TIFA (NM_052864) Human Tagged Lenti ORF Clone

  • LentiORF®
SKU
RC204357L4
Lenti ORF clone of Human TRAF-interacting protein with forkhead-associated domain (TIFA), mGFP tagged
  $600.00
3 Weeks*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol TIFA
Synonyms T2BP; T6BP; TIFAA
Vector pLenti-C-mGFP-P2A-Puro
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection Puromycin
Sequence Data
ORF Nucleotide Sequence
The ORF insert of this clone is exactly the same as(RC204357).
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_052864
ORF Size 552 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_052864.2
RefSeq Size 3090 bp
RefSeq ORF 555 bp
Locus ID 92610
UniProt ID Q96CG3
Cytogenetics 4q25
MW 21.4 kDa
Summary This gene encodes an adapter protein involved in adaptive and innate immunity. This protein includes a forkhead-associated (FHA) domain that specifically binds to phosphorylated serine and threonine residues. In response to bacterial infection, the encoded host cell protein undergoes an intermolecular interaction between the FHA domain and a phosphorylated threonine that leads to protein oligomerization and stimulation of the NF-kappa B and other downstream signaling pathways. This protein exhibits reduced expression in hepatocellular carcinoma and may suppress hepatocellular carcinoma progression. This protein may also play a role in the DNA damage response. provided by RefSeq, Jun 2018
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Documents
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"NM_052864" in other vectors (4)
SKU Description Size Price
RC204357 TIFA (Myc-DDK-tagged)-Human TRAF-interacting protein with forkhead-associated domain (TIFA) 10 ug
$300.00
RC204357L3 Lenti ORF clone of Human TRAF-interacting protein with forkhead-associated domain (TIFA), Myc-DDK-tagged 10 ug
$600.00
RG204357 TIFA (tGFP-tagged) - Human TRAF-interacting protein with forkhead-associated domain (TIFA) 10 ug
$489.00 $500.00
SC107567 TIFA (untagged)-Human TRAF-interacting protein with forkhead-associated domain (TIFA) 10 ug
$300.00

Plasmid Map for RC204357L4

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Cloning scheme for RC204357L4

Circular map for RC204357L4

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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