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YIPF6 (NM_173834) Human Tagged ORF Clone

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SKU
RC204519
YIPF6 (Myc-DDK-tagged)-Human Yip1 domain family, member 6 (YIPF6), transcript variant A
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol YIPF6
Synonyms FinGER6
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC204519 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGAAGCGGAGGAGTCTCCAGGAGACCCGGGGACAGCATCGCCCAGGCCCCTGTTTGCAGGCCTTT
CAGATATATCCATCTCACAAGACATCCCCGTAGAAGGAGAAATCACCATTCCTATGAGATCTCGCATCCG
GGAGTTTGACAGCTCCACATTAAATGAATCTGTTCGCAATACCATCATGCGTGATCTAAAAGCTGTTGGG
AAAAAATTCATGCATGTTTTGTACCCAAGGAAAAGTAATACTCTTTTGAGAGATTGGGATTTGTGGGGCC
CTTTGATCCTTTGTGTGACACTCGCATTAATGCTGCAAAGAGACTCTGCAGATAGTGAAAAAGATGGAGG
GCCCCAATTTGCAGAGGTGTTTGTCATTGTCTGGTTTGGTGCAGTTACCATCACCCTCAACTCAAAACTT
CTTGGAGGGAACATATCTTTTTTTCAGAGCCTCTGTGTGCTGGGTTACTGTATACTTCCCTTGACAGTAG
CAATGCTGATTTGCCGGCTGGTACTTTTGGCTGATCCAGGACCTGTAAACTTCATGGTTCGGCTTTTTGT
GGTGATTGTGATGTTTGCCTGGTCTATAGTTGCCTCCACAGCTCTCCTTGCTGATAGCCAGCCTCCAAAC
CGCAGAGCCCTAGCTGTTTATCCTGTTTTCCTGTTTTACTTTGTCATCAGTTGGATGATTCTCACCTTTA
CTCCTCAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC204519 protein sequence
Red=Cloning site Green=Tags(s)

MAEAEESPGDPGTASPRPLFAGLSDISISQDIPVEGEITIPMRSRIREFDSSTLNESVRNTIMRDLKAVG
KKFMHVLYPRKSNTLLRDWDLWGPLILCVTLALMLQRDSADSEKDGGPQFAEVFVIVWFGAVTITLNSKL
LGGNISFFQSLCVLGYCILPLTVAMLICRLVLLADPGPVNFMVRLFVVIVMFAWSIVASTALLADSQPPN
RRALAVYPVFLFYFVISWMILTFTPQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_173834
ORF Size 708 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_173834.2, NP_776195.1
RefSeq Size 6282 bp
RefSeq ORF 711 bp
Locus ID 286451
UniProt ID Q96EC8
Cytogenetics Xq12-q13.1
Protein Families Transmembrane
MW 26.2 kDa
Summary May be required for stable YIPF1 and YIPF2 protein expression.UniProtKB/Swiss-Prot Function
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Documents
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"NM_173834" in other vectors (5)
SKU Description Size Price
RC204519L3 Lenti ORF clone of Human Yip1 domain family, member 6 (YIPF6), transcript variant A, Myc-DDK-tagged 10 ug
$600.00
RC204519L4 Lenti ORF clone of Human Yip1 domain family, member 6 (YIPF6), transcript variant A, mGFP tagged 10 ug
$600.00
RG204519 YIPF6 (tGFP-tagged) - Human Yip1 domain family, member 6 (YIPF6), transcript variant A 10 ug
$489.00 $500.00
SC106944 YIPF6 (untagged)-Human Yip1 domain family, member 6 (YIPF6), transcript variant A 10 ug
$300.00
SC323925 YIPF6 (untagged)-Human Yip1 domain family, member 6 (YIPF6), transcript variant A 10 ug
$300.00

Plasmid Map for RC204519

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC204519
Circular map for RC204519

file-alt Citations

Yip1 domain family, member 6 (Yipf6) mutation induces spontaneous intestinal inflammation in mice
Brandl, Tomisato, Li et al
PNAS (2012) 109 (31), 12650-5 DOI: 10.1073/pnas.1210366109
Cited by 40
AI Summary: OriGene’s Yipf6-GFP plasmid (MG202877) was used in this study to investigate the cellular localization and function of Yipf6, helping to elucidate its role in intestinal homeostasis and inflammation.
Snippet: cells. However, homeostatic mechanisms normally restore the status quo ante, so that dysfunction is short-lived. These ... ATTGGTCCCTGTCCAGCCAAAAG. Yipf6-GFP plasmid was purchased from Origene (MG202877). Kdel
Showing 1-1 of 1 papers.
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