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GLYATL2 (NM_145016) Human Tagged ORF Clone

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SKU
RC205043
GLYATL2 (Myc-DDK-tagged)-Human glycine-N-acyltransferase-like 2 (GLYATL2)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol GLYATL2
Synonyms BXMAS2-10; GATF-B
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC205043 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTTGTGCTTCATAACTCTCAGAAGCTGCAGATTCTGTATAAATCCTTAGAAAAGAGCATCCCTGAAT
CCATAAAGGTATATGGCGCCATTTTCAACATAAAAGATAAAAACCCTTTCAACATGGAGGTGCTGGTAGA
TGCCTGGCCAGATTACCAGATCGTCATTACCCGGCCTCAGAAACAGGAGATGAAAGATGACCAGGATCAT
TATACCAACACTTACCACATCTTCACCAAAGCTCCTGACAAATTAGAGGAAGTCCTGTCATACTCCAATG
TAATCAGCTGGGAGCAAACTTTGCAGATCCAAGGTTGCCAAGAGGGCTTGGATGAAGCAATAAGAAAGGT
TGCAACTTCAAAATCAGTGCAGGTAGATTACATGAAAACCATCCTCTTTATACCGGAATTACCAAAGAAA
CACAAGACCTCAAGTAATGACAAGATGGAGTTATTTGAAGTGGATGATGATAACAAGGAAGGAAACTTTT
CAAACATGTTCTTAGATGCTTCACATGCAGGTCTTGTGAATGAACACTGGGCCTTTGGGAAAAATGAGAG
GAGCTTGAAATATATTGAACGCTGCCTCCAGGATTTTCTAGGATTTGGTGTGCTGGGTCCAGAGGGCCAG
CTTGTCTCTTGGATTGTGATGGAACAGTCCTGTGAGTTGAGAATGGGTTATACTGTCCCCAAATACAGAC
ACCAAGGCAACATGTTGCAAATTGGTTATCATCTTGAAAAGTATCTTTCTCAGAAAGAAATCCCATTTTA
TTTCCATGTGGCAGATAATAATGAGAAAAGCCTACAGGCACTGAACAATTTGGGGTTTAAGATTTGTCCT
TGTGGCTGGCATCAGTGGAAATGCACCCCCAAGAAATATTGT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC205043 protein sequence
Red=Cloning site Green=Tags(s)

MLVLHNSQKLQILYKSLEKSIPESIKVYGAIFNIKDKNPFNMEVLVDAWPDYQIVITRPQKQEMKDDQDH
YTNTYHIFTKAPDKLEEVLSYSNVISWEQTLQIQGCQEGLDEAIRKVATSKSVQVDYMKTILFIPELPKK
HKTSSNDKMELFEVDDDNKEGNFSNMFLDASHAGLVNEHWAFGKNERSLKYIERCLQDFLGFGVLGPEGQ
LVSWIVMEQSCELRMGYTVPKYRHQGNMLQIGYHLEKYLSQKEIPFYFHVADNNEKSLQALNNLGFKICP
CGWHQWKCTPKKYC

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_145016
ORF Size 882 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_145016.4
RefSeq Size 1386 bp
RefSeq ORF 885 bp
Locus ID 219970
UniProt ID Q8WU03, A0A024R4Z5
Cytogenetics 11q12.1
MW 34.3 kDa
Summary Mitochondrial acyltransferase which transfers the acyl group to the N-terminus of glycine. Conjugates numerous substrates, such as arachidonoyl-CoA and saturated medium and long-chain acyl-CoAs ranging from chain-length C8:0-CoA to C18:0-CoA, to form a variety of N-acylglycines. Shows a preference for monounsaturated fatty acid oleoyl-CoA (C18:1-CoA) as an acyl donor. Does not exhibit any activity toward C22:6-CoA and chenodeoxycholoyl-CoA, nor toward serine or alanine.UniProtKB/Swiss-Prot Function
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"NM_145016" in other vectors (4)
SKU Description Size Price
RC205043L3 Lenti ORF clone of Human glycine-N-acyltransferase-like 2 (GLYATL2), Myc-DDK-tagged 10 ug
$600.00
RC205043L4 Lenti ORF clone of Human glycine-N-acyltransferase-like 2 (GLYATL2), mGFP tagged 10 ug
$600.00
RG205043 GLYATL2 (tGFP-tagged) - Human glycine-N-acyltransferase-like 2 (GLYATL2) 10 ug
$489.00 $500.00
SC123203 GLYATL2 (untagged)-Human glycine-N-acyltransferase-like 2 (GLYATL2) 10 ug
$300.00

Plasmid Map for RC205043

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC205043
Circular map for RC205043

file-alt Citations

Reversible lysine acetylation regulates activity of human glycine N-acyltransferase-like 2 (hGLYATL2): implications for production of glycine-conjugated signaling molecules
Waluk, Sucharski, Sipos et al
J. Biol. Chem. (2012) 287 (20), 16158-67 DOI: 10.1074/jbc.M112.347260
Cited by 1
AI Summary: OriGene’s HEK293 cell lysate overexpressing recombinant hGLYATL2 (C-terminal myc/DDK tag) was used in this study to analyze the role of lysine acetylation in regulating hGLYATL2 enzyme activity and its production of glycine-conjugated signaling molecules.
Snippet: A HEK293 cell lysate overexpressing recombinant hGLYATL2 containing a C-terminal myc/DDK tag was purchased (Origene Technologies, Rockville ... is involved in the detoxification of endogenous and exogenous xenobiotic acyl-CoAs and in the metabolism of short chain fatty
Showing 1-1 of 1 papers.
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