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TMEM143 (NM_018273) Human Tagged ORF Clone

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SKU
RC204409
TMEM143 (Myc-DDK-tagged)-Human transmembrane protein 143 (TMEM143)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol TMEM143
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC204409 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACAGTCGAGCTTTGGCTAAGGCTCCGGGGAAAGGGTCTAGCCATGCTGCATGTGACCCGGGGGGTCT
GGGGGTCCAGGGTCCGAGTATGGCCACTGTTGCCCGCGCTCCTCGGGCCCCCCCGGGCCCTCTCATCGCT
GGCAGCCAAAATGGGGGAGTATCGCAAGATGTGGAACCCCAGGGAGCCCCGCGACTGGGCCCAGCAGTAC
CGCGAGCGCTTCATTCCCTTCTCCAAGGAGCAGCTGCTCCGCCTCCTAATACAGGAATTCCACTCGAGTC
CGGCAGAGAAGGCGGCTTTGGAGGCGTTCTCGGCCCACGTGGACTTCTGCACCCTGTTCCACTACCACCA
AATCCTGGCCCGGCTGCAGGCCTTATATGACCCCATCAACCCTGACAGGGAGACCCTCGATCAGCCATCA
CTAACGGATCCCCAGCGTCTGTCTAATGAGCAGGAGGTGCTTCGGGCTCTGGAGCCCCTGCTGGCCCAGG
CCAACTTCTCCCCGCTGTCTGAGGACACCCTGGCCTACGCGCTGGTGGTCCACCACCCTCAGGATGAGGT
CCAGGTGACAGTAAATTTGGATCAGTATGTCTACATTCACTTCTGGGCCCTGGGCCAGCGAGTCGGGCAG
ATGCCCCTGAAGTCCAGCGTGGGCTCCAGGCGTGGCTTCTTCACCAAGCTGCCCCCTGCGGAGAGGAGAT
ACTTTAAGCGGGTGGTCCTGGCAGCCCGGACCAAACGGGGACACCTGGTACTGAAGAGTTTCAAGGACAC
GCCGCTGGAAGGCCTGGAGCAGCTGCTGCCGGAGCTGAAGGTGCGCACGCCCACCCTGCAGCGCGCCCTG
CTCAACCTCATGCTGGTAGTCTCCGGCGTGGCGATCTTCGTCAACGTGGGCATGGTGGTGCTAACCGACC
TCAAGGTGGCCACCTCCCTGCTGCTGCTGCTCTTCGCCATCTTCATGGGCCTGCGGGCCTCCAAGATGTT
CGGGCAGCGGCGCAGCGCGCAGGCGTTGGAGCTGGCGCACATGCTGTACTATCGCAGTACGTCCAACAAC
TCGGAGCTGCTCAGCGCCCTGGCCCTGCGCGCGCAGGACGAGCACACCAAGGAGGCGCTGCTGGCTCACA
GCTTCCTGGCCCGGCGGCCAGGGGGCACTCAAGGCTCGCCCGAAGAGACCTCCAGGTGGCTCAGGTCGGA
GGTGGAGAACTGGCTCCTAGCCAAGTCAGGCTGTGAGGTGACCTTCAACGGAACTCGGGCCCTGGCGCAT
CTGCAGGCCCTGACCCCCAGCATGGGATTGTACCCACCCCCGGGTTTCCCCAAACTAGACCCAGTGGCTC
CGATCACTTCCGAGCCCCCGCAAGCCACGCCCAGCAGTAACATCTCC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC204409 protein sequence
Red=Cloning site Green=Tags(s)

MTVELWLRLRGKGLAMLHVTRGVWGSRVRVWPLLPALLGPPRALSSLAAKMGEYRKMWNPREPRDWAQQY
RERFIPFSKEQLLRLLIQEFHSSPAEKAALEAFSAHVDFCTLFHYHQILARLQALYDPINPDRETLDQPS
LTDPQRLSNEQEVLRALEPLLAQANFSPLSEDTLAYALVVHHPQDEVQVTVNLDQYVYIHFWALGQRVGQ
MPLKSSVGSRRGFFTKLPPAERRYFKRVVLAARTKRGHLVLKSFKDTPLEGLEQLLPELKVRTPTLQRAL
LNLMLVVSGVAIFVNVGMVVLTDLKVATSLLLLLFAIFMGLRASKMFGQRRSAQALELAHMLYYRSTSNN
SELLSALALRAQDEHTKEALLAHSFLARRPGGTQGSPEETSRWLRSEVENWLLAKSGCEVTFNGTRALAH
LQALTPSMGLYPPPGFPKLDPVAPITSEPPQATPSSNIS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_018273
ORF Size 1377 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_018273.2, NP_060743.2
RefSeq Size 2265 bp
RefSeq ORF 1380 bp
Locus ID 55260
UniProt ID Q96AN5
Cytogenetics 19q13.33
Protein Families Transmembrane
MW 51.7 kDa
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Documents
Resources
"NM_018273" in other vectors (4)
SKU Description Size Price
RC204409L3 Lenti ORF clone of Human transmembrane protein 143 (TMEM143), Myc-DDK-tagged 10 ug
$757.00
RC204409L4 Lenti ORF clone of Human transmembrane protein 143 (TMEM143), mGFP tagged 10 ug
$757.00
RG204409 TMEM143 (tGFP-tagged) - Human transmembrane protein 143 (TMEM143) 10 ug
$489.00 $657.00
SC324664 TMEM143 (untagged)-Human transmembrane protein 143 (TMEM143) 10 ug
$457.00

Plasmid Map for RC204409

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC204409
Circular map for RC204409

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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