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DMAC2L (NM_001003805) Human Tagged ORF Clone

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SKU
RC204106
ATP5S (Myc-DDK-tagged)-Human ATP synthase, H+ transporting, mitochondrial Fo complex, subunit s (factor B) (ATP5S), nuclear gene encoding mitochondrial protein, transcript variant 2
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $150.00
In Stock*
Bulk Requests & Clone Modifications

Frequently Bought Together (6)

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Other products for "DMAC2L or NM_001003805"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol DMAC2L
Synonyms ATP5S; ATPW; FB; HSU79253
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC204106 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTGCTGTGCGGTCTCTGAGCAGCGACTCACCTGTGCAGATCAAATGATGCTGTTTGGAAAAATTTCCC
AGCAGTTGTGTGGCGTAAAGAAACTCCCATGGTCATGTGACTCCAGATACTTCTGGGGCTGGTTGAATGC
AGTGTTTAATAAGGTGGATTATGATCGCATCAGGGATGTTGGCCCTGATAGGGCGGCATCCGAGTGGTTG
CTGCGCTGTGGGGCCATGGTGCGCTACCATGGCCAGGAGAGGTGGCAGAAGGACTACAACCACCTTCCAA
CAGGCCCTCTGGACAAATACAAGATTCAGGCGATCGACGCCACCGACTCTTGTATCATGAGCATTGGATT
TGATCACATGGAAACCTCAAATATTTGTTGT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC204106 protein sequence
Red=Cloning site Green=Tags(s)

MCCAVSEQRLTCADQMMLFGKISQQLCGVKKLPWSCDSRYFWGWLNAVFNKVDYDRIRDVGPDRAASEWL
LRCGAMVRYHGQERWQKDYNHLPTGPLDKYKIQAIDATDSCIMSIGFDHMETSNICC

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001003805
ORF Size 381 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001003805.2, NP_001003805.1
RefSeq Size 1687 bp
RefSeq ORF 339 bp
Locus ID 27109
UniProt ID Q99766
Cytogenetics 14q21.3
MW 14.7 kDa
Summary This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. This gene encodes the subunit s, also known as factor B, of the proton channel. This subunit is necessary for the energy transduction activity of the ATP synthase complexes. Alternatively spliced transcript variants encoding different isoforms have been identified. provided by RefSeq, Jul 2008
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"NM_001003805" in other vectors (5)
SKU Description Size Price
RC204106L3 Lenti ORF clone of Human ATP synthase, H+ transporting, mitochondrial Fo complex, subunit s (factor B) (ATP5S), nuclear gene encoding mitochondrial protein, transcript variant 2, Myc-DDK-tagged 10 ug
$450.00
RC204106L4 Lenti ORF clone of Human ATP synthase, H+ transporting, mitochondrial Fo complex, subunit s (factor B) (ATP5S), nuclear gene encoding mitochondrial protein, transcript variant 2, mGFP tagged 10 ug
$450.00
RG204106 ATP5S (tGFP-tagged) - Human ATP synthase, H+ transporting, mitochondrial Fo complex, subunit s (factor B) (ATP5S), nuclear gene encoding mitochondrial protein, transcript variant 2 10 ug
$489.00
SC300556 ATP5S (untagged)-Human ATP synthase, H+ transporting, mitochondrial Fo complex, subunit s (factor B) (ATP5S), nuclear gene encoding mitochondrial protein, transcript variant 2 10 ug
$165.00
SC320790 ATP5S (untagged)-Human ATP synthase, H+ transporting, mitochondrial Fo complex, subunit s (factor B) (ATP5S), nuclear gene encoding mitochondrial protein, transcript variant 2 10 ug
$150.00

Plasmid Map for RC204106

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC204106
Circular map for RC204106

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