DTX2 (NM_020892) Human Tagged ORF Clone
SKU
RC203717
DTX2 (Myc-DDK-tagged)-Human deltex homolog 2 (Drosophila) (DTX2), transcript variant 1
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TrueORF®
TrueORF®
Expression-ready ORF plasmid with C-terminal tag(s)
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Product Data | |
Type | Human Tagged ORF Clone |
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Target Symbol | DTX2 |
Synonyms | RNF58 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
ORF Nucleotide Sequence
>RC203717 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCATGGCCCCAAGCCCTTCCCTGGTGCAGGTGTACACCAGCCCCGCGGCTGTGGCCGTGTGGGAAT GGCAGGACGGGCTGGGCACCTGGCACCCCTACAGTGCCACCGTCTGCAGCTTCATCGAGCAGCAGTTTGT CCAGCAGAAGGGCCAACGTTTTGGGCTTGGGAGCCTGGCCCACAGCATCCCCTTGGGCCAGGCAGACCCC TCGCTGGCCCCTTACATTATTGACCTCCCCAGCTGGACCCAGTTCCGCCAGGACACCGGCACCATGCGGG CTGTGCGGAGACACCTGTTCCCCCAGCACTCAGCCCCTGGCCGAGGTGTCGTCTGGGAGTGGCTGAGCGA CGATGGCTCCTGGACTGCCTATGAAGCCAGCGTCTGTGACTATCTGGAGCAGCAGGTGGCCAGGGGCAAC CAGCTCGTGGACTTGGCCCCCCTGGGGTACAACTACACTGTCAACTACACCACCCACACGCAGACCAACA AGACTTCCAGCTTTTGCCGCAGCGTGCGGCGCCAAGCAGGGCCGCCTTACCCGGTGACCACCATCATCGC TCCGCCGGGCCACACAGGCGTCGCCTGCTCTTGCCACCAGTGCCTCAGTGGCAGCAGAACTGGCCCCGTG TCAGGCCGCTACCGCCACTCCATGACCAACCTCCCTGCATACCCCGTCCCCCAGCACCCCCCACACAGGA CCGCTTCTGTGTTTGGGACCCACCAGGCCTTTGCACCGTACAACAAACCCTCACTCTCCGGGGCCCGGTC TGCGCCCAGGCTGAACACCACCAACGCCTGGGGCGCAGCTCCTCCTTCCCTGGGGAGCCAGCCCCTCTAC CGCTCCAGCCTCTCCCACCTGGGACCGCAGCACCTGCCCCCAGGATCCTCCACCTCCGGTGCAGTCAGTG CCTCCCTCCCCAGCGGTCCCTCAAGCAGCCCAGGGAGCGTCCCTGCCACTGTGCCCATGCAGATGCCAAA GCCCAGCAGAGTCCAGCAGGCGCTCGCAGGCATGACGAGTGTTCTGATGTCAGCCATTGGACTCCCTGTG TGTCTTAGCCGCGCACCCCAGCCCACCAGCCCTCCCGCCTCCCGTCTGGCTTCCAAAAGTCACGGCTCAG TTAAGAGATTGAGGAAAATGTCCGTGAAAGGAGCGACCCCGAAGCCAGAGCCAGAGCCAGAGCAGGTCAT AAAAAACTACACGGAAGAGCTGAAAGTGCCCCCAGATGAGGACTGCATCATCTGCATGGAGAAGCTGTCC GCAGCGTCTGGATACAGCGATGTGACTGACAGCAAGGCAATCGGGTCCCTAGCTGTGGGCCACCTCACCA AGTGCAGCCATGCCTTCCACCTGCTGTGCCTCCTGGCCATGTACTGCAACGGCAATAAGGATGGAAGTCT GCAGTGTCCCTCCTGCAAAACCATCTATGGAGAGAAGACGGGGACCCAGCCCCAGGGAAAGATGGAGGTA TTACGGTTCCAGATGTCGCTCCCCGGCCACGAGGACTGCGGGACCATCCTCATAGTTTACAGCATTCCCC ATGGTATCCAGGGCCCTGAGCACCCCAATCCCGGAAAGCCGTTCACTGCCAGAGGGTTTCCCCGCCAGTG CTACCTTCCAGACAACGCCCAGGGCCGCAAGGTCCTAGAGCTCCTGAAGGTGGCCTGGAAGAGGCGGCTC ATCTTCACAGTGGGCACGTCCAGCACCACGGGTGAGACGGACACCGTGGTATGGAACGAGATCCACCACA AGACAGAGATGGACCGCAACATTACGGGCCACGGCTATCCCGACCCCAACTACCTGCAGAACGTGCTGGC TGAGCTGGCTGCCCAGGGGGTGACCGAGGACTGCCTGGAGCAGCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC203717 protein sequence
Red=Cloning site Green=Tags(s) MAMAPSPSLVQVYTSPAAVAVWEWQDGLGTWHPYSATVCSFIEQQFVQQKGQRFGLGSLAHSIPLGQADP SLAPYIIDLPSWTQFRQDTGTMRAVRRHLFPQHSAPGRGVVWEWLSDDGSWTAYEASVCDYLEQQVARGN QLVDLAPLGYNYTVNYTTHTQTNKTSSFCRSVRRQAGPPYPVTTIIAPPGHTGVACSCHQCLSGSRTGPV SGRYRHSMTNLPAYPVPQHPPHRTASVFGTHQAFAPYNKPSLSGARSAPRLNTTNAWGAAPPSLGSQPLY RSSLSHLGPQHLPPGSSTSGAVSASLPSGPSSSPGSVPATVPMQMPKPSRVQQALAGMTSVLMSAIGLPV CLSRAