OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

Rsafd1 (TYW1) (NM_018264) Human Tagged ORF Clone

  • TrueORF®
Be the first to review this product
SKU
RC204851
TYW1 (Myc-DDK-tagged)-Human tRNA-yW synthesizing protein 1 homolog (S. cerevisiae) (TYW1)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $474.00
4 Weeks*
Bulk Requests & Clone Modifications

Frequently Bought Together (6)

TA319957 Rabbit Polyclonal TYW1 Antibody
100 ug
$640.00
TA592569S DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
30 ul
$200.00
CC100003 DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
5 x 200 ul
$170.00
PRIMER Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
100 pmol
$59.00
PS100001 pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
10 ug
$670.00
AR100028M DDKTrap Anti-DDK 8G6 affinity resin used for the purification of DDK, DYKDDDDK, or FLAG® tagged proteins , 5 mL
5 ml
$750.00

Other products for "Rsafd1 or NM_018264"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol Rsafd1
Synonyms RSAFD1; TYW1A; YPL207W
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC204851 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGATCCTTCTGCGGATACATGGGACCTCTTCTCACCTTTAATATCATTATGGATAAACAGGTTTTACA
TTTATTTGGGCTTTGCTGTTAGCATTAGCCTTTGGATTTGTGTCCAGATTGTCATCAAGACGCAGGGCAA
GAACTTACAGGAAAAATCTGTTCCAAAAGCAGCTCAGGATTTGATGACAAATGGTTATGTCTCCCTTCAA
GAGAAAGACATCTTTGTGTCTGGAGTGAAGATTTTTTATGGTTCTCAGACTGGAACAGCGAAGGGATTCG
CAACAGTTCTTGCTGAAGCAGTTACATCCCTGGATCTGCCTGTGGCCATTATTAATCTAAAAGAATATGA
TCCAGATGATCATCTGATAGAAGAGGTGACTAGTAAAAATGTCTGTGTCTTCCTGGTTGCGACATACACT
GACGGCCTACCAACTGAAAGTGCAGAGTGGTTCTGCAAATGGTTAGAGGAAGCATCCATTGATTTTCGAT
TTGGCAAAACTTACCTGAAGGGTATGAGATATGCGGTATTTGGCCTGGGAAATTCTGCCTATGCTAGCCA
CTTCAACAAGGTTGGCAAAAATGTTGACAAGTGGCTCTGGATGCTTGGCGCGCATCGTGTGATGAGTCGA
GGGGAGGGCGACTGCGACGTGGTTAAAAGCAAGCACGGCAGCATTGAGGCCGACTTCAGAGCATGGAAGA
CCAAGTTCATCTCCCAGCTGCAGGCACTTCAGAAAGGGGAGAGAAAGAAGTCCTGTGGCGGCCACTGCAA
GAAAGGCAAATGTGAATCTCACCAACATGGCTCAGAGGAGAGGGAGGAAGGATCTCATGAGCAGGATGAA
TTGCATCATAGAGACACCGAGGAGGAAGAACCCTTTGAGAGCTCCAGTGAAGAAGAGTTTGGTGGTGAGG
ACCATCAGAGCCTAAATTCCATTGTTGATGTTGAAGATTTGGGCAAAATTATGGATCATGTGAAGAAAGA
AAAGAGAGAAAAGGAACAGCAGGAAGAGAAGTCTGGTTTGTTCAGGAACATGGGGAGGAATGAAGATGGT
GAAAGAAGAGCTATGATAACTCCTGCTCTCCGAGAAGCCCTTACTAAACAAGGTTATCAGTTGATTGGGA
GCCACTCGGGGGTGAAGCTTTGCAGGTGGACAAAGTCCATGCTCCGAGGGAGAGGAGGTTGTTACAAACA
CACATTCTATGGAATTGAGAGCCATCGCTGCATGGAAACCACCCCGAGCTTGGCGTGTGCTAATAAATGT
GTCTTCTGTTGGCGGCACCACACCAACCCCGTGGGCACTGAGTGGCGGTGGAAGATGGACCAGCCTGAAA
TGATCTTGAAGGAAGCCATTGAAAACCATCAGAACATGATTAAGCAGTTTAAAGGAGTACCGGGCGTCAA
AGCAGAACGCTTTGAAGAAGGAATGACGGTAAAGCACTGTGCATTGTCCCTCGTGGGAGAACCAATAATG
TACCCAGAGATCAACAGGTTTTTGAAGCTACTCCACCAGTGTAAAATTTCCAGCTTCCTGGTCACAAACG
CACAATTTCCTGCGGAAATCAGGAACCTCGAGCCGGTTACTCAGCTGTATGTCAGTGTGGATGCCAGTAC
CAAAGACAGCCTGAAGAAAATCGACCGCCCACTCTTCAAGGATTTCTGGCAGAGATTCCTTGACAGTTTA
AAAGCCTTGGCAGTCAAGCAACAACGAACTGTCTACAGACTGACGCTCGTGAAAGCATGGAACGTGGACG
AGCTCCAGGCCTACGCGCAGCTCGTGTCCCTGGGGAATCCTGACTTCATCGAAGTGAAGGGCGTTACCTA
CTGCGGAGAAAGTTCAGCAAGCAGTCTTACCATGGCCCACGTGCCCTGGCATGAGGAAGTGGTACAGTTT
GTCCACGAGTTGGTGGATCTGATCCCCGAATATGAAATTGCATGTGAACACGAACACTCTAATTGCCTCC
TGATAGCACACAGAAAGTTTAAAATTGTGGTGAATGGTGGACATGGATCGATTATAACCGCTTCCAGGAG
CTCATCCAGGAATATGAAGATAGTGGTGGATCAAAAACGTTCAGCGCAAAGGATTATATGGCCAGAACTC
CTCACTGGGCATTATTTGGTGCCAGTGAAAGAGGCTTTGATCCCAAGGACACAAGACATCAGAGAAAGAA
CAAATCAAAGGCTATTTCTGGATGTTGAGATTATC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC204851 protein sequence
Red=Cloning site Green=Tags(s)

