OXCT1 (NM_000436) Human Tagged ORF Clone
SKU
RC203764
OXCT1 (Myc-DDK-tagged)-Human 3-oxoacid CoA transferase 1 (OXCT1), nuclear gene encoding mitochondrial protein
$289.00
$484.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | OXCT1 |
| Synonyms | OXCT; SCOT |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_000436 |
| ORF Size | 1560 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_000436.4 |
| RefSeq Size | 3572 bp |
| RefSeq ORF | 1563 bp |
| Locus ID | 5019 |
| UniProt ID | P55809, A0A024R040 |
| Cytogenetics | 5p13.1 |
| Domains | CoA_trans |
| Protein Pathways | Butanoate metabolism, leucine and isoleucine degradation, Synthesis and degradation of ketone bodies, Valine |
| MW | 56.2 kDa |
| Summary | This gene encodes a member of the 3-oxoacid CoA-transferase gene family. The encoded protein is a homodimeric mitochondrial matrix enzyme that plays a central role in extrahepatic ketone body catabolism by catalyzing the reversible transfer of coenzyme A from succinyl-CoA to acetoacetate. Mutations in this gene are associated with succinyl CoA:3-oxoacid CoA transferase deficiency. provided by RefSeq, Jul 2008 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_000436" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC203764L1 | Lenti ORF clone of Human 3-oxoacid CoA transferase 1 (OXCT1), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged | 10 ug |
$784.00
|
|
| RC203764L2 | Lenti ORF clone of Human 3-oxoacid CoA transferase 1 (OXCT1), nuclear gene encoding mitochondrial protein, mGFP tagged | 10 ug |
$784.00
|
|
| RC203764L3 | Lenti ORF clone of Human 3-oxoacid CoA transferase 1 (OXCT1), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged | 10 ug |
$784.00
|
|
| RC203764L4 | Lenti ORF clone of Human 3-oxoacid CoA transferase 1 (OXCT1), nuclear gene encoding mitochondrial protein, mGFP tagged | 10 ug |
$784.00
|
|
| RG203764 | OXCT1 (tGFP-tagged) - Human 3-oxoacid CoA transferase 1 (OXCT1), nuclear gene encoding mitochondrial protein | 10 ug |
$489.00
$684.00
|
|
| SC119890 | OXCT1 (untagged)-Human 3-oxoacid CoA transferase 1 (OXCT1), nuclear gene encoding mitochondrial protein | 10 ug |
$485.00
|
|
Citations
Identification of the regulatory elements and protein substrates of lysine acetoacetylation
Fu, Nguyen, Kumar et al
Elife (2026) 14 DOI: 10.7554/eLife.104123
Fu, Nguyen, Kumar et al
Elife (2026) 14 DOI: 10.7554/eLife.104123
AI Summary: OriGene products, specifically the Human AACS (Cat#: RC206247) and SCOT (Cat#: RC203764) transfected constructs, were used in this study to investigate the regulatory elements and protein substrates involved in lysine acetoacetylation.
Snippet: Transfected construct (Human) AACS OriGene Technologies Cat#: RC206247 Transfected construct (Human) SCOT OriGene Technologies Cat#: RC203764
Identification of the Regulatory Elements and Protein Substrates of Lysine Acetoacetylation
Fu, Nguyen, Kumar et al
bioRxiv (2024)
Fu, Nguyen, Kumar et al
bioRxiv (2024)
Snippet: HEK293T cells or HepG2 cells were cultured and transfected with pCMVβ-p300-myc (Addgene, Cat# 30489), flag-HDAC3 (Addgene, Cat# 13819), AACS (OriGene Technologies, Cat# RC206247), SCOT (OriGene Technologies, Cat# RC203764) or HMGCR (Addgene, Cat# 86085) by using Lipofectamine 3000 Transfection Reagent (ThermoFisher, Cat# L3000008)
Showing 1-2 of 2 papers.
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