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GTF3C5 (NM_012087) Human Tagged ORF Clone

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SKU
RC203953
GTF3C5 (Myc-DDK-tagged)-Human general transcription factor IIIC, polypeptide 5, 63kDa (GTF3C5), transcript variant 2
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $483.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol GTF3C5
Synonyms TFiiiC2-63; TFIIIC63; TFIIICepsilon
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC203953 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGCGGAGGCGGCCGATTTGGGGCTGGGGGCCGCCGTCCCCGTGGAGCTGAGGCGGGAGCGACGCA
TGGTGTGCGTGGAGTACCCGGGAGTGGTGCGTGATGTGGCTAAGATGCTGCCGACTCTGGGCGGCGAGGA
AGGCGTCTCCCGGATCTACGCAGACCCCACCAAGAGGCTGGAGCTGTACTTCCGGCCCAAGGACCCATAC
TGCCACCCAGTGTGCGCCAACCGCTTCAGTACCAGCAGCCTGCTGCTCCGCATCAGGAAGAGAACGAGGC
GGCAGAAAGGGGTGCTGGGCACTGAGGCCCACTCCGAGGTCACATTTGACATGGAGATCCTTGGCATCAT
CTCCACCATTTACAAATTTCAGGGGATGTCTGACTTCCAGTACTTGGCTGTGCATACGGAAGCAGGCGGC
AAGCATACGTCAATGTATGACAAGGTGCTCATGCTCCGGCCCGAGAAGGAGGCCTTTTTCCACCAGGAGC
TGCCGCTCTACATCCCCCCACCCATCTTCTCCCGGCTGGACGCCCCGGTGGACTACTTCTACCGACCAGA
GACCCAGCACCGGGAAGGCTACAACAATCCCCCCATCTCAGGTGAGAATCTGATTGGCCTGAGCAGAGCC
CGGCGCCCCCACAATGCCATCTTTGTCAACTTTGAGGATGAGGAGGTGCCCAAGCAGCCACTGGAGGCTG
CAGCCCAGACGTGGAGGAGAGTCTGCACTAACCCCGTGGACCGGAAGGTGGAGGAGGAGCTGAGGAAGCT
GTTTGACATCCGTCCCATCTGGTCCCGAAATGCTGTCAAGGCCAACATCAGCGTCCACCCAGACAAGCTC
AAGGTCTTGCTTCCCTTCATAGCCTATTACATGATAACAGGCCCCTGGCGCAGCCTATGGATTCGATTTG
GGTATGACCCCCGAAAAAACCCAGATGCCAAGATTTATCAAGTCCTCGATTTCCGAATCCGTTGTGGAAT
GAAACACGGTTACGCCCCCAGTGACTTGCCGGTCAAAGCAAAGCGCAGCACCTACAACTACAGCCTCCCC
ATCACCGTCAAGAAGACATCCAGCCAGCTTGTCACCATGCATGACCTGAAGCAGGGCCTGGGCCCGTCGG
GGACGAGTGGTGCTCGGAAACCAGCTTCCAGCAAGTACAAGCTCAAGGACTCTGTCTACATCTTCCGGGA
AGGGGCCTTGCCACCCTATCGGCAGATGTTCTACCAGTTATGCGACTTGAATGTGGAAGAGTTGCAGAAG
ATCATTCACCGCAATGACGGGGCAGAGAATTCCTGCACAGAACGGGATGGGTGGTGCCTCCCCAAGACCA
GCGACGAGCTCAGGGACACCATGTCCCTCATGATCCGGCAGACCATCCGCTCCAAGAGGCCTGCTCTCTT
TTCCAGCTCAGCCAAGGCTGATGGCGGAAAAGAGCAGCTGACGTACGAGTCTGGGGAAGACGAGGAGGAT
GAGGAGGAGGAGGAAGAGGAGGAGGAGGACTTCAAGCCATCCGACGGCAGTGAAAACGAAATGGAGACAG
AGATTCTGGACTACGTG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC203953 protein sequence
Red=Cloning site Green=Tags(s)

MAAEAADLGLGAAVPVELRRERRMVCVEYPGVVRDVAKMLPTLGGEEGVSRIYADPTKRLELYFRPKDPY
CHPVCANRFSTSSLLLRIRKRTRRQKGVLGTEAHSEVTFDMEILGIISTIYKFQGMSDFQYLAVHTEAGG
KHTSMYDKVLMLRPEKEAFFHQELPLYIPPPIFSRLDAPVDYFYRPETQHREGYNNPPISGENLIGLSRA
RRPHNAIFVNFEDEEVPKQPLEAAAQTWRRVCTNPVDRKVEEELRKLFDIRPIWSRNAVKANISVHPDKL
KVLLPFIAYYMITGPWRSLWIRFGYDPRKNPDAKIYQVLDFRIRCGMKHGYAPSDLPVKAKRSTYNYSLP
ITVKKTSSQLVTMHDLKQGLGPSGTSGARKPASSKYKLKDSVYIFREGALPPYRQMFYQLCDLNVEELQK
IIHRNDGAENSCTERDGWCLPKTSDELRDTMSLMIRQTIRSKRPALFSSSAKADGGKEQLTYESGEDEED
EEEEEEEEEDFKPSDGSENEMETEILDYV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_012087
ORF Size 1557 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_012087.4
RefSeq Size 2436 bp
RefSeq ORF 1560 bp
Locus ID 9328
UniProt ID Q9Y5Q8
Cytogenetics 9q34.13
Protein Families Transcription Factors
MW 59.6 kDa
Summary Involved in RNA polymerase III-mediated transcription. Integral, tightly associated component of the DNA-binding TFIIIC2 subcomplex that directly binds tRNA and virus-associated RNA promoters.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_012087" in other vectors (5)
SKU Description Size Price
RC203953L3 Lenti ORF clone of Human general transcription factor IIIC, polypeptide 5, 63kDa (GTF3C5), transcript variant 2, Myc-DDK-tagged 10 ug
$783.00
RC203953L4 Lenti ORF clone of Human general transcription factor IIIC, polypeptide 5, 63kDa (GTF3C5), transcript variant 2, mGFP tagged 10 ug
$783.00
RG203953 GTF3C5 (tGFP-tagged) - Human general transcription factor IIIC, polypeptide 5, 63kDa (GTF3C5), transcript variant 2 10 ug
$683.00
SC115538 GTF3C5 (untagged)-Human general transcription factor IIIC, polypeptide 5, 63kDa (GTF3C5), transcript variant 2 10 ug
$484.00
SC322243 GTF3C5 (untagged)-Human general transcription factor IIIC, polypeptide 5, 63kDa (GTF3C5), transcript variant 2 10 ug
$484.00

Plasmid Map for RC203953

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC203953
Circular map for RC203953

file-alt Citations

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