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CLK1 (NM_004071) Human Tagged ORF Clone

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SKU
RC204443
CLK1 (Myc-DDK-tagged)-Human CDC-like kinase 1 (CLK1), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $457.00
In Stock*
Bulk Requests & Clone Modifications

Frequently Bought Together (6)

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PRIMER Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
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PS100001 pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
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AR100028M DDKTrap Anti-DDK 8G6 affinity resin used for the purification of DDK, DYKDDDDK, or FLAG® tagged proteins , 5 mL
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Other products for "CLK1 or NM_004071"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol CLK1
Synonyms CLK; CLK/STY; STY
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC204443 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGACACTCAAAGAGAACTTACTGTCCTGATTGGGATGACAAGGATTGGGATTATGGAAAATGGAGGA
GCAGCAGCAGTCATAAAAGAAGGAAGAGATCACATAGCAGTGCCCAGGAGAACAAGCGCTGCAAATACAA
TCACTCTAAAATGTGTGATAGCCATTATTTGGAAAGCAGGTCTATAAATGAGAAAGATTATCATAGTCGA
CGCTACATTGATGAGTACAGAAATGACTACACTCAAGGATGTGAACCTGGACATCGCCAAAGAGACCATG
AAAGCCGGTATCAGAACCATAGTAGCAAGTCTTCTGGTAGAAGTGGAAGAAGTAGTTATAAAAGCAAACA
CAGGATTCACCACAGTACTTCACATCGTCGTTCACATGGGAAGAGTCACCGAAGGAAAAGAACCAGGAGT
GTAGAGGATGATGAGGAGGGTCACCTGATCTGTCAGAGTGGAGACGTACTAAGTGCAAGATATGAAATTG
TTGATACTTTAGGTGAAGGAGCTTTTGGAAAAGTTGTGGAGTGCATCGATCATAAAGCGGGAGGTAGACA
TGTAGCAGTAAAAATAGTTAAAAATGTGGATAGATACTGTGAAGCTGCTCGCTCAGAAATACAAGTTCTG
GAACATCTGAATACAACAGACCCCAACAGTACTTTCCGCTGTGTCCAGATGTTGGAATGGTTTGAGCATC
ATGGTCACATTTGCATTGTTTTTGAACTATTGGGACTTAGTACTTACGACTTCATTAAAGAAAATGGTTT
TCTACCATTTCGACTGGATCATATCAGAAAGATGGCATATCAGATATGCAAGTCTGTGAATTTTTTGCAC
AGTAATAAGTTGACTCACACAGACTTAAAGCCTGAAAACATCTTATTTGTGCAGTCTGACTACACAGAGG
CGTATAATCCCAAAATAAAACGTGATGAACGCACCTTAATAAATCCAGATATTAAAGTTGTAGACTTTGG
TAGTGCAACATATGATGACGAACATCACAGTACATTGGTATCTACAAGACATTATAGAGCACCTGAAGTT
ATTTTAGCCCTAGGGTGGTCCCAACCATGTGATGTCTGGAGCATAGGATGCATTCTTATTGAATACTATC
TTGGGTTTACCGTATTTCCAACACACGATAGTAAGGAGCATTTAGCAATGATGGAAAGGATTCTTGGACC
TCTACCAAAACATATGATACAGAAAACCAGGAAACGTAAATATTTTCACCACGATCGATTAGACTGGGAT
GAACACAGTTCTGCCGGCAGATATGTTTCAAGACGCTGTAAACCTCTGAAGGAATTTATGCTTTCTCAAG
ATGTTGAACATGAGCGTCTCTTTGACCTCATTCAGAAAATGTTGGAGTATGATCCAGCCAAAAGAATTAC
TCTCAGAGAAGCCTTAAAGCATCCTTTCTTTGACCTTCTGAAGAAAAGTATA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC204443 protein sequence
Red=Cloning site Green=Tags(s)

MRHSKRTYCPDWDDKDWDYGKWRSSSSHKRRKRSHSSAQENKRCKYNHSKMCDSHYLESRSINEKDYHSR
RYIDEYRNDYTQGCEPGHRQRDHESRYQNHSSKSSGRSGRSSYKSKHRIHHSTSHRRSHGKSHRRKRTRS
VEDDEEGHLICQSGDVLSARYEIVDTLGEGAFGKVVECIDHKAGGRHVAVKIVKNVDRYCEAARSEIQVL
EHLNTTDPNSTFRCVQMLEWFEHHGHICIVFELLGLSTYDFIKENGFLPFRLDHIRKMAYQICKSVNFLH
SNKLTHTDLKPENILFVQSDYTEAYNPKIKRDERTLINPDIKVVDFGSATYDDEHHSTLVSTRHYRAPEV
ILALGWSQPCDVWSIGCILIEYYLGFTVFPTHDSKEHLAMMERILGPLPKHMIQKTRKRKYFHHDRLDWD
EHSSAGRYVSRRCKPLKEFMLSQDVEHERLFDLIQKMLEYDPAKRITLREALKHPFFDLLKKSI

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_004071
ORF Size 1452 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_004071.4
RefSeq Size 1933 bp
RefSeq ORF 1455 bp
Locus ID 1195
UniProt ID P49759
Cytogenetics 2q33.1
Domains pkinase, S_TKc, TyrKc
Protein Families Druggable Genome, Protein Kinase
MW 57.3 kDa
Summary This gene encodes a member of the CDC2-like (or LAMMER) family of dual specificity protein kinases. In the nucleus, the encoded protein phosphorylates serine/arginine-rich proteins involved in pre-mRNA processing, releasing them into the nucleoplasm. The choice of splice sites during pre-mRNA processing may be regulated by the concentration of transacting factors, including serine/arginine rich proteins. Therefore, the encoded protein may play an indirect role in governing splice site selection. Multiple transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Jun 2009
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Documents
Resources
"NM_004071" in other vectors (9)
SKU Description Size Price
RC204443L1 Lenti ORF clone of Human CDC-like kinase 1 (CLK1), transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC204443L2 Lenti ORF clone of Human CDC-like kinase 1 (CLK1), transcript variant 1, mGFP tagged 10 ug
$757.00
RC204443L3 Lenti ORF clone of Human CDC-like kinase 1 (CLK1), transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC204443L4 Lenti ORF clone of Human CDC-like kinase 1 (CLK1), transcript variant 1, mGFP tagged 10 ug
$757.00
RG204443 CLK1 (tGFP-tagged) - Human CDC-like kinase 1 (CLK1), transcript variant 1 10 ug
$489.00 $657.00
SC117574 CLK1 (untagged)-Human CDC-like kinase 1 (CLK1), transcript variant 1 10 ug
$457.00
SC323440 CLK1 (untagged)-Kinase deficient mutant (K191M) of Human CDC-like kinase 1 (CLK1) 10 ug
$457.00
SC323558 CLK1 (untagged)-Kinase deficient mutant (K191M) of Human CDC-like kinase 1 (CLK1) 10 ug
$457.00
SC324172 CLK1 (untagged)-Human CDC-like kinase 1 (CLK1), transcript variant 1 10 ug
$457.00

Plasmid Map for RC204443

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC204443
Circular map for RC204443

file-alt Citations

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