPAATAGGPRTSPMSSGGPSAPGLRPPASSPKRNTTSLEGNRCASSPSQDAQDARRPRSRNPSAWTVED
VVWFVKDADPQALGPHVELFRKHEIDGNALLLLKSDMVMKYLGLKLGPALKLCYHIDKLKQAKF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_198081
ORF Size 1242 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_198081.5
RefSeq Size 4540 bp
RefSeq ORF 1245 bp
Locus ID 256380
UniProt ID Q8N228, B4E0X3
Cytogenetics 6q21
MW 44.8 kDa
Summary Putative Polycomb group (PcG) protein. PcG proteins act by forming multiprotein complexes, which are required to maintain the transcriptionally repressive state of homeotic genes throughout development (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_198081" in other vectors (5)
SKU Description Size Price
RC205160L3 Lenti ORF clone of Human sex comb on midleg-like 4 (Drosophila) (SCML4), Myc-DDK-tagged 10 ug
$757.00
RC205160L4 Lenti ORF clone of Human sex comb on midleg-like 4 (Drosophila) (SCML4), mGFP tagged 10 ug
$757.00
RG205160 SCML4 (tGFP-tagged) - Human sex comb on midleg-like 4 (Drosophila) (SCML4) 10 ug
$657.00
SC123393 SCML4 (untagged)-Human sex comb on midleg-like 4 (Drosophila) (SCML4) 10 ug
$300.00
SC317662 SCML4 (untagged)-Human sex comb on midleg-like 4 (Drosophila) (SCML4) 10 ug
$457.00

Plasmid Map for RC205160

Download sequence for

Cloning scheme for RC205160

Circular map for RC205160

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