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Nucleotide binding protein like (NUBPL) (NM_025152) Human Tagged ORF Clone

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SKU
RC204385
NUBPL (Myc-DDK-tagged)-Human nucleotide binding protein-like (NUBPL), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol Nucleotide binding protein like
Synonyms C14orf127; huInd1; IND1; MC1DN21
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC204385 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTTTGTGGGCGCCAGTTGTCTGGCGCCGGGAGTGAGACCCTAAAACAAAGAAGAACACAAATCATGT
CCCGAGGACTTCCAAAGCAGAAACCGATAGAAGGTGTTAAACAAGTTATAGTTGTGGCTTCTGGAAAGGG
TGGAGTCGGAAAATCTACTACAGCAGTGAATCTTGCACTTGCACTAGCAGCGAACGATTCGTCCAAGGCC
ATTGGTTTGCTAGATGTGGATGTGTATGGACCTTCAGTTCCAAAGATGATGAATCTGAAAGGAAATCCGG
AATTATCACAGAGCAACCTAATGAGGCCTCTCTTGAATTATGGTATTGCTTGTATGTCTATGGGCTTTCT
GGTTGAAGAAAGTGAACCAGTAGTTTGGAGAGGCCTTATGGTAATGTCGGCCATTGAGAAATTGTTGAGG
CAGGTAGATTGGGGTCAACTGGACTACTTAGTTGTAGACATGCCACCAGGAACTGGAGATGTGCAGTTAT
CAGTCTCACAGACTATTCCTATAACAGGTGCTGTGATTGTCTCCACGCCCCAGGACATCGCATTGATGGA
TGCACACAAGGGTGCTGAGATGTTTCGCAGAGTCCACGTGCCCGTCCTTGGCCTTGTCCAAAACATGAGT
GTTTTCCAGTGTCCAAAATGTAAACACAAAACTCATATTTTTGGTGCTGATGGTGCAAGGAAACTAGCAC
AGACCCTTGGTCTTGAAGTTCTAGGAGACATTCCCTTACACCTTAATATAAGGGAAGCTTCAGATACAGG
CCAGCCAATTGTGTTTTCACAGCCTGAAAGTGATGAGGCCAAAGCTTACTTGAGGATTGCTGTGGAAGTG
GTAAGAAGATTGCCATCACCTTCAGAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC204385 protein sequence
Red=Cloning site Green=Tags(s)

MVCGRQLSGAGSETLKQRRTQIMSRGLPKQKPIEGVKQVIVVASGKGGVGKSTTAVNLALALAANDSSKA
IGLLDVDVYGPSVPKMMNLKGNPELSQSNLMRPLLNYGIACMSMGFLVEESEPVVWRGLMVMSAIEKLLR
QVDWGQLDYLVVDMPPGTGDVQLSVSQTIPITGAVIVSTPQDIALMDAHKGAEMFRRVHVPVLGLVQNMS
VFQCPKCKHKTHIFGADGARKLAQTLGLEVLGDIPLHLNIREASDTGQPIVFSQPESDEAKAYLRIAVEV
VRRLPSPSE

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_025152
ORF Size 867 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_025152.1, NP_079428.1
RefSeq Size 3072 bp
RefSeq ORF 960 bp
Locus ID 80224
UniProt ID Q8TB37, X5D2R5
Cytogenetics 14q12
MW 31.1 kDa
Summary This gene encodes a member of the Mrp/NBP35 ATP-binding proteins family. The encoded protein is required for the assembly of the respiratory chain NADH dehydrogenase (complex I), an oligomeric enzymatic complex located in the inner mitochondrial membrane. Mutations in this gene cause mitochondrial complex I deficiency. Alternative splicing results in multiple transcript variants. provided by RefSeq, May 2014
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"NM_025152" in other vectors (5)
SKU Description Size Price
RC204385L3 Lenti ORF clone of Human nucleotide binding protein-like (NUBPL), transcript variant 1, Myc-DDK-tagged 10 ug
$600.00
RC204385L4 Lenti ORF clone of Human nucleotide binding protein-like (NUBPL), transcript variant 1, mGFP tagged 10 ug
$600.00
RG204385 NUBPL (tGFP-tagged) - Human nucleotide binding protein-like (NUBPL), transcript variant 1 10 ug
$489.00 $500.00
SC317484 NUBPL (untagged)-Human nucleotide binding protein-like (NUBPL), transcript variant 1 10 ug
$330.00
SC321763 NUBPL (untagged)-Human nucleotide binding protein-like (NUBPL), transcript variant 1 10 ug
$300.00

Plasmid Map for RC204385

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC204385
Circular map for RC204385

file-alt Citations

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