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RNF126 (NM_194460) Human Tagged ORF Clone

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SKU
RC204986
RNF126 (Myc-DDK-tagged)-Human ring finger protein 126 (RNF126)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

Frequently Bought Together (6)

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol RNF126
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC204986 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCGAGGCGTCGCCGCATCCCGGACGGTACTTCTGCCACTGCTGCTCCGTGGAGATCGTCCCGCGCC
TGCCGGATTATATCTGTCCAAGATGCGAGTCTGGTTTTATCGAGGAGCTTCCGGAAGAGACCAGGAGCAC
AGAAAATGGTTCTGCCCCCTCCACAGCTCCCACAGACCAGAGCCGGCCACCGCTGGAGCACGTGGACCAG
CACCTGTTCACGCTGCCGCAGGGCTACGGACAGTTTGCTTTCGGCATCTTCGATGACAGCTTCGAGATCC
CCACGTTCCCTCCTGGGGCGCAGGCTGACGACGGCAGGGACCCTGAGAGCCGGCGGGAGAGAGACCATCC
GTCCCGGCACCGGTACGGCGCCCGACAGCCCCGCGCCCGCCTCACCACGCGGCGGGCCACCGGCCGGCAC
GAAGGCGTCCCCACGCTGGAAGGGATCATCCAGCAGCTCGTCAACGGCATCATCACGCCCGCCACCATCC
CCAGCCTGGGCCCCTGGGGAGTCCTGCACTCAAACCCTATGGACTACGCCTGGGGGGCCAACGGCCTGGA
TGCCATCATCACACAGCTCCTCAATCAGTTTGAAAACACAGGCCCCCCACCGGCAGATAAAGAGAAAATC
CAGGCCCTCCCCACCGTCCCCGTCACTGAGGAGCACGTAGGCTCCGGGCTCGAGTGCCCTGTGTGCAAGG
ACGACTACGCGCTGGGTGAGCGTGTGCGGCAGCTGCCCTGCAACCACCTGTTCCACGACGGCTGCATCGT
GCCCTGGCTGGAGCAGCACGACAGCTGCCCCGTCTGCCGAAAAAGCCTCACGGGACAGAACACGGCCACG
AACCCCCCTGGCCTCACTGGGGTGAGCTTCTCCTCCTCGTCGTCATCGTCCTCCTCCAGCTCGCCCAGCA
ACGAGAACGCCACAAGCAACTCG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC204986 protein sequence
Red=Cloning site Green=Tags(s)

MAEASPHPGRYFCHCCSVEIVPRLPDYICPRCESGFIEELPEETRSTENGSAPSTAPTDQSRPPLEHVDQ
HLFTLPQGYGQFAFGIFDDSFEIPTFPPGAQADDGRDPESRRERDHPSRHRYGARQPRARLTTRRATGRH
EGVPTLEGIIQQLVNGIITPATIPSLGPWGVLHSNPMDYAWGANGLDAIITQLLNQFENTGPPPADKEKI
QALPTVPVTEEHVGSGLECPVCKDDYALGERVRQLPCNHLFHDGCIVPWLEQHDSCPVCRKSLTGQNTAT
NPPGLTGVSFSSSSSSSSSSSPSNENATSNS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_194460
ORF Size 933 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_194460.3
RefSeq Size 1665 bp
RefSeq ORF 936 bp
Locus ID 55658
UniProt ID Q9BV68, A0A024R206, A8K0Q1
Cytogenetics 19p13.3
Protein Families Druggable Genome
MW 33.9 kDa
Summary The protein encoded by this gene contains a RING finger domain, a motif present in a variety of functionally distinct proteins and known to be involved in protein-protein and protein-DNA interactions. provided by RefSeq, Jul 2008
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"NM_194460" in other vectors (6)
SKU Description Size Price
RC204986L1 Lenti ORF clone of Human ring finger protein 126 (RNF126), Myc-DDK-tagged 10 ug
$600.00
RC204986L2 Lenti ORF clone of Human ring finger protein 126 (RNF126), mGFP tagged 10 ug
$600.00
RC204986L3 Lenti ORF clone of Human ring finger protein 126 (RNF126), Myc-DDK-tagged 10 ug
$600.00
RC204986L4 Lenti ORF clone of Human ring finger protein 126 (RNF126), mGFP tagged 10 ug
$600.00
RG204986 RNF126 (tGFP-tagged) - Human ring finger protein 126 (RNF126) 10 ug
$489.00 $500.00
SC107741 RNF126 (untagged)-Human ring finger protein 126 (RNF126) 10 ug
$300.00

Plasmid Map for RC204986

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC204986
Circular map for RC204986

file-alt Citations

E3 Ligase RNF126 Directly Ubiquitinates Frataxin, Promoting Its Degradation: Identification of a Potential Therapeutic Target for Friedreich Ataxia
Benini, Fortuni, Condò et al
Cell Rep (2017) 18 (8), 2007-2017 DOI: 10.1016/j.celrep.2017.01.079
AI Summary: OriGene contributed to this study by providing plasmid vectors (pCMV6-RNF126, pCMV6-SYVN1, and pCMV6-FBXO44) used to express key E3 ligases for investigating the ubiquitination and degradation of frataxin.
Snippet: he plasmid vectors encoding for RNF126 (Myc-FLAG-tagged)-human ring finger protein 126 (pCMV6-RNF126), SYVN1 (Myc-FLAG-tagged)-human synovial apoptosis inhibitor 1 (pCMV6-SYVN1), and FBXO44 (Myc-FLAG-tagged)- human F-box protein 44 (pCMV6-FBXO44) were obtained from OriGene
Showing 1-1 of 1 papers.
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