MDPSADTWDLFSPLISLWINRFYIYLGFAVSISLWICVQIVIKTQGKNLQEKSVPKAAQDLMTNGYVSLQ
EKDIFVSGVKIFYGSQTGTAKGFATVLAEAVTSLDLPVAIINLKEYDPDDHLIEEVTSKNVCVFLVATYT
DGLPTESAEWFCKWLEEASIDFRFGKTYLKGMRYAVFGLGNSAYASHFNKVGKNVDKWLWMLGAHRVMSR
GEGDCDVVKSKHGSIEADFRAWKTKFISQLQALQKGERKKSCGGHCKKGKCESHQHGSEEREEGSHEQDE
LHHRDTEEEEPFESSSEEEFGGEDHQSLNSIVDVEDLGKIMDHVKKEKREKEQQEEKSGLFRNMGRNEDG
ERRAMITPALREALTKQGYQLIGSHSGVKLCRWTKSMLRGRGGCYKHTFYGIESHRCMETTPSLACANKC
VFCWRHHTNPVGTEWRWKMDQPEMILKEAIENHQNMIKQFKGVPGVKAERFEEGMTVKHCALSLVGEPIM
YPEINRFLKLLHQCKISSFLVTNAQFPAEIRNLEPVTQLYVSVDASTKDSLKKIDRPLFKDFWQRFLDSL
KALAVKQQRTVYRLTLVKAWNVDELQAYAQLVSLGNPDFIEVKGVTYCGESSASSLTMAHVPWHEEVVQF
VHELVDLIPEYEIACEHEHSNCLLIAHRKFKIVVNGGHGSIITASRSSSRNMKIVVDQKRSAQRIIWPEL
LTGHYLVPVKEALIPRTQDIRERTNQRLFLDVEII

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_018264
ORF Size 2205 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_018264.4
RefSeq Size 3364 bp
RefSeq ORF 2199 bp
Locus ID 55253
UniProt ID Q9NV66
Cytogenetics 7q11.21
Domains flavodoxin, Radical_SAM
Protein Families Transmembrane
MW 83.8 kDa
Summary Wybutosine (yW) is a hypermodified guanosine found in phenylalanine tRNA adjacent to the anticodon that stabilizes codon-anticodon interactions in the ribosome. In yeast, the homolog of this gene is essential for the synthesis of wybutosine. Alternative splicing results in multiple transcript variants. provided by RefSeq, Dec 2015
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
"NM_018264" in other vectors (4)
SKU Description Size Price
RC204851L3 Lenti-ORF clone of TYW1 (Myc-DDK-tagged)-Human tRNA-yW synthesizing protein 1 homolog (S. cerevisiae) (TYW1) 10 ug
$774.00
RC204851L4 Lenti-ORF clone of TYW1 (mGFP-tagged)-Human tRNA-yW synthesizing protein 1 homolog (S. cerevisiae) (TYW1) 10 ug
$774.00
RG204851 TYW1 (tGFP-tagged) - Human tRNA-yW synthesizing protein 1 homolog (S. cerevisiae) (TYW1) 10 ug
$674.00
SC113583 TYW1 (untagged)-Human tRNA-yW synthesizing protein 1 homolog (S. cerevisiae) (TYW1) 10 ug
$671.00

Plasmid Map for RC204851

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC204851
Circular map for RC204851

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products