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NUDT18 (NM_024815) Human Tagged ORF Clone

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SKU
RC204376
NUDT18 (Myc-DDK-tagged)-Human nudix (nucleoside diphosphate linked moiety X)-type motif 18 (NUDT18)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol NUDT18
Synonyms MTH3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC204376 representing NM_024815
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCTCGGAGGGCCTGGCGGGGGCGCTGGCTTCCGTGCTGGCTGGCCAGGGGTCCAGCGTGCACAGCT
GCGACTCGGCGCCGGCCGGGGAGCCGCCGGCGCCCGTGCGGCTGCGGAAGAACGTGTGCTACGTGGTGCT
GGCCGTGTTCCTCAGCGAGCAGGATGAGGTGCTACTGATCCAGGAGGCCAAGAGGGAGTGCCGGGGGTCG
TGGTACCTGCCTGCGGGGAGAATGGAGCCAGGGGAGACCATCGTGGAGGCGCTGCAGCGGGAGGTGAAGG
AGGAGGCGGGGCTGCACTGTGAGCCCGAGACACTGCTGTCCGTGGAGGAGCGGGGCCCCTCCTGGGTCCG
CTTCGTGTTCCTCGCTCGCCCCACAGGTGGAATTCTCAAGACTTCCAAGGAGGCCGATGCGGAGTCCCTG
CAGGCTGCCTGGTACCCACGGACCTCCCTGCCCACTCCGCTGCGAGCCCATGACATCCTGCACCTGGTTG
AACTAGCCGCCCAGTATCGCCAGCAAGCCAGGCACCCTCTCATTCTGCCCCAAGAGCTACCCTGTGATCT
GGTCTGCCAGCGGCTCGTGGCTACCTTTACCAGCGCCCAGACAGTGTGGGTGTTAGTGGGCACAGTGGGG
ATGCCTCACTTGCCTGTCACTGCCTGTGGCCTCGACCCTATGGAGCAGAGGGGTGGCATGAAGATGGCCG
TCCTGCGGCTGCTGCAGGAGTGTCTGACCCTGCACCACTTGGTGGTGGAGATCAAGGGGTTGCTTGGACT
GCAGCACCTGGGCCGAGATCACAGTGATGGCATCTGTTTGAATGTGCTGGTGACCGTGGCTTTTCGGAGC
CCAGGGATCCAGGATGAACCCCCAAAAGTTCGGGGTGAGAACTTCTCTTGGTGGAAGGTGATGGAGGAAG
ACCTGCAAAGCCAGCTCCTCCAGCGGCTTCAGGGATCCTCTGTTGTCCCAGTGAACAGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC204376 representing NM_024815
Red=Cloning site Green=Tags(s)

MASEGLAGALASVLAGQGSSVHSCDSAPAGEPPAPVRLRKNVCYVVLAVFLSEQDEVLLIQEAKRECRGS
WYLPAGRMEPGETIVEALQREVKEEAGLHCEPETLLSVEERGPSWVRFVFLARPTGGILKTSKEADAESL
QAAWYPRTSLPTPLRAHDILHLVELAAQYRQQARHPLILPQELPCDLVCQRLVATFTSAQTVWVLVGTVG
MPHLPVTACGLDPMEQRGGMKMAVLRLLQECLTLHHLVVEIKGLLGLQHLGRDHSDGICLNVLVTVAFRS
PGIQDEPPKVRGENFSWWKVMEEDLQSQLLQRLQGSSVVPVNR

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_024815
ORF Size 969 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_024815.4
RefSeq Size 1519 bp
RefSeq ORF 972 bp
Locus ID 79873
UniProt ID Q6ZVK8
Cytogenetics 8p21.3
MW 35.3 kDa
Summary The protein encoded by this gene is a member of the Nudix hydrolase family. Nudix hydrolases eliminate potentially toxic nucleotide metabolites from the cell and regulate the concentrations and availability of many different nucleotide substrates, cofactors, and signaling molecules. This protein contains a Nudix hydrolase domain and hydrolyzes oxidized forms of guanosine and deoxyguanosine diphosphates. provided by RefSeq, Sep 2012
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"NM_024815" in other vectors (6)
SKU Description Size Price
RC204376L1 Lenti ORF clone of Human nudix (nucleoside diphosphate linked moiety X)-type motif 18 (NUDT18), Myc-DDK-tagged 10 ug
$600.00
RC204376L2 Lenti ORF clone of Human nudix (nucleoside diphosphate linked moiety X)-type motif 18 (NUDT18), mGFP tagged 10 ug
$600.00
RC204376L3 Lenti ORF clone of Human nudix (nucleoside diphosphate linked moiety X)-type motif 18 (NUDT18), Myc-DDK-tagged 10 ug
$600.00
RC204376L4 Lenti ORF clone of Human nudix (nucleoside diphosphate linked moiety X)-type motif 18 (NUDT18), mGFP tagged 10 ug
$600.00
RG204376 NUDT18 (tGFP-tagged) - Human nudix (nucleoside diphosphate linked moiety X)-type motif 18 (NUDT18) 10 ug
$489.00 $500.00
SC322527 NUDT18 (untagged)-Human nudix (nucleoside diphosphate linked moiety X)-type motif 18 (NUDT18) 10 ug
$300.00

Plasmid Map for RC204376

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC204376
Circular map for RC204376

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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