WDFY2 (NM_052950) Human Tagged ORF Clone

  • TrueORF®
SKU
RC204225
WDFY2 (Myc-DDK-tagged)-Human WD repeat and FYVE domain containing 2 (WDFY2)
$289.00 $457.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol WDFY2
Synonyms PROF; WDF2; ZFYVE22
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC204225 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGCGGAGATCCAGCCCAAGCCTCTGACCCGCAAGCCGATCCTGCTGCAGCGGATGGAGGGGTCCC
AGGAGGTGGTGAATATGGCCGTGATCGTGCCCAAAGAGGAGGGCGTCATCAGCGTCTCCGAGGACAGGAC
AGTTCGTGTTTGGTTAAAGAGAGACAGTGGACAGTATTGGCCAAGCGTATACCATGCAATGCCTTCTCCA
TGTTCATGCATGTCTTTTAACCCGGAAACAAGAAGACTGTCCATAGGTCTAGACAATGGTACAATCTCAG
AGTTTATATTGTCAGAAGATTATAACAAGATGACTCCTGTGAAAAACTATCAAGCGCATCAGAGCAGAGT
GACGATGATCCTGTTTGTCCTGGAGCTGGAGTGGGTGCTGAGCACAGGACAGGACAAGCAATTTGCCTGG
CACTGCTCTGAGAGTGGGCAGCGCCTGGGAGGTTATCGGACCAGTGCTGTGGCCTCAGGCCTGCAATTTG
ATGTTGAAACCCGGCATGTGTTTATCGGTGACCACTCAGGCCAAGTAACAATCCTCAAACTGGAGCAAGA
AAACTGCACCCTGGTCACAACATTCAGAGGACACACAGGTGGGGTGACCGCTCTCTGTTGGGACCCAGTC
CAGCGGGTGTTGTTCTCAGGCAGTTCAGATCACTCTGTCATCATGTGGGACATCGGTGGGAGAAAAGGAA
CAGCCATCGAGCTCCAAGGACACAACGACAGAGTCCAGGCCCTCTCCTATGCACAGCACACGCGACAATT
GATCTCCTGTGGCGGTGATGGTGGGATTGTCGTCTGGAACATGGACGTGGAGAGGCAGGAGACCCCTGAA
TGGTTGGACAGTGATTCCTGCCAAAAGTGTGATCAGCCTTTCTTCTGGAACTTCAAGCAAATGTGGGACA
GTAAGAAAATTGGTCTAAGACAGCACCACTGCCGCAAGTGTGGGAAGGCCGTCTGTGGCAAGTGCAGCTC
CAAGCGCTCCTCCATCCCCCTGATGGGCTTCGAGTTTGAAGTGAGGGTCTGTGACAGCTGCCACGAGGCC
ATCACAGATGAAGAACGTGCACCCACAGCCACCTTCCATGACAGTAAACATAACATTGTGCATGTGCATT
TCGATGCAACCAGAGGATGGTTACTGACTTCTGGAACTGACAAGGTTATTAAGTTGTGGGATATGACCCC
AGTCGTGTCT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC204225 protein sequence
Red=Cloning site Green=Tags(s)

MAAEIQPKPLTRKPILLQRMEGSQEVVNMAVIVPKEEGVISVSEDRTVRVWLKRDSGQYWPSVYHAMPSP
CSCMSFNPETRRLSIGLDNGTISEFILSEDYNKMTPVKNYQAHQSRVTMILFVLELEWVLSTGQDKQFAW
HCSESGQRLGGYRTSAVASGLQFDVETRHVFIGDHSGQVTILKLEQENCTLVTTFRGHTGGVTALCWDPV
QRVLFSGSSDHSVIMWDIGGRKGTAIELQGHNDRVQALSYAQHTRQLISCGGDGGIVVWNMDVERQETPE
WLDSDSCQKCDQPFFWNFKQMWDSKKIGLRQHHCRKCGKAVCGKCSSKRSSIPLMGFEFEVRVCDSCHEA
ITDEERAPTATFHDSKHNIVHVHFDATRGWLLTSGTDKVIKLWDMTPVVS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_052950
ORF Size 1200 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_052950.4
RefSeq Size 3809 bp
RefSeq ORF 1203 bp
Locus ID 115825
UniProt ID Q96P53
Cytogenetics 13q14.3
Domains FYVE, WD40
MW 45.2 kDa
Summary This gene encodes a protein that contains two WD domains and an FYVE zinc finger region. The function of this gene is unknown. An alternatively spliced transcript variant of this gene may exist. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_052950" in other vectors (6)
SKU Description Size Price
RC204225L1 Lenti ORF clone of Human WD repeat and FYVE domain containing 2 (WDFY2), Myc-DDK-tagged 10 ug
$757.00
RC204225L2 Lenti ORF clone of Human WD repeat and FYVE domain containing 2 (WDFY2), mGFP tagged 10 ug
$757.00
RC204225L3 Lenti ORF clone of Human WD repeat and FYVE domain containing 2 (WDFY2), Myc-DDK-tagged 10 ug
$757.00
RC204225L4 Lenti ORF clone of Human WD repeat and FYVE domain containing 2 (WDFY2), mGFP tagged 10 ug
$757.00
RG204225 WDFY2 (tGFP-tagged) - Human WD repeat and FYVE domain containing 2 (WDFY2) 10 ug
$489.00 $657.00
SC321620 WDFY2 (untagged)-Human WD repeat and FYVE domain containing 2 (WDFY2) 10 ug
$457.00

Plasmid Map for RC204225

Download sequence for

Cloning scheme for RC204225

Circular map for RC204225

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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