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CASQ2 (NM_001232) Human Tagged ORF Clone

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SKU
RC204695
CASQ2 (Myc-DDK-tagged)-Human calsequestrin 2 (cardiac muscle) (CASQ2), nuclear gene encoding mitochondrial protein
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $457.00
In Stock*
Bulk Requests & Clone Modifications

Frequently Bought Together (6)

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Other products for "CASQ2 or NM_001232"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol CASQ2
Synonyms PDIB2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC204695 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGAGAACTCACTTGTTTATTGTGGGGATTTATTTTCTGTCCTCTTGCAGGGCAGAAGAGGGGCTTA
ATTTCCCCACATATGATGGGAAGGACCGAGTGGTAAGTCTTTCCGAGAAGAACTTCAAGCAGGTTTTAAA
GAAATATGACTTGCTTTGCCTCTACTACCATGAGCCGGTGTCTTCAGATAAGGTCACGCAAAAACAGTTC
CAACTGAAAGAAATCGTGCTTGAGCTTGTGGCCCAGGTCCTTGAACATAAAGCTATAGGCTTTGTGATGG
TGGATGCCAAGAAAGAAGCCAAGCTTGCCAAGAAACTGGGTTTTGATGAAGAAGGAAGCCTGTATATTCT
TAAGGGTGATCGCACAATAGAGTTTGATGGCGAGTTTGCAGCTGATGTCTTGGTGGAGTTCCTCTTGGAT
CTAATTGAAGACCCAGTGGAGATCATCAGCAGCAAACTGGAAGTCCAAGCCTTCGAACGCATTGAAGACT
ACATCAAACTCATTGGCTTTTTCAAGAGTGAGGACTCAGAATACTACAAGGCTTTTGAAGAAGCAGCTGA
ACACTTCCAGCCTTACATCAAATTCTTTGCCACCTTTGACAAAGGGGTTGCAAAGAAATTATCTTTGAAG
ATGAATGAGGTTGACTTCTATGAGCCATTTATGGATGAGCCCATTGCCATCCCCAACAAACCTTACACAG
AAGAGGAGCTGGTGGAGTTTGTGAAGGAACACCAAAGACCCACTCTACGTCGCCTGCGCCCAGAAGAAAT
GTTTGAAACATGGGAAGATGATTTGAATGGGATCCACATTGTGGCCTTTGCAGAGAAGAGTGATCCAGAT
GGCTACGAATTCCTGGAGATCCTGAAACAGGTTGCCCGGGACAATACTGACAACCCCGATCTGAGCATCC
TGTGGATCGACCCGGACGACTTTCCTCTGCTCGTTGCCTACTGGGAGAAGACTTTCAAGATTGACCTATT
CAGGCCACAGATTGGGGTGGTGAATGTCACAGATGCTGACAGTGTCTGGATGGAGATTCCAGATGATGAC
GATCTTCCAACTGCTGAGGAGCTGGAGGACTGGATTGAGGATGTGCTTTCTGGAAAGATAAACACTGAAG
ATGATGATGAAGATGATGATGATGATGATAATTCTGATGAAGAGGATAATGATGACAGTGATGATGATGA
TGATGAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC204695 protein sequence
Red=Cloning site Green=Tags(s)

MKRTHLFIVGIYFLSSCRAEEGLNFPTYDGKDRVVSLSEKNFKQVLKKYDLLCLYYHEPVSSDKVTQKQF
QLKEIVLELVAQVLEHKAIGFVMVDAKKEAKLAKKLGFDEEGSLYILKGDRTIEFDGEFAADVLVEFLLD
LIEDPVEIISSKLEVQAFERIEDYIKLIGFFKSEDSEYYKAFEEAAEHFQPYIKFFATFDKGVAKKLSLK
MNEVDFYEPFMDEPIAIPNKPYTEEELVEFVKEHQRPTLRRLRPEEMFETWEDDLNGIHIVAFAEKSDPD
GYEFLEILKQVARDNTDNPDLSILWIDPDDFPLLVAYWEKTFKIDLFRPQIGVVNVTDADSVWMEIPDDD
DLPTAEELEDWIEDVLSGKINTEDDDEDDDDDDNSDEEDNDDSDDDDDE

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001232
ORF Size 1197 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001232.4
RefSeq Size 2716 bp
RefSeq ORF 1200 bp
Locus ID 845
UniProt ID O14958
Cytogenetics 1p13.1
Domains Calsequestrin
Protein Families Druggable Genome
MW 46.4 kDa
Summary The protein encoded by this gene specifies the cardiac muscle family member of the calsequestrin family. Calsequestrin is localized to the sarcoplasmic reticulum in cardiac and slow skeletal muscle cells. The protein is a calcium binding protein that stores calcium for muscle function. Mutations in this gene cause stress-induced polymorphic ventricular tachycardia, also referred to as catecholaminergic polymorphic ventricular tachycardia 2 (CPVT2), a disease characterized by bidirectional ventricular tachycardia that may lead to cardiac arrest. provided by RefSeq, Jul 2008
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"NM_001232" in other vectors (6)
SKU Description Size Price
RC204695L1 Lenti ORF clone of Human calsequestrin 2 (cardiac muscle) (CASQ2), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged 10 ug
$757.00
RC204695L2 Lenti ORF clone of Human calsequestrin 2 (cardiac muscle) (CASQ2), nuclear gene encoding mitochondrial protein, mGFP tagged 10 ug
$757.00
RC204695L3 Lenti ORF clone of Human calsequestrin 2 (cardiac muscle) (CASQ2), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged 10 ug
$757.00
RC204695L4 Lenti ORF clone of Human calsequestrin 2 (cardiac muscle) (CASQ2), nuclear gene encoding mitochondrial protein, mGFP tagged 10 ug
$757.00
RG204695 CASQ2 (tGFP-tagged) - Human calsequestrin 2 (cardiac muscle) (CASQ2), nuclear gene encoding mitochondrial protein 10 ug
$489.00 $657.00
SC119365 CASQ2 (untagged)-Human calsequestrin 2 (cardiac muscle) (CASQ2), nuclear gene encoding mitochondrial protein 10 ug
$457.00

Plasmid Map for RC204695

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC204695
Circular map for RC204695

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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