LGALS3BP (NM_005567) Human Tagged ORF Clone
- TrueORF®
SKU
RC204918
LGALS3BP (Myc-DDK-tagged)-Human lectin, galactoside-binding, soluble, 3 binding protein (LGALS3BP)
$817.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | LGALS3BP |
| Synonyms | 90K; BTBD17B; CyCAP; gp90; M2BP; MAC-2-BP; TANGO10B |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_005567 |
| ORF Size | 1755 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_005567.2, NP_005558.1 |
| RefSeq Size | 2277 bp |
| RefSeq ORF | 1758 bp |
| Locus ID | 3959 |
| UniProt ID | Q08380, A0A0S2Z3Y1 |
| Cytogenetics | 17q25.3 |
| Domains | SR |
| Protein Families | Druggable Genome, Secreted Protein |
| MW | 65.3 kDa |
| Summary | The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. LGALS3BP has been found elevated in the serum of patients with cancer and in those infected by the human immunodeficiency virus (HIV). It appears to be implicated in immune response associated with natural killer (NK) and lymphokine-activated killer (LAK) cell cytotoxicity. Using fluorescence in situ hybridization the full length 90K cDNA has been localized to chromosome 17q25. The native protein binds specifically to a human macrophage-associated lectin known as Mac-2 and also binds galectin 1. provided by RefSeq, Jul 2008 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_005567" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC204918L1 | Lenti-ORF clone of LGALS3BP (Myc-DDK-tagged)-Human lectin, galactoside-binding, soluble, 3 binding protein (LGALS3BP) | 10 ug |
$1,117.00
|
|
| RC204918L2 | Lenti-ORF clone of LGALS3BP (mGFP-tagged)-Human lectin, galactoside-binding, soluble, 3 binding protein (LGALS3BP) | 10 ug |
$1,117.00
|
|
| RC204918L3 | Lenti-ORF clone of LGALS3BP (Myc-DDK-tagged)-Human lectin, galactoside-binding, soluble, 3 binding protein (LGALS3BP) | 10 ug |
$1,117.00
|
|
| RC204918L4 | Lenti-ORF clone of LGALS3BP (mGFP-tagged)-Human lectin, galactoside-binding, soluble, 3 binding protein (LGALS3BP) | 10 ug |
$1,117.00
|
|
| RG204918 | LGALS3BP (tGFP-tagged) - Human lectin, galactoside-binding, soluble, 3 binding protein (LGALS3BP) | 10 ug |
$1,017.00
|
|
| SC128088 | LGALS3BP (untagged)-Human lectin, galactoside-binding, soluble, 3 binding protein (LGALS3BP) | 10 ug |
$818.00
|
|
Citations
Galectin-3-binding protein inhibits extracellular heparan 6-O-endosulfatase Sulf-2
Panigrahi, Benicky, Aljuhani et al
Mol Cell Proteomics (2024)
Panigrahi, Benicky, Aljuhani et al
Mol Cell Proteomics (2024)
Cited by 3
AI Summary: OriGene’s Galectin-3-binding protein ORF clone (OriGene # RC204918) was used to generate recombinant LG3BP for validating its interaction with Sulf-2 and studying its inhibitory effect on Sulf-2 activity in this research.
Snippet: SULF2 ORF including C-terminal Myc-His tag was subcloned from its source vector (Addgene # 13003) to lentiviral transfer vector pHR-CMV-TetO2_3C-Twin-Strep (Addgene # 113883) (28). Galectin-3-binding protein ORF was subcloned from its source vector (Origene # RC204918) into bicistronic lentiviral transfer vector pHR-CMV-TetO2 3C-TwinStrep-IRES-EmGFP vector (Addgene, # 113884) modified to introduce 6xHis tag downstream of a cleavable C-terminal TwinStrep tag
Galectin-3-binding protein inhibits extracellular heparan 6-O-endosulfatse Sulf-2
Panigrahi, Benicky, Aljuhani et al
bioRxiv (2023)
Panigrahi, Benicky, Aljuhani et al
bioRxiv (2023)
Snippet: Galectin-3-binding protein ORF was subcloned from its source vector (Origene # RC204918) into bicistronic lentiviral transfer vector pHR-CMV-TetO2 3C-TwinStrepIRES-
SARS-CoV-2 RNAemia and proteomic trajectories inform prognostication in COVID-19 patients admitted to intensive care
Gutmann, Takov, Burnap et al
Nat Commun (2021) 12 (1), 3406 DOI: 10.1038/s41467-021-23494-1
Gutmann, Takov, Burnap et al
Nat Commun (2021) 12 (1), 3406 DOI: 10.1038/s41467-021-23494-1
Cited by 175
AI Summary: In this study, the OriGene product pCMV6-LGALS3BP (RC204918) was used to overexpress galectin-3-binding protein (LGALS3BP) in vitro, enabling the investigation of its role in inhibiting SARS-CoV-2 spike-pseudoparticle uptake and spike-induced cell-cell fusion.
Snippet: pMD2.G (Addgene, 12259) were obtained from Addgene and pCMV6-LGALS3BP (OriGene, RC204918) from ORIGENE
Lectin, galactoside-binding soluble 3 binding protein promotes 17-N-Allylamino-17-demethoxygeldanamycin resistance through PI3K/Akt pathway in lung cancer cell line
Woo, Jang, Kang et al
Mol. Cancer Ther. (2017)
Woo, Jang, Kang et al
Mol. Cancer Ther. (2017)
AI Summary: OriGene's human cDNA was used as the template for amplifying the LGALS3BP gene, enabling the researchers to construct plasmids for studying the role of LGALS3BP in 17-AAG resistance in lung cancer cells.
Snippet: 33). Plasmid construction and recombinant protein expression The gene encoding LGALS3BP was amplified from human cDNA (Origene, Rockville, MD, USA) construction and subcloned into either the pEGFP-N3 (Clontech, ... All 2 versions Cite SaveSaving
Quantitative proteomics reveals the roles of peroxisome-associated proteins in anti-viral innate immune responses
Zhou, Qin, Li et al
Mol. Cell Proteomics (2015)
Zhou, Qin, Li et al
Mol. Cell Proteomics (2015)
AI Summary: OriGene’s human cDNA expression plasmids from their cDNA library were used to functionally analyze candidate peroxisome-associated proteins, enabling the identification of LGALS3BP and CALU as potential negative regulators of virus-induced type I interferon activation.
Snippet: 2) The candidate protein function was unknown or its previous reported function was unrelated to host immune responses. 3) The human cDNA expression plasmid is readily available in an Origene cDNA library. By following ... Cite SaveSaving
Showing 1-5 of 5 papers.
Powered by BizGenius
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
Related Products
- Price:
- Actual Price:
Welcome to OriGene AI!
I'm your AI-powered guide to everything OriGene.
Ask me anything:
- Product specs
- Ordering help
- Technical questions
- How to find exactly what your experiment needs
Prefer a live agent? Connect with us by clicking the green chat icon, as seen in the image below or email our team at custsupport@origene.com.
