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PLEKHB2 (NM_017958) Human Tagged ORF Clone

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SKU
RC204118
PLEKHB2 (Myc-DDK-tagged)-Human pleckstrin homology domain containing, family B (evectins) member 2 (PLEKHB2), transcript variant 2
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PLEKHB2
Synonyms EVT2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC204118 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGTTTGTGAAGAGTGGCTGGTTGCTGCGACAGAGTACTATTTTGAAGCGCTGGAAGAAGAACTGGT
TTGATCTGTGGTCGGATGGTCACCTGATCTATTATGATGACCAGACTCGGCAGAATATCGAGGATAAGGT
CCACATGCCAATGGACTGCATCAACATCCGCACGGGGCAGGAATGTCGGGATACTCAGCCCCCGGATGGA
AAGTCAAAAGACTGCATGCTCCAGATTGTTTGTCGAGATGGGAAAACAATTAGTCTTTGTGCAGAAAGCA
CAGATGATTGCTTGGCCTGGAAATTTACACTCCAAGATTCTAGGACAAACACAGCGTATGTGGGCTCTGC
AGTCATGACCGATGAGACATCCGTGGTTTCCTCACCTCCACCATACACGGCCTATGCTGCACCGGCCCCT
GAGCAGGCTTATGGCTATGGGCCATACGGTGGTGCGTACCCGCCAGGAACTCAAGTTGTCTACGCTGCGA
ATGGGCAGGCGTATGCCGTGCCCTACCAGTACCCATATGCAGGACTTTATGGACAGCAGCCTGCTAACCA
AGTCATCATTCGAGAGCGCTATCGAGACAACGACAGCGACCTGGCACTGGGCATGCTGGCAGGAGCAGCC
ACGGGCATGGCCTTAGGGTCTCTATTTTGGGTCTTC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC204118 protein sequence
Red=Cloning site Green=Tags(s)

MAFVKSGWLLRQSTILKRWKKNWFDLWSDGHLIYYDDQTRQNIEDKVHMPMDCINIRTGQECRDTQPPDG
KSKDCMLQIVCRDGKTISLCAESTDDCLAWKFTLQDSRTNTAYVGSAVMTDETSVVSSPPPYTAYAAPAP
EQAYGYGPYGGAYPPGTQVVYAANGQAYAVPYQYPYAGLYGQQPANQVIIRERYRDNDSDLALGMLAGAA
TGMALGSLFWVF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_017958
ORF Size 666 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_017958.2
RefSeq Size 4323 bp
RefSeq ORF 666 bp
Locus ID 55041
UniProt ID Q96CS7
Cytogenetics 2q21.1
Domains PH
MW 24.7 kDa
Summary Involved in retrograde transport of recycling endosomes.UniProtKB/Swiss-Prot Function
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"NM_017958" in other vectors (4)
SKU Description Size Price
RC204118L3 Lenti ORF clone of Human pleckstrin homology domain containing, family B (evectins) member 2 (PLEKHB2), transcript variant 2, Myc-DDK-tagged 10 ug
$600.00
RC204118L4 Lenti ORF clone of Human pleckstrin homology domain containing, family B (evectins) member 2 (PLEKHB2), transcript variant 2, mGFP tagged 10 ug
$600.00
RG204118 PLEKHB2 (tGFP-tagged) - Human pleckstrin homology domain containing, family B (evectins) member 2 (PLEKHB2), transcript variant 2 10 ug
$489.00 $500.00
SC319921 PLEKHB2 (untagged)-Human pleckstrin homology domain containing, family B (evectins) member 2 (PLEKHB2), transcript variant 2 10 ug
$300.00

Plasmid Map for RC204118

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC204118
Circular map for RC204118

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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