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TRMT9B (NM_020844) Human Tagged ORF Clone

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SKU
RC203720
KIAA1456 (Myc-DDK-tagged)-Human KIAA1456 (KIAA1456), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol TRMT9B
Synonyms C8orf79; hTRM9L; KIAA1456; TRM9L
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC203720 representing NM_020844
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGATCATGAAGCCGCCCAGCTGGAGAAGCAGCATGTGCACAATGTGTACGAGAGCACAGCCCCTTACT
TCAGCGACCTGCAGAGCAAAGCCTGGCCTCGTGTCCGCCAGTTCCTGCAAGAGCAGAAGCCAGGCAGCCT
CATCGCTGACATAGGTTGTGGGACTGGAAAATATCTTAAAGTGAACAGCCAGGTACATACCGTGGGCTGT
GACTACTGTGGGCCACTGGTAGAGATTGCCCGGAATAGAGGATGTGAAGCCATGGTATGTGACAACCTTA
ATCTCCCCTTTAGGGATGAGGGCTTCGATGCCATCATCTCCATAGGAGTCATACATCATTTTTCTACAAA
ACAAAGAAGAATCAGAGCAATAAAAGAAATGGCCAGGGTCTTAGTTCCCGGAGGCCAACTGATGATTTAC
GTTTGGGCAATGGAACAAAAGAACCGTCGCTTTGAGAAGCAAGACGTGCTTGTTCCATGGAACAGGGCCC
TGTGTTCCCAGCTCTTCTCAGAGTCCAGCCAGTCTGGGAGGAAGAGGCAGTGTGGATACCCAGAAAGAGG
CCATCCCTACCATCCTCCTTGCTCTGAGTGTAGCTGTTCTGTTTGTTTTAAAGAGCAGGGTGGTTCAAAA
CGGTCCCACAGTGTGGGCTATGAACCTGCTATGGCAAGAACCTGTTTTGCAAATATTTCTAAGGAAGGCG
AGGAAGAATATGGATTTTACAGCACATTAGGAAAATCGTTTCGTTCCTGGTTTTTCTCCAGATCTTTGGA
TGAATCGACTCTGAGGAAGCAAATTGAAAGAGTAAGACCCTTGAAAAACACAGAAGTTTGGGCCAGTAGC
ACTGTAACAGTCCAGCCTTCCAGACACTCTAGTCTAGACTTTGATCACCAAGAGCCATTTTCAACAAAAG
AGCAAAGTTTAGATGAGGAAGTGTTTGTGGAATCTTCTTCTGGAAAACACTTGGAGTGGCTGAGAGCACC
AGGCACTCTGAAACATTTAAATGGAGACCATCAAGGGGAAATGAGGAGAAATGGAGGGGGAAATTTTCTG
GATAGCACTAATACTGGTGTGAATTGTGTGGATGCAGGCAACATAGAAGATGATAATCCTTCTGCTAGTA
AAATATTGAGAAGGATTTCTGCAGTCGATTCCACAGATTTCAACCCAGATGATACAATGTCTGTCGAAGA
TCCACAGACTGATGTTTTGGACTCCACAGCCTTTATGCGCTACTACCATGTGTTTCGAGAAGGGGAGCTC
TGCAGTCTGCTCAAGGAGAATGTGTCAGAGCTCCGTATCCTGAGTTCTGGGAATGATCATGGTAACTGGT
GTATCATTGCAGAGAAAAAGAGAGGTTGTGAT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC203720 representing NM_020844
Red=Cloning site Green=Tags(s)

MDHEAAQLEKQHVHNVYESTAPYFSDLQSKAWPRVRQFLQEQKPGSLIADIGCGTGKYLKVNSQVHTVGC
DYCGPLVEIARNRGCEAMVCDNLNLPFRDEGFDAIISIGVIHHFSTKQRRIRAIKEMARVLVPGGQLMIY
VWAMEQKNRRFEKQDVLVPWNRALCSQLFSESSQSGRKRQCGYPERGHPYHPPCSECSCSVCFKEQGGSK
RSHSVGYEPAMARTCFANISKEGEEEYGFYSTLGKSFRSWFFSRSLDESTLRKQIERVRPLKNTEVWASS
TVTVQPSRHSSLDFDHQEPFSTKEQSLDEEVFVESSSGKHLEWLRAPGTLKHLNGDHQGEMRRNGGGNFL
DSTNTGVNCVDAGNIEDDNPSASKILRRISAVDSTDFNPDDTMSVEDPQTDVLDSTAFMRYYHVFREGEL
CSLLKENVSELRILSSGNDHGNWCIIAEKKRGCD

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_020844
ORF Size 1362 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_020844.2, NP_065895.2
RefSeq Size 9603 bp
RefSeq ORF 1365 bp
Locus ID 57604
UniProt ID Q9P272
Cytogenetics 8p22
Protein Families Druggable Genome
MW 51.1 kDa
Summary May modifie wobble uridines in specific arginine and glutamic acid tRNAs. Acts as a tumor suppressor by promoting the expression of LIN9.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_020844" in other vectors (6)
SKU Description Size Price
RC203720L1 Lenti-ORF clone of KIAA1456 (Myc-DDK-tagged)-Human KIAA1456 (KIAA1456), transcript variant 1 10 ug
$757.00
RC203720L2 Lenti-ORF clone of KIAA1456 (mGFP-tagged)-Human KIAA1456 (KIAA1456), transcript variant 1 10 ug
$757.00
RC203720L3 Lenti-ORF clone of KIAA1456 (Myc-DDK-tagged)-Human KIAA1456 (KIAA1456), transcript variant 1 10 ug
$757.00
RC203720L4 Lenti-ORF clone of KIAA1456 (mGFP-tagged)-Human KIAA1456 (KIAA1456), transcript variant 1 10 ug
$757.00
RG203720 KIAA1456 (tGFP-tagged) - Human KIAA1456 (KIAA1456), transcript variant 1 10 ug
$489.00 $657.00
SC317744 KIAA1456 (untagged)-Human KIAA1456 (KIAA1456), transcript variant 1 10 ug
$503.00

Plasmid Map for RC203720

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC203720
Circular map for RC203720

file-alt Citations

CHIKV infection reprograms codon optimality to favor viral RNA translation by altering the tRNA epitranscriptome
Jungfleisch, Böttcher, Talló-Parra et al
Nat Commun (2022) 13 (1), 4725 DOI: 10.1038/s41467-022-31835-x
Cited by 59
AI Summary: In this study, the Myc-DDK-tagged KIAA1456-ORF plasmid from OriGene (Cat# RC203720) was used to overexpress KIAA1456 and investigate its role in CHIKV-induced reprogramming of host translation machinery.
Snippet: te coated with Poly-L-Lysine. The next day, cells were pretreated with OptiMEM (ThermoFisher) for 30 min and then transfected using lipofectamine (i) with the (Myc-DDK-tagged) KIAA1456-ORF plasmid (Origene, Cat#RC203720) for the KIAA1456 overexpression experiments or (ii) with the ON-TARGETplus Non-Targeting Pool siRNA (Cultek, Cat#RC77D-001810-10-05) or the ON-TARGETplus SMARTPool Human KIAA145
FigureFigureFigure
Showing 1-1 of 1 papers.
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