RP9 (NM_203288) Human Tagged ORF Clone

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SKU
RC204944
RP9 (Myc-DDK-tagged)-Human retinitis pigmentosa 9 (autosomal dominant) (RP9)
  $300.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol RP9
Synonyms PAP-1; PAP1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC204944 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCGTCCCGGCCTGGGCGCGAGGACGTGGGGGCTGCGGGCGCGCGGCGGCCGCGTGAGCCGCCGGAGC
AGGAGCTGCAGCGACGTCGGGAGCAGAAGCGGCGGCGACACGACGCGCAGCAGCTGCAGCAGCTCAAGCA
CCTGGAGTCCTTTTACGAAAAACCTCCTCCTGGGCTTATCAAGGAAGATGAGACTAAGCCAGAAGATTGC
ATACCAGATGTACCAGGCAATGAACACGCCAGGGAATTTCTGGCTCATGCACCAACTAAAGGACTTTGGA
TGCCACTGGGGAAAGAAGTCAAAGTTATGCAGTGTTGGCGTTGCAAACGCTATGGTCACCGAACGGGTGA
CAAAGAATGCCCTTTCTTTATCAAAGGCAACCAAAAGTTAGAGCAGTTCAGAGTGGCACATGAAGATCCC
ATGTATGACATCATACGAGACAATAAACGACATGAAAAGGACGTAAGGATACAGCAGTTAAAACAGTTAC
TGGAGGATTCTACCTCAGATGAAGATAGGAGCAGCTCCAGTTCCTCTGAAGGTAAAGAGAAACACAAGAA
AAAGAAGAAGAAAGAAAAGCATAAGAAAAGGAAGAAAGAAAAGAAAAAGAAGAAAAAACGGAAGCACAAA
TCTTCCAAGTCAAATGAGGGTTCTGACTCAGAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC204944 protein sequence
Red=Cloning site Green=Tags(s)

MSSRPGREDVGAAGARRPREPPEQELQRRREQKRRRHDAQQLQQLKHLESFYEKPPPGLIKEDETKPEDC
IPDVPGNEHAREFLAHAPTKGLWMPLGKEVKVMQCWRCKRYGHRTGDKECPFFIKGNQKLEQFRVAHEDP
MYDIIRDNKRHEKDVRIQQLKQLLEDSTSDEDRSSSSSSEGKEKHKKKKKKEKHKKRKKEKKKKKKRKHK
SSKSNEGSDSE

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_203288
ORF Size 663 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_203288.2
RefSeq Size 1184 bp
RefSeq ORF 666 bp
Locus ID 6100
UniProt ID Q8TA86, A0A090N8Z0
Cytogenetics 7p14.3
MW 26.1 kDa
Summary The protein encoded by this gene can be bound and phosphorylated by the protooncogene PIM1 product, a serine/threonine protein kinase . This protein localizes in nuclear speckles containing the splicing factors, and has a role in pre-mRNA splicing. CBF1-interacting protein (CIR), a corepressor of CBF1, can also bind to this protein and effects alternative splicing. Mutations in this gene result in autosomal dominant retinitis pigmentosa-9. This gene has a pseudogene (GeneID: 441212), which is located in tandem array approximately 166 kb distal to this gene. provided by RefSeq, Sep 2009
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Documents
Resources
"NM_203288" in other vectors (5)
SKU Description Size Price
RC204944L3 Lenti ORF clone of Human retinitis pigmentosa 9 (autosomal dominant) (RP9), Myc-DDK-tagged 10 ug
$600.00
RC204944L4 Lenti ORF clone of Human retinitis pigmentosa 9 (autosomal dominant) (RP9), mGFP tagged 10 ug
$600.00
RG204944 RP9 (tGFP-tagged) - Human retinitis pigmentosa 9 (autosomal dominant) (RP9) 10 ug
$500.00
SC308100 RP9 (untagged)-Human retinitis pigmentosa 9 (autosomal dominant) (RP9) 10 ug
$330.00
SC321012 RP9 (untagged)-Human retinitis pigmentosa 9 (autosomal dominant) (RP9) 10 ug
$300.00

Plasmid Map for RC204944

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Cloning scheme for RC204944

Circular map for RC204944

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