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HSD17B6 (NM_003725) Human Tagged ORF Clone

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SKU
RC204701
HSD17B6 (Myc-DDK-tagged)-Human hydroxysteroid (17-beta) dehydrogenase 6 homolog (mouse) (HSD17B6)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol HSD17B6
Synonyms HSE; RODH; SDR9C6
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC204701 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTGGCTCTACCTGGCGGCCTTCGTGGGCCTGTACTACCTTCTGCACTGGTACCGGGAGAGGCAGGTGG
TGAGCCACCTCCAAGACAAGTATGTCTTTATCACGGGCTGTGACTCGGGCTTTGGGAACCTGCTGGCCAG
ACAGCTGGATGCACGAGGCTTGAGAGTGCTGGCTGCGTGTCTGACGGAGAAGGGGGCCGAGCAGCTGAGG
GGCCAGACGTCTGACAGGCTGGAGACGGTGACCCTGGATGTTACCAAGATGGAGAGCATCGCTGCAGCTA
CTCAGTGGGTGAAGGAGCATGTGGGGGACAGAGGACTCTGGGGACTGGTGAACAATGCAGGCATTCTTAC
ACCAATTACCTTATGTGAGTGGCTGAACACTGAGGACTCTATGAATATGCTCAAAGTGAACCTCATTGGT
GTGATCCAGGTGACCTTGAGCATGCTTCCTTTGGTGAGGAGAGCACGGGGAAGAATTGTCAATGTCTCCA
GCATTCTGGGAAGAGTTGCTTTCTTTGTAGGAGGCTACTGTGTCTCCAAGTATGGAGTGGAAGCCTTTTC
AGATATTCTGAGGCGTGAGATTCAACATTTTGGGGTGAAAATCAGCATAGTTGAACCTGGCTACTTCAGA
ACGGGAATGACAAACATGACACAGTCCTTAGAGCGAATGAAGCAAAGTTGGAAAGAAGCCCCCAAGCATA
TTAAGGAGACCTATGGACAGCAGTATTTTGATGCCCTTTACAATATCATGAAGGAAGGGCTGTTGAATTG
TAGCACAAACCTGAACCTGGTCACTGACTGCATGGAACATGCTCTGACATCGGTGCATCCGCGAACTCGA
TATTCAGCTGGCTGGGATGCTAAATTTTTCTTCATCCCTCTATCTTATTTACCTACATCACTGGCAGACT
ACATTTTGACTAGATCTTGGCCCAAACCAGCCCAGGCAGTC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC204701 protein sequence
Red=Cloning site Green=Tags(s)

MWLYLAAFVGLYYLLHWYRERQVVSHLQDKYVFITGCDSGFGNLLARQLDARGLRVLAACLTEKGAEQLR
GQTSDRLETVTLDVTKMESIAAATQWVKEHVGDRGLWGLVNNAGILTPITLCEWLNTEDSMNMLKVNLIG
VIQVTLSMLPLVRRARGRIVNVSSILGRVAFFVGGYCVSKYGVEAFSDILRREIQHFGVKISIVEPGYFR
TGMTNMTQSLERMKQSWKEAPKHIKETYGQQYFDALYNIMKEGLLNCSTNLNLVTDCMEHALTSVHPRTR
YSAGWDAKFFFIPLSYLPTSLADYILTRSWPKPAQAV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_003725
ORF Size 951 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_003725.4
RefSeq Size 1629 bp
RefSeq ORF 954 bp
Locus ID 8630
UniProt ID O14756
Cytogenetics 12q13.3
Domains adh_short
Protein Families Druggable Genome
MW 36 kDa
Summary The protein encoded by this gene has both oxidoreductase and epimerase activities and is involved in androgen catabolism. The oxidoreductase activity can convert 3 alpha-adiol to dihydrotestosterone, while the epimerase activity can convert androsterone to epi-androsterone. Both reactions use NAD+ as the preferred cofactor. This gene is a member of the retinol dehydrogenase family. provided by RefSeq, Aug 2013
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Documents
Resources
"NM_003725" in other vectors (7)
SKU Description Size Price
RC204701L1 Lenti ORF clone of Human hydroxysteroid (17-beta) dehydrogenase 6 homolog (mouse) (HSD17B6), Myc-DDK-tagged 10 ug
$600.00
RC204701L2 Lenti ORF clone of Human hydroxysteroid (17-beta) dehydrogenase 6 homolog (mouse) (HSD17B6), mGFP tagged 10 ug
$600.00
RC204701L3 Lenti ORF clone of Human hydroxysteroid (17-beta) dehydrogenase 6 homolog (mouse) (HSD17B6), Myc-DDK-tagged 10 ug
$600.00
RC204701L4 Lenti ORF clone of Human hydroxysteroid (17-beta) dehydrogenase 6 homolog (mouse) (HSD17B6), mGFP tagged 10 ug
$600.00
RG204701 HSD17B6 (tGFP-tagged) - Human hydroxysteroid (17-beta) dehydrogenase 6 homolog (mouse) (HSD17B6) 10 ug
$489.00 $500.00
SC117792 HSD17B6 (untagged)-Human hydroxysteroid (17-beta) dehydrogenase 6 homolog (mouse) (HSD17B6) 10 ug
$300.00
SC324525 HSD17B6 (untagged)-Human hydroxysteroid (17-beta) dehydrogenase 6 homolog (mouse) (HSD17B6) 10 ug
$300.00

Plasmid Map for RC204701

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC204701
Circular map for RC204701

file-alt Citations

Inhibition of dihydrotestosterone synthesis in prostate cancer by combined frontdoor and backdoor pathway blockade
Fiandalo, Stocking, Pop et al
Oncotarget (2018) 9 (13), 11227-11242 DOI: 10.18632/oncotarget.24107
Snippet: and Strategene's protocol. pCMV6-entry expression plasmids with C-terminal MYC-DDK tag encoded with HSD17B6, RDH16, DHRS9 or RDH5 were purchased from Origene (Origene, Rockville, MD). 3α-oxidoreductase primers
FigureFigureFigure
Showing 1-1 of 1 papers.
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