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SLC25A32 (NM_030780) Human Tagged ORF Clone

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SKU
RC204580
SLC25A32 (Myc-DDK-tagged)-Human solute carrier family 25, member 32 (SLC25A32), nuclear gene encoding mitochondrial protein
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol SLC25A32
Synonyms GLYB; MFT; MFTC; RREI
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC204580 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACGGGCCAGGGCCAGTCGGCGTCCGGGTCGTCGGCGTGGAGCACGGTATTCCGCCACGTCCGGTATG
AGAACCTGATAGCGGGCGTGAGCGGCGGCGTCTTATCCAACCTTGCGCTGCATCCGCTCGACCTCGTGAA
GATCCGCTTCGCCGTGAGTGATGGATTGGAACTGAGACCGAAATATAATGGAATTTTACATTGCTTGACT
ACCATTTGGAAACTTGATGGACTACGGGGACTTTATCAAGGAGTAACCCCAAATATATGGGGTGCAGGTT
TATCCTGGGGACTCTACTTTTTCTTTTACAATGCCATCAAGTCATATAAAACAGAAGGAAGAGCTGAACG
TTTAGAGGCAACAGAATACCTTGTCTCAGCTGCTGAAGCTGGAGCCATGACCCTCTGCATTACAAACCCA
TTATGGGTAACAAAAACTCGCCTTATGTTACAGTATGATGCTGTTGTTAACTCCCCACACCGACAATATA
AAGGAATGTTTGATACACTTGTGAAAATATATAAGTATGAAGGTGTGCGTGGATTATATAAGGGATTTGT
TCCTGGGCTGTTTGGAACATCGCATGGTGCCCTTCAGTTTATGGCATATGAATTGCTGAAGTTGAAGTAC
AACCAGCATATCAATAGATTACCAGAAGCCCAGTTGAGCACAGTAGAATATATATCTGTTGCAGCACTAT
CCAAAATATTTGCTGTCGCAGCAACATACCCATATCAAGTCGTAAGAGCTCGTCTTCAGGATCAACACAT
GTTTTACAGTGGTGTAATAGATGTAATCACAAAGACATGGAGGAAAGAAGGCGTCGGTGGATTTTACAAG
GGAATTGCTCCTAATTTGATTAGAGTGACTCCAGCCTGCTGTATTACCTTTGTGGTATATGAAAACGTCT
CACATTTTTTACTTGACCTTAGAGAAAAGAGAAAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC204580 protein sequence
Red=Cloning site Green=Tags(s)

MTGQGQSASGSSAWSTVFRHVRYENLIAGVSGGVLSNLALHPLDLVKIRFAVSDGLELRPKYNGILHCLT
TIWKLDGLRGLYQGVTPNIWGAGLSWGLYFFFYNAIKSYKTEGRAERLEATEYLVSAAEAGAMTLCITNP
LWVTKTRLMLQYDAVVNSPHRQYKGMFDTLVKIYKYEGVRGLYKGFVPGLFGTSHGALQFMAYELLKLKY
NQHINRLPEAQLSTVEYISVAALSKIFAVAATYPYQVVRARLQDQHMFYSGVIDVITKTWRKEGVGGFYK
GIAPNLIRVTPACCITFVVYENVSHFLLDLREKRK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_030780
ORF Size 945 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_030780.5
RefSeq Size 3133 bp
RefSeq ORF 948 bp
Locus ID 81034
UniProt ID Q9H2D1
Cytogenetics 8q22.3
Domains mito_carr
Protein Families Druggable Genome
MW 35.4 kDa
Summary This gene encodes a member of the P(I/L)W subfamily of mitochondrial carrier family transport proteins. The encoded protein transports folate across the inner mitochondrial membrane. Alternatively spliced transcript variants have been described. provided by RefSeq, Mar 2013
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"NM_030780" in other vectors (7)
SKU Description Size Price
RC204580L1 Lenti ORF clone of Human solute carrier family 25, member 32 (SLC25A32), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged 10 ug
$600.00
RC204580L2 Lenti ORF clone of Human solute carrier family 25, member 32 (SLC25A32), nuclear gene encoding mitochondrial protein, mGFP tagged 10 ug
$600.00
RC204580L3 Lenti ORF clone of Human solute carrier family 25, member 32 (SLC25A32), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged 10 ug
$600.00
RC204580L4 Lenti ORF clone of Human solute carrier family 25, member 32 (SLC25A32), nuclear gene encoding mitochondrial protein, mGFP tagged 10 ug
$600.00
RG204580 SLC25A32 (tGFP-tagged) - Human solute carrier family 25, member 32 (SLC25A32), nuclear gene encoding mitochondrial protein 10 ug
$500.00
SC109458 SLC25A32 (untagged)-Human solute carrier family 25, member 32 (SLC25A32), nuclear gene encoding mitochondrial protein 10 ug
$300.00
SC322536 SLC25A32 (untagged)-Human solute carrier family 25, member 32 (SLC25A32), nuclear gene encoding mitochondrial protein 10 ug
$300.00

Plasmid Map for RC204580

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC204580
Circular map for RC204580

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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