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MRPL53 (NM_053050) Human Tagged ORF Clone

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SKU
RC204255
MRPL53 (Myc-DDK-tagged)-Human mitochondrial ribosomal protein L53 (MRPL53), nuclear gene encoding mitochondrial protein
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $150.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol MRPL53
Synonyms L53MT
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC204255 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAGCTGCCTTGGCTCGGCTTGGTCTGCGGCCTGTCAAACAGGTTCGGGTTCAGTTCTGTCCCTTCG
AGAAAAACGTGGAATCGACGAGGACCTTCCTGCAGACGGTGAGCAGTGAGAAGGTCCGCTCCACTAATCT
CAACTGCTCAGTGATTGCGGACGTGAGGCATGACGGCTCCGAGCCCTGCGTGGACGTGCTGTTCGGAGAC
GGGCATCGCCTGATTATGCGCGGCGCTCATCTCACCGCTCTGGAAATGCTCACCGCCTTCGCCTCCCACA
TCCGGGCCAGGGACGCGGCGGGCAGCGGGGACAAGCCGGGCGCTGATACTGGTCGC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC204255 protein sequence
Red=Cloning site Green=Tags(s)

MAAALARLGLRPVKQVRVQFCPFEKNVESTRTFLQTVSSEKVRSTNLNCSVIADVRHDGSEPCVDVLFGD
GHRLIMRGAHLTALEMLTAFASHIRARDAAGSGDKPGADTGR

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_053050
ORF Size 336 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_053050.5
RefSeq Size 673 bp
RefSeq ORF 339 bp
Locus ID 116540
UniProt ID Q96EL3
Cytogenetics 2p13.1
MW 12.1 kDa
Summary Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. A pseudogene corresponding to this gene is found on chromosome 1p. provided by RefSeq, Jul 2008
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"NM_053050" in other vectors (6)
SKU Description Size Price
RC204255L1 Lenti ORF clone of Human mitochondrial ribosomal protein L53 (MRPL53), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged 10 ug
$450.00
RC204255L2 Lenti ORF clone of Human mitochondrial ribosomal protein L53 (MRPL53), nuclear gene encoding mitochondrial protein, mGFP tagged 10 ug
$450.00
RC204255L3 Lenti ORF clone of Human mitochondrial ribosomal protein L53 (MRPL53), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged 10 ug
$450.00
RC204255L4 Lenti ORF clone of Human mitochondrial ribosomal protein L53 (MRPL53), nuclear gene encoding mitochondrial protein, mGFP tagged 10 ug
$450.00
RG204255 MRPL53 (tGFP-tagged) - Human mitochondrial ribosomal protein L53 (MRPL53), nuclear gene encoding mitochondrial protein 10 ug
$489.00
SC120204 MRPL53 (untagged)-Human mitochondrial ribosomal protein L53 (MRPL53), nuclear gene encoding mitochondrial protein 10 ug
$150.00

Plasmid Map for RC204255

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC204255
Circular map for RC204255

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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