NOSIP (NM_015953) Human Tagged ORF Clone

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SKU
RC204024
NOSIP (Myc-DDK-tagged)-Human nitric oxide synthase interacting protein (NOSIP)
  $300.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol NOSIP
Synonyms CGI-25
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC204024 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACGCGGCATGGCAAGAACTGCACCGCAGGGGCCGTCTACACCTACCACGAGAAGAAGAAGGACACAG
CGGCCTCGGGCTATGGGACCCAGAACATTCGACTGAGCCGGGATGCCGTGAAGGACTTCGACTGCTGTTG
TCTCTCCCTGCAGCCTTGCCACGATCCTGTTGTCACCCCAGATGGCTACCTGTATGAGCGTGAGGCCATC
CTGGAGTACATTCTGCACCAGAAGAAGGAGATTGCCCGGCAGATGAAGGCCTACGAGAAGCAGCGGGGCA
CCCGGCGCGAGGAGCAGAAGGAGCTTCAGCGGGCGGCCTCGCAGGACCATGTGCGGGGCTTCCTGGAGAA
GGAGTCGGCTATCGTGAGCCGGCCCCTCAACCCTTTCACAGCCAAGGCCCTCTCGGGCACCAGCCCAGAT
GATGTCCAACCTGGGCCCAGTGTGGGTCCTCCAAGTAAGGACAAGGACAAAGTGCTGCCCAGCTTCTGGA
TCCCGTCGCTGACGCCCGAAGCCAAGGCCACCAAGCTGGAGAAGCCGTCCCGCACGGTGACCTGCCCCAT
GTCAGGGAAGCCCCTGCGCATGTCGGACCTGACGCCCGTGCACTTCACACCGCTAGACAGCTCCGTGGAC
CGCGTGGGGCTCATCACCCGCAGCGAGCGCTACGTGTGTGCCGTGACCCGCGACAGCCTGAGCAACGCCA
CCCCCTGCGCTGTGCTGCGGCCCTCTGGGGCTGTGGTCACCCTCGAATGCGTGGAGAAGCTGATTCGGAA
GGACATGGTGGACCCTGTGACTGGAGACAAACTCACAGACCGCGACATCATCGTGCTGCAGCGGGGCGGT
ACCGGCTTCGCGGGCTCCGGAGTGAAGCTGCAAGCGGAGAAATCACGGCCGGTGATGCAGGCC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC204024 protein sequence
Red=Cloning site Green=Tags(s)

MTRHGKNCTAGAVYTYHEKKKDTAASGYGTQNIRLSRDAVKDFDCCCLSLQPCHDPVVTPDGYLYEREAI
LEYILHQKKEIARQMKAYEKQRGTRREEQKELQRAASQDHVRGFLEKESAIVSRPLNPFTAKALSGTSPD
DVQPGPSVGPPSKDKDKVLPSFWIPSLTPEAKATKLEKPSRTVTCPMSGKPLRMSDLTPVHFTPLDSSVD
RVGLITRSERYVCAVTRDSLSNATPCAVLRPSGAVVTLECVEKLIRKDMVDPVTGDKLTDRDIIVLQRGG
TGFAGSGVKLQAEKSRPVMQA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_015953
ORF Size 903 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_015953.4
RefSeq Size 1367 bp
RefSeq ORF 906 bp
Locus ID 51070
UniProt ID Q9Y314
Cytogenetics 19q13.33
MW 33.2 kDa
Summary The protein encoded by this gene may modulate the activity and localization of nitric oxide synthase (endothelial and neuronal) and thus nitric oxide production. Alternative splicing results in multiple transcript variants that encode the same protein. provided by RefSeq, Aug 2012
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Documents
Resources
"NM_015953" in other vectors (5)
SKU Description Size Price
RC204024L3 Lenti ORF clone of Human nitric oxide synthase interacting protein (NOSIP), Myc-DDK-tagged 10 ug
$600.00
RC204024L4 Lenti ORF clone of Human nitric oxide synthase interacting protein (NOSIP), mGFP tagged 10 ug
$600.00
RG204024 NOSIP (tGFP-tagged) - Human nitric oxide synthase interacting protein (NOSIP) 10 ug
$489.00 $500.00
SC108090 NOSIP (untagged)-Human nitric oxide synthase interacting protein (NOSIP) 10 ug
$300.00
SC320872 NOSIP (untagged)-Human nitric oxide synthase interacting protein (NOSIP) 10 ug
$300.00

Plasmid Map for RC204024

Download sequence for

Cloning scheme for RC204024

Circular map for RC204024

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