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G3BP2 (NM_203504) Human Tagged ORF Clone

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SKU
RC203977
G3BP2 (Myc-DDK-tagged)-Human GTPase activating protein (SH3 domain) binding protein 2 (G3BP2), transcript variant 3
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $457.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "G3BP2 or NM_203504"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol G3BP2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC203977 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTTATGGAGAAGCCCAGTCCGCTGCTTGTAGGGCGGGAGTTTGTGAGGCAATATTATACTTTGCTGA
ATAAAGCTCCGGAATATTTACACAGGTTTTATGGCAGGAATTCTTCCTATGTTCATGGTGGAGTAGATGC
TAGTGGAAAGCCCCAGGAAGCTGTTTATGGCCAAAATGATATACACCACAAAGTATTATCTCTGAACTTC
AGTGAATGTCATACTAAAATTCGTCATGTGGATGCTCATGCAACCTTGAGTGATGGAGTAGTTGTCCAGG
TCATGGGTTTGCTGTCTAACAGTGGACAACCAGAAAGAAAGTTTATGCAAACCTTTGTTCTGGCTCCTGA
AGGATCTGTTCCAAATAAATTTTATGTTCACAATGATATGTTTCGTTATGAAGATGAAGTGTTTGGTGAT
TCTGAGCCTGAACTTGATGAAGAATCAGAAGATGAAGTAGAAGAGGAACAAGAAGAAAGACAACCATCTC
CTGAACCTGTGCAAGAAAATGCTAACAGTGGTTACTATGAAGCTCACCCTGTGACTAATGGCATAGAGGA
GCCTTTGGAAGAATCCTCTCATGAACCTGAACCTGAGCCAGAATCTGAAACAAAGACTGAAGAGCTGAAA
CCACAAGTGGAGGAGAAGAACTTAGAAGAACTAGAGGAGAAATCTACTACTCCTCCTCCGGCAGAACCTG
TTTCTCTGCCACAAGAACCACCAAAGCCAAGAGTCGAAGCTAAACCAGAAGTTCAATCTCAGCCACCTCG
TGTGCGTGAACAACGACCTAGAGAACGACCTGGTTTTCCTCCTAGAGGACCAAGACCAGGCAGAGGAGAT
ATGGAACAGAATGACTCTGACAACCGTAGAATAATTCGCTATCCAGATAGTCATCAACTTTTTGTTGGTA
ACTTGCCACATGATATTGATGAAAATGAGCTAAAGGAATTCTTCATGAGTTTTGGAAACGTTGTGGAACT
TCGCATCAATACCAAGGGTGTTGGGGGAAAGCTTCCAAATTTTGGTTTTGTGGTTTTTGATGACTCTGAA
CCAGTTCAGAGAATCTTAATTGCAAAACCGATTATGTTTCGAGGGGAAGTACGTTTAAATGTGGAAGAGA
AAAAAACAAGAGCTGCAAGAGAGCGAGAAACCAGAGGTGGTGGTGATGATCGCAGGGATATTAGGCGCAA
TGATCGAGGTCCCGGTGGTCCACGTGGAATTGTGGGTGGTGGAATGATGCGTGATCGTGATGGAAGAGGA
CCTCCTCCAAGGGGTGGCATGGCACAGAAACTTGGCTCTGGAAGAGGAACCGGGCAAATGGAGGGCCGCT
TCACAGGACAGCGTCGC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC203977 protein sequence
Red=Cloning site Green=Tags(s)

MVMEKPSPLLVGREFVRQYYTLLNKAPEYLHRFYGRNSSYVHGGVDASGKPQEAVYGQNDIHHKVLSLNF
SECHTKIRHVDAHATLSDGVVVQVMGLLSNSGQPERKFMQTFVLAPEGSVPNKFYVHNDMFRYEDEVFGD
SEPELDEESEDEVEEEQEERQPSPEPVQENANSGYYEAHPVTNGIEEPLEESSHEPEPEPESETKTEELK
PQVEEKNLEELEEKSTTPPPAEPVSLPQEPPKPRVEAKPEVQSQPPRVREQRPRERPGFPPRGPRPGRGD
MEQNDSDNRRIIRYPDSHQLFVGNLPHDIDENELKEFFMSFGNVVELRINTKGVGGKLPNFGFVVFDDSE
PVQRILIAKPIMFRGEVRLNVEEKKTRAARERETRGGGDDRRDIRRNDRGPGGPRGIVGGGMMRDRDGRG
PPPRGGMAQKLGSGRGTGQMEGRFTGQRR

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_203504
ORF Size 1347 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_203504.2, NP_987100.1
RefSeq Size 4311 bp
RefSeq ORF 1350 bp
Locus ID 9908
UniProt ID Q9UN86
Cytogenetics 4q21.1
Protein Families Druggable Genome
MW 50.8 kDa
Summary Scaffold protein that plays an essential role in cytoplasmic stress granule formation which acts as a platform for antiviral signaling.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_203504" in other vectors (4)
SKU Description Size Price
RC203977L3 Lenti ORF clone of Human GTPase activating protein (SH3 domain) binding protein 2 (G3BP2), transcript variant 3, Myc-DDK-tagged 10 ug
$757.00
RC203977L4 Lenti ORF clone of Human GTPase activating protein (SH3 domain) binding protein 2 (G3BP2), transcript variant 3, mGFP tagged 10 ug
$757.00
RG203977 G3BP2 (tGFP-tagged) - Human GTPase activating protein (SH3 domain) binding protein 2 (G3BP2), transcript variant 3 10 ug
$489.00 $657.00
SC308217 G3BP2 (untagged)-Human GTPase activating protein (SH3 domain) binding protein 2 (G3BP2), transcript variant 3 10 ug
$503.00

Plasmid Map for RC203977

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC203977
Circular map for RC203977

file-alt Citations

A GTPase-activating protein binding protein (G3BP1) / antiviral protein relay conveys arteriosclerotic Wnt signals in aortic smooth muscle cells
Ramachandran, Stabley, Cheng et al
J. Biol. Chem. (2018)
AI Summary: In this study, OriGene’s TrueORF Gold plasmids were used for transfection experiments to investigate the molecular mechanisms by which G3BP1 methylation and its interactions with RIG-I and MAVS contribute to Wnt signaling and arteriosclerotic processes in aortic smooth muscle cells.
Snippet: The remaining plasmids were purchased either from Origene (Tru eORF Gold) or from Addgene as indicated. For plasmid transfection, HEK293T cells (ATCC;CRL -3216) or MOVAS mouse aortic smooth muscle cells by guest on April 8, 2018 http://www.jbc.org/ Downloaded from 13 (ATCC;CRL -2797)(29,30) were seeded the day before at 65,000 cells/well in 12 well culture plates and transient transfections and ...
Showing 1-1 of 1 papers.
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