RGR (NM_002921) Human Tagged ORF Clone

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SKU
RC203778
RGR (Myc-DDK-tagged)-Human retinal G protein coupled receptor (RGR), transcript variant 1
  $300.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol RGR
Synonyms RP44
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC203778 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAGAGACCAGTGCCTTGCCCACCGGCTTCGGGGAGCTCGAGGTGCTGGCTGTGGGGATGGTGCTAC
TGGTGGAAGCTCTCTCCGGTCTCAGCCTCAATACCCTGACCATCTTCTCTTTCTGCAAGACCCCGGAGCT
GCGGACTCCCTGCCACCTACTGGTGCTGAGCTTGGCTCTTGCGGACAGTGGGATCAGCCTGAATGCCCTC
GTTGCAGCCACATCCAGCCTTCTCCGTGTCTCCCACAGGCGCTGGCCCTACGGCTCGGACGGCTGCCAGG
CTCACGGCTTCCAGGGCTTTGTGACAGCGTTGGCCAGCATCTGCAGCAGTGCAGCCATCGCATGGGGGCG
TTATCACCACTACTGCACCCGTAGCCAGCTGGCCTGGAACTCAGCCGTCTCTCTGGTGCTCTTCGTGTGG
CTGTCTTCTGCCTTCTGGGCAGCTCTGCCCCTTCTGGGTTGGGGTCACTATGACTATGAGCCACTGGGGA
CATGCTGCACCCTGGACTACTCCAAGGGGGACAGAAACTTCACCAGCTTCCTCTTCACCATGTCCTTCTT
CAACTTCGCCATGCCCCTCTTCATCACGATCACTTCCTACAGTCTCATGGAGCAGAAACTGGGGAAGAGT
GGCCATCTCCAGGTAAACACCACTCTGCCAGCAAGGACGCTGCTGCTCGGCTGGGGCCCCTATGCCATCC
TGTATCTATACGCAGTCATCGCAGACGTGACTTCCATCTCCCCCAAACTGCAGATGGTGCCCGCCCTCAT
TGCCAAAATGGTGCCCACGATCAATGCCATCAACTATGCCCTGGGCAATGAGATGGTCTGCAGGGGAATC
TGGCAGTGCCTCTCACCGCAGAAGAGGGAGAAGGACCGAACCAAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC203778 protein sequence
Red=Cloning site Green=Tags(s)

MAETSALPTGFGELEVLAVGMVLLVEALSGLSLNTLTIFSFCKTPELRTPCHLLVLSLALADSGISLNAL
VAATSSLLRVSHRRWPYGSDGCQAHGFQGFVTALASICSSAAIAWGRYHHYCTRSQLAWNSAVSLVLFVW
LSSAFWAALPLLGWGHYDYEPLGTCCTLDYSKGDRNFTSFLFTMSFFNFAMPLFITITSYSLMEQKLGKS
GHLQVNTTLPARTLLLGWGPYAILYLYAVIADVTSISPKLQMVPALIAKMVPTINAINYALGNEMVCRGI
WQCLSPQKREKDRTK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_002921
ORF Size 885 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_002921.2
RefSeq Size 1475 bp
RefSeq ORF 888 bp
Locus ID 5995
UniProt ID P47804, A0A0S2Z498, P47804-2
Cytogenetics 10q23.1
Domains 7tm_1
Protein Families Druggable Genome, GPCR, Transmembrane
MW 32.4 kDa
Summary This gene encodes a putative retinal G-protein coupled receptor. The gene is a member of the opsin subfamily of the 7 transmembrane, G-protein coupled receptor 1 family. Like other opsins which bind retinaldehyde, it contains a conserved lysine residue in the seventh transmembrane domain. The protein acts as a photoisomerase to catalyze the conversion of all-trans-retinal to 11-cis-retinal. The reverse isomerization occurs with rhodopsin in retinal photoreceptor cells. The protein is exclusively expressed in tissue adjacent to retinal photoreceptor cells, the retinal pigment epithelium and Mueller cells. This gene may be associated with autosomal recessive and autosomal dominant retinitis pigmentosa (arRP and adRP, respectively). Alternative splicing results in multiple transcript variants encoding different isoforms. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_002921" in other vectors (7)
SKU Description Size Price
RC203778L1 Lenti ORF clone of Human retinal G protein coupled receptor (RGR), transcript variant 1, Myc-DDK-tagged 10 ug
$600.00
RC203778L2 Lenti ORF clone of Human retinal G protein coupled receptor (RGR), transcript variant 1, mGFP tagged 10 ug
$600.00
RC203778L3 Lenti ORF clone of Human retinal G protein coupled receptor (RGR), transcript variant 1, Myc-DDK-tagged 10 ug
$600.00
RC203778L4 Lenti ORF clone of Human retinal G protein coupled receptor (RGR), transcript variant 1, mGFP tagged 10 ug
$600.00
RG203778 RGR (tGFP-tagged) - Human retinal G protein coupled receptor (RGR), transcript variant 1 10 ug
$500.00
SC310563 RGR (untagged)-Human retinal G protein coupled receptor (RGR), transcript variant 1 10 ug
$300.00
SC322246 RGR (untagged)-Human retinal G protein coupled receptor (RGR), transcript variant 1 10 ug
$300.00

Plasmid Map for RC203778

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Cloning scheme for RC203778

Circular map for RC203778

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