TMIGD2 (NM_144615) Human Tagged ORF Clone

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SKU
RC204938
TMIGD2 (Myc-DDK-tagged)-Human transmembrane and immunoglobulin domain containing 2 (TMIGD2), transcript variant 1
  $300.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol TMIGD2
Synonyms CD28H; IGPR-1; IGPR1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC204938 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGTCCCCGGGCATGGTGCTGGGCCTCCTGGTGCAGATCTGGGCCCTGCAAGAAGCCTCAAGCCTGA
GCGTGCAGCAGGGGCCCAACTTGCTGCAGGTGAGGCAGGGCAGTCAGGCGACCCTGGTCTGCCAGGTGGA
CCAGGCCACAGCCTGGGAACGGCTCCGTGTTAAGTGGACAAAGGATGGGGCCATCCTGTGTCAACCGTAC
ATCACCAACGGCAGCCTCAGCCTGGGGGTCTGCGGGCCCCAGGGACGGCTCTCCTGGCAGGCACCCAGCC
ATCTCACCCTGCAGCTGGACCCTGTGAGCCTCAACCACAGCGGGGCGTACGTGTGCTGGGCGGCCGTAGA
GATTCCTGAGTTGGAGGAGGCTGAGGGCAACATAACAAGGCTCTTTGTGGACCCAGATGACCCCACACAG
AACAGAAACCGGATCGCAAGCTTCCCAGGATTCCTCTTCGTGCTGCTGGGGGTGGGAAGCATGGGTGTGG
CTGCGATCGTGTGGGGTGCCTGGTTCTGGGGCCGCCGCAGCTGCCAGCAAAGGGACTCAGGTAACAGCCC
AGGAAATGCATTCTACAGCAACGTCCTATACCGGCCCCGGGGGCCCCCAAAGAAGAGTGAGGACTGCTCT
GGAGAGGGGAAGGACCAGAGGGGCCAGAGCATTTATTCAACCTCCTTCCCGCAACCGGCCCCCCGCCAGC
CGCACCTGGCGTCAAGACCCTGCCCCAGCCCGAGACCCTGCCCCAGCCCCAGGCCCGGCCACCCCGTCTC
TATGGTCAGGGTCTCTCCTAGACCAAGCCCCACCCAGCAGCCGAGGCCAAAAGGGTTCCCCAAAGTGGGA
GAGGAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC204938 protein sequence
Red=Cloning site Green=Tags(s)

MGSPGMVLGLLVQIWALQEASSLSVQQGPNLLQVRQGSQATLVCQVDQATAWERLRVKWTKDGAILCQPY
ITNGSLSLGVCGPQGRLSWQAPSHLTLQLDPVSLNHSGAYVCWAAVEIPELEEAEGNITRLFVDPDDPTQ
NRNRIASFPGFLFVLLGVGSMGVAAIVWGAWFWGRRSCQQRDSGNSPGNAFYSNVLYRPRGPPKKSEDCS
GEGKDQRGQSIYSTSFPQPAPRQPHLASRPCPSPRPCPSPRPGHPVSMVRVSPRPSPTQQPRPKGFPKVG
EE

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_144615
ORF Size 846 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_144615.1, NP_653216.1
RefSeq Size 1282 bp
RefSeq ORF 849 bp
Locus ID 126259
UniProt ID Q96BF3
Cytogenetics 19p13.3
Protein Families Transmembrane
MW 30.7 kDa
Summary Plays a role in cell-cell interaction, cell migration, and angiogenesis. Through interaction with HHLA2, costimulates T-cells in the context of TCR-mediated activation. Enhances T-cell proliferation and cytokine production via an AKT-dependent signaling cascade.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_144615" in other vectors (4)
SKU Description Size Price
RC204938L3 Lenti ORF clone of Human transmembrane and immunoglobulin domain containing 2 (TMIGD2), transcript variant 1, Myc-DDK-tagged 10 ug
$600.00
RC204938L4 Lenti ORF clone of Human transmembrane and immunoglobulin domain containing 2 (TMIGD2), transcript variant 1, mGFP tagged 10 ug
$600.00
RG204938 TMIGD2 (tGFP-tagged) - Human transmembrane and immunoglobulin domain containing 2 (TMIGD2), transcript variant 1 10 ug
$489.00 $500.00
SC123174 TMIGD2 (untagged)-Human transmembrane and immunoglobulin domain containing 2 (TMIGD2), transcript variant 1 10 ug
$300.00

Plasmid Map for RC204938

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Cloning scheme for RC204938

Circular map for RC204938

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