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MVB12B (NM_001011703) Human Tagged ORF Clone

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SKU
RC205093
MVB12B (Myc-DDK-tagged)-Human family with sequence similarity 125, member B (FAM125B), transcript variant 2
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol MVB12B
Synonyms C9orf28; FAM125B
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC205093 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGAAGCTGCTTCTGCGTGAGACGGAGCCGGGACCCGCCGCCGCCGCAGCCACCGCCGCCGCCGCCCC
AGCGGGGAACAGACCAGTCCACCATGCCTGAAGTCAAAGACCTCTCAGAAGCCTTGCCAGAAACATCAAT
GGATCCCATCACGGGAGTCGGGGTGGTGGCTTCTCGGAACCGAGCCCCGACAGGCTATGACGTAGTTGCA
CAGACAGCAGATGGTGTGGATGCTGACCTCTGGAAAGACGGCTTATTTAAATCCAAGGTTACCAGATACC
TGTGTTTCACAAGATCATTTTCCAAAGAAAATAGTCATCTGGGGAACGTGTTAGTAGATATGAAGCTCAT
TGACATCAAGGACACACTGCCTGTGGGCTTCATCCCAATTCAGGAGACGGTGGACACACAGGAAGTGGCT
TTTAGGAAGAAGAGGCTGTGCATTAAATTTATTCCACGGGATTCAACGGAAGCTGCGATTTGTGACATTC
GGATCATGGGCCGGACCAAGCAGGCCCCGCCTCAGTACACGTTTATTGGGGAACTGAACAGCATGGGGAT
CTGGTATCGAATGGGCAGAGTACCAAGAAATCATGACTCATCTCAACCCACAACGCCTTCCCAGTCATCA
GCTGCCTCCACCCCAGCCCCCAACCTTCCCAGG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC205093 protein sequence
Red=Cloning site Green=Tags(s)

MRSCFCVRRSRDPPPPQPPPPPPQRGTDQSTMPEVKDLSEALPETSMDPITGVGVVASRNRAPTGYDVVA
QTADGVDADLWKDGLFKSKVTRYLCFTRSFSKENSHLGNVLVDMKLIDIKDTLPVGFIPIQETVDTQEVA
FRKKRLCIKFIPRDSTEAAICDIRIMGRTKQAPPQYTFIGELNSMGIWYRMGRVPRNHDSSQPTTPSQSS
AASTPAPNLPR

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001011703
ORF Size 663 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001011703.3
RefSeq Size 2705 bp
RefSeq ORF 666 bp
Locus ID 89853
UniProt ID Q9H7P6
Cytogenetics 9q33.3
Protein Pathways Endocytosis
MW 24.5 kDa
Summary The protein encoded by this gene is a component of the ESCRT-I complex, a heterotetramer, which mediates the sorting of ubiquitinated cargo protein from the plasma membrane to the endosomal vesicle. ESCRT-I complex plays an essential role in HIV budding and endosomal protein sorting. Depletion and overexpression of this and related protein (MVB12A) inhibit HIV-1 infectivity and induce unusual viral assembly defects, indicating a role for MVB12 subunits in regulating ESCRT-mediated virus budding. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Aug 2011
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Documents
Resources
"NM_001011703" in other vectors (4)
SKU Description Size Price
RC205093L3 Lenti ORF clone of Human family with sequence similarity 125, member B (FAM125B), transcript variant 2, Myc-DDK-tagged 10 ug
$600.00
RC205093L4 Lenti ORF clone of Human family with sequence similarity 125, member B (FAM125B), transcript variant 2, mGFP tagged 10 ug
$600.00
RG205093 MVB12B (tGFP-tagged) - Human family with sequence similarity 125, member B (FAM125B), transcript variant 2 10 ug
$489.00 $500.00
SC301599 MVB12B (untagged)-Human family with sequence similarity 125, member B (FAM125B), transcript variant 2 10 ug
$330.00

Plasmid Map for RC205093

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC205093
Circular map for RC205093

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