Syntaxin 16 (STX16) (NM_003763) Human Tagged ORF Clone

SKU RC204347

STX16 (Myc-DDK-tagged)-Human syntaxin 16 (STX16), transcript variant 2

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: Syntaxin 16
• Synonyms: SYN16
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC204347 ORF sequence; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGCCACCAGGCGTTTAACCGACGCTTTCTTGTTGTTGCGGAATAATTCCATCCAAAACCGGCAGCTGT; TAGCCGAGCAACTTGCTGATGACCGTATGGCACTGGTGTCAGGCATCAGCTTAGATCCAGAAGCAGCGAT; TGGTGTGACAAAACGGCCACCTCCTAAGTGGGTGGATGGAGTGGATGAAATTCAGTATGATGTTGGCCGG; ATTAAGCAGAAGATGAAAGAATTGGCCAGCCTTCATGACAAGCATTTAAACAGACCCACCCTGGATGACA; GCAGCGAAGAGGAACATGCCATTGAGATAACTACCCAAGAGATCACTCAGCTCTTCCACAGGTGCCAGCG; TGCCGTGCAGGCCCTGCCGAGCCGGGCCCGGGCCTGCTCCGAGCAGGAGGGGCGGCTGCTTGGGAACGTG; GTGGCCTCGCTGGCGCAGGCCCTGCAGGAACTCTCCACCAGCTTCCGGCACGCACAGTCAGGCTACCTCA; AACGCATGAAGAATCGAGAGGAAAGATCCCAGCATTTTTTCGACACATCAGTACCACTAATGGATGATGG; AGACGATAACACTCTTTACCATCGGGGTTTTACAGAGGACCAGTTAGTTCTGGTGGAGCAGAACACACTG; ATGGTGGAAGAGCGGGAACGAGAGATTCGCCAGATTGTACAGTCCATTTCTGACCTGAATGAAATATTCA; GGGACTTAGGGGCGATGATTGTAGAACAGGGTACAGTCCTTGACAGAATTGACTATAACGTTGAACAGTC; CTGTATCAAAACTGAAGATGGTTTGAAACAGCTTCACAAGGCAGAACAGTATCAAAAGAAGAATCGGAAG; ATGCTTGTGATTTTAATATTATTTGTCATCATCATTGTGCTCATTGTTGTCCTCGTTGGCGTGAAGTCTC; GA; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC204347 protein sequence; Red=Cloning site Green=Tags(s); ; MATRRLTDAFLLLRNNSIQNRQLLAEQLADDRMALVSGISLDPEAAIGVTKRPPPKWVDGVDEIQYDVGR; IKQKMKELASLHDKHLNRPTLDDSSEEEHAIEITTQEITQLFHRCQRAVQALPSRARACSEQEGRLLGNV; VASLAQALQELSTSFRHAQSGYLKRMKNREERSQHFFDTSVPLMDDGDDNTLYHRGFTEDQLVLVEQNTL; MVEEREREIRQIVQSISDLNEIFRDLGAMIVEQGTVLDRIDYNVEQSCIKTEDGLKQLHKAEQYQKKNRK; MLVILILFVIIIVLIVVLVGVKSR; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003763
• ORF Size: 912 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_003763.5
• RefSeq Size: 4915 bp
• RefSeq ORF: 915 bp
• Locus ID: 8675
• UniProt ID: O14662
• Cytogenetics: 20q13.32
• Domains: SynN, t_SNARE
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: SNARE interactions in vesicular transport
• MW: 34.8 kDa
• Summary: This gene encodes a protein that is a member of the syntaxin or t-SNARE (target-SNAP receptor) family. These proteins are found on cell membranes and serve as the targets for V-SNARES (vesicle-SNAP receptors) permitting specific synaptic vesicle docking and fusion. A microdeletion in the region of chromosome 20 where this gene is located has been associated with pseudohypoparathyroidism type Ib. Multiple transcript variants have been found for this gene. Read-through transcription also exists between this gene and the neighboring downstream aminopeptidase-like 1 (NPEPL1) gene. [provided by RefSeq, Mar 2011]