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STK38 (NM_007271) Human Tagged ORF Clone

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SKU
RC204133
STK38 (Myc-DDK-tagged)-Human serine/threonine kinase 38 (STK38)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol STK38
Synonyms NDR; NDR1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC204133 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAATGACAGGCTCAACACCTTGCTCATCCATGAGTAACCACACAAAGGAAAGGGTGACAATGACCA
AAGTGACACTGGAGAATTTTTATAGCAACCTTATCGCTCAACATGAAGAACGAGAAATGAGACAAAAGAA
GTTAGAAAAGGTGATGGAAGAAGAAGGCCTAAAAGATGAGGAGAAACGACTCCGGAGATCAGCACATGCT
CGGAAGGAAACAGAGTTTCTTCGTTTGAAGAGAACAAGACTTGGATTGGAAGATTTTGAGTCCTTAAAAG
TAATAGGCAGAGGAGCATTTGGTGAGGTACGGCTTGTTCAGAAGAAAGATACGGGACATGTGTATGCAAT
GAAAATACTCCGTAAAGCAGATATGCTTGAAAAAGAGCAGGTTGGCCACATTCGTGCGGAGCGTGACATT
CTAGTGGAGGCAGACAGTTTGTGGGTTGTGAAAATGTTCTATAGTTTTCAGGATAAGCTAAACCTCTACC
TAATCATGGAGTTCCTGCCTGGAGGGGACATGATGACCTTGTTGATGAAAAAAGACACTCTGACAGAAGA
GGAGACTCAGTTTTATATAGCAGAAACAGTATTAGCCATAGACTCTATTCACCAACTTGGATTCATCCAC
AGAGACATCAAACCAGACAACCTTCTTTTGGACAGCAAGGGCCATGTGAAACTTTCTGACTTTGGTCTTT
GCACAGGACTGAAAAAAGCACATAGGACAGAATTTTATAGGAATCTGAACCACAGCCTCCCCAGTGATTT
CACTTTCCAGAACATGAATTCCAAAAGGAAAGCAGAAACCTGGAAAAGAAATAGACGTCAGCTAGCCTTC
TCCACAGTAGGCACTCCTGACTACATTGCTCCTGAGGTGTTCATGCAGACCGGGTACAACAAGCTCTGTG
ATTGGTGGTCGCTTGGGGTGATCATGTATGAGATGCTCATCGGCTACCCACCTTTCTGTTCTGAGACCCC
TCAAGAGACATATAAGAAGGTGATGAACTGGAAAGAAACTTTGACTTTTCCTCCAGAAGTTCCCATCTCT
GAGAAAGCCAAGGATCTAATTTTGAGGTTCTGCTGTGAATGGGAACATAGAATTGGAGCTCCTGGAGTTG
AGGAAATAAAAAGTAACTCTTTTTTTGAAGGCGTTGACTGGGAACATATCAGAGAGAGACCTGCTGCAAT
ATCTATTGAAATCAAAAGCATTGATGATACCTCAAACTTCGATGAGTTTCCAGAATCTGATATTCTTAAG
CCAACAGTGGCCACAAGTAATCATCCTGAGACTGACTACAAGAACAAAGACTGGGTCTTCATCAATTACA
CGTACAAGCGCTTTGAGGGCCTGACTGCAAGGGGGGCAATACCTTCCTACATGAAAGCAGCAAAA

AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC204133 protein sequence
Red=Cloning site Green=Tags(s)

MAMTGSTPCSSMSNHTKERVTMTKVTLENFYSNLIAQHEEREMRQKKLEKVMEEEGLKDEEKRLRRSAHA
RKETEFLRLKRTRLGLEDFESLKVIGRGAFGEVRLVQKKDTGHVYAMKILRKADMLEKEQVGHIRAERDI
LVEADSLWVVKMFYSFQDKLNLYLIMEFLPGGDMMTLLMKKDTLTEEETQFYIAETVLAIDSIHQLGFIH
RDIKPDNLLLDSKGHVKLSDFGLCTGLKKAHRTEFYRNLNHSLPSDFTFQNMNSKRKAETWKRNRRQLAF
STVGTPDYIAPEVFMQTGYNKLCDWWSLGVIMYEMLIGYPPFCSETPQETYKKVMNWKETLTFPPEVPIS
EKAKDLILRFCCEWEHRIGAPGVEEIKSNSFFEGVDWEHIRERPAAISIEIKSIDDTSNFDEFPESDILK
PTVATSNHPETDYKNKDWVFINYTYKRFEGLTARGAIPSYMKAAK

SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-RsrII Cloning Scheme for this gene | Plasmid Map
ACCN NM_007271
ORF Size 1395 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_007271.4
RefSeq Size 3593 bp
RefSeq ORF 1398 bp
Locus ID 11329
UniProt ID Q15208, A0A024RD18
Cytogenetics 6p21.31
Domains pkinase, S_TKc, TyrKc
Protein Families Druggable Genome, Protein Kinase
MW 54.2 kDa
Summary This gene encodes a member of the AGC serine/threonine kinase family of proteins. The kinase activity of this protein is regulated by autophosphorylation and phosphorylation by other upstream kinases. This protein has been shown to function in the cell cycle and apoptosis. This protein has also been found to regulate the protein stability and transcriptional activity of the MYC oncogene. Alternative splicing results in multiple transcript variants. provided by RefSeq, Feb 2015
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Documents
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"NM_007271" in other vectors (7)
SKU Description Size Price
RC204133L1 Lenti ORF clone of Human serine/threonine kinase 38 (STK38), Myc-DDK-tagged 10 ug
$757.00
RC204133L2 Lenti ORF clone of Human serine/threonine kinase 38 (STK38), mGFP tagged 10 ug
$757.00
RC204133L3 Lenti ORF clone of Human serine/threonine kinase 38 (STK38), Myc-DDK-tagged 10 ug
$757.00
RC204133L4 Lenti ORF clone of Human serine/threonine kinase 38 (STK38), mGFP tagged 10 ug
$757.00
RG204133 STK38 (tGFP-tagged) - Human serine/threonine kinase 38 (STK38) 10 ug
$489.00 $657.00
SC115629 STK38 (untagged)-Human serine/threonine kinase 38 (STK38) 10 ug
$457.00
SC323502 STK38 (untagged)-Kinase deficient mutant (K118M) of Human serine/threonine kinase 38 (STK38) 10 ug
$457.00

Plasmid Map for RC204133

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC204133
Circular map for RC204133

file-alt Citations

MiR-221 negatively regulates innate anti-viral response
Du, Cui, Li et al
PLoS ONE (2018) 13 (8), e0200385 DOI: 10.1371/journal.pone.0200385
AI Summary: OriGene’s pCMV-MIR MicroRNA Expression Vector was used to clone various miRNAs, enabling functional studies that demonstrated miR-221’s role as a negative regulator in the innate antiviral response.
Snippet: Pcmv5-human ELF4 has been described previously [21]. The plasmids coding human STING, MAVS, TBK1, IRF3, IRF7 and STK38 have been described previously [25]. Various miR-RNAs were cloned into pCMV-MIR MicroRNA Expression Vector (OriGene). RNA interference Cached
FigureFigureFigure
Showing 1-1 of 1 papers.
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