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beta 2 Adrenergic Receptor (ADRB2) (NM_000024) Human Tagged ORF Clone

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SKU
RC204499
ADRB2 (Myc-DDK-tagged)-Human adrenergic, beta-2-, receptor, surface (ADRB2)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
  $686.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol beta 2 Adrenergic Receptor
Synonyms ADRB2R; ADRBR; B2AR; BAR; BETA2AR
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC204499 representing NM_000024
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGCAACCCGGGAACGGCAGCGCCTTCTTGCTGGCACCCAATGGAAGCCATGCGCCGGACCACGACG
TCACGCAGCAAAGGGACGAGGTGTGGGTGGTGGGCATGGGCATCGTCATGTCTCTCATCGTCCTGGCCAT
CGTGTTTGGCAATGTGCTGGTCATCACAGCCATTGCCAAGTTCGAGCGTCTGCAGACGGTCACCAACTAC
TTCATCACTTCACTGGCCTGTGCTGATCTGGTCATGGGCCTAGCAGTGGTGCCCTTTGGGGCCGCCCATA
TTCTTATGAAAATGTGGACTTTTGGCAACTTCTGGTGCGAGTTTTGGACTTCCATTGATGTGCTGTGCGT
CACGGCCAGCATTGAGACCCTGTGCGTGATCGCAGTGGATCGCTACTTTGCCATTACTTCACCTTTCAAG
TACCAGAGCCTGCTGACCAAGAATAAGGCCCGGGTGATCATTCTGATGGTGTGGATTGTGTCAGGCCTTA
CCTCCTTCTTGCCCATTCAGATGCACTGGTACAGGGCCACCCACCAGGAAGCCATCAACTGCTATGCCAA
TGAGACCTGCTGTGACTTCTTCACGAACCAAGCCTATGCCATTGCCTCTTCCATCGTGTCCTTCTACGTT
CCCCTGGTGATCATGGTCTTCGTCTACTCCAGGGTCTTTCAGGAGGCCAAAAGGCAGCTCCAGAAGATTG
ACAAATCTGAGGGCCGCTTCCATGTCCAGAACCTTAGCCAGGTGGAGCAGGATGGGCGGACGGGGCATGG
ACTCCGCAGATCTTCCAAGTTCTGCTTGAAGGAGCACAAAGCCCTCAAGACGTTAGGCATCATCATGGGC
ACTTTCACCCTCTGCTGGCTGCCCTTCTTCATCGTTAACATTGTGCATGTGATCCAGGATAACCTCATCC
GTAAGGAAGTTTACATCCTCCTAAATTGGATAGGCTATGTCAATTCTGGTTTCAATCCCCTTATCTACTG
CCGGAGCCCAGATTTCAGGATTGCCTTCCAGGAGCTTCTGTGCCTGCGCAGGTCTTCTTTGAAGGCCTAT
GGCAATGGCTACTCCAGCAACGGCAACACAGGGGAGCAGAGTGGATATCACGTGGAACAGGAGAAAGAAA
ATAAACTGCTGTGTGAAGACCTCCCAGGCACGGAAGACTTTGTGGGCCATCAAGGTACTGTGCCTAGCGA
TAACATTGATTCACAAGGGAGGAATTGTAGTACAAATGACTCACTGCTA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC204499 protein sequence
Red=Cloning site Green=Tags(s)

MGQPGNGSAFLLAPNGSHAPDHDVTQQRDEVWVVGMGIVMSLIVLAIVFGNVLVITAIAKFERLQTVTNY
FITSLACADLVMGLAVVPFGAAHILMKMWTFGNFWCEFWTSIDVLCVTASIETLCVIAVDRYFAITSPFK
YQSLLTKNKARVIILMVWIVSGLTSFLPIQMHWYRATHQEAINCYANETCCDFFTNQAYAIASSIVSFYV
PLVIMVFVYSRVFQEAKRQLQKIDKSEGRFHVQNLSQVEQDGRTGHGLRRSSKFCLKEHKALKTLGIIMG
TFTLCWLPFFIVNIVHVIQDNLIRKEVYILLNWIGYVNSGFNPLIYCRSPDFRIAFQELLCLRRSSLKAY
GNGYSSNGNTGEQSGYHVEQEKENKLLCEDLPGTEDFVGHQGTVPSDNIDSPGRNCSTNDSLL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene |
ACCN NM_000024
ORF Size 1239 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_000024.6
RefSeq Size 2015 bp
RefSeq ORF 1242 bp
Locus ID 154
UniProt ID P07550
Cytogenetics 5q32
Domains 7tm_1
Protein Families Druggable Genome, GPCR, Transmembrane
Protein Pathways Calcium signaling pathway, Endocytosis, Neuroactive ligand-receptor interaction
MW 46.4 kDa
Summary This gene encodes beta-2-adrenergic receptor which is a member of the G protein-coupled receptor superfamily. This receptor is directly associated with one of its ultimate effectors, the class C L-type calcium channel Ca(V)1.2. This receptor-channel complex also contains a G protein, an adenylyl cyclase, cAMP-dependent kinase, and the counterbalancing phosphatase, PP2A. The assembly of the signaling complex provides a mechanism that ensures specific and rapid signaling by this G protein-coupled receptor. This receptor is also a transcription regulator of the alpha-synuclein gene, and together, both genes are believed to be associated with risk of Parkinson's Disease. This gene is intronless. Different polymorphic forms, point mutations, and/or downregulation of this gene are associated with nocturnal asthma, obesity, type 2 diabetes and cardiovascular disease. provided by RefSeq, Oct 2019
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Documents
Resources
"NM_000024" in other vectors (6)
SKU Description Size Price
RC204499L1 Lenti ORF clone of Human adrenergic, beta-2-, receptor, surface (ADRB2), Myc-DDK-tagged 10 ug
$986.00
RC204499L2 Lenti ORF clone of Human adrenergic, beta-2-, receptor, surface (ADRB2), mGFP tagged 10 ug
$986.00
RC204499L3 Lenti ORF clone of Human adrenergic, beta-2-, receptor, surface (ADRB2), Myc-DDK-tagged 10 ug
$986.00
RC204499L4 Lenti ORF clone of Human adrenergic, beta-2-, receptor, surface (ADRB2), mGFP tagged 10 ug
$986.00
RG204499 ADRB2 (tGFP-tagged) - Human adrenergic, beta-2-, receptor, surface (ADRB2) 10 ug
$886.00
SC116997 ADRB2 (untagged)-Human adrenergic, beta-2-, receptor, surface (ADRB2) 10 ug
$686.00

file-alt Citations

PKA phosphorylation of SUR2B subunit underscores vascular KATP channel activation by beta-adrenergic receptors
Shi, Wu, Cui et al
Am J Physiol Regul Integr Comp Physiol (2007) 293 (3), R1205-14 DOI: 10.1152/ajpregu.00337.2007
Cited by 80
AI Summary: OriGene products contributed to this study by providing the Kir6.1 (Cat# SC116827) and SUR2B (Cat# SC118754) cDNA clones used for heterologous expression in HEK293 cells, enabling the functional analysis of KATP channel activation mechanisms.
FigureFigure
Showing 1-1 of 1 papers.
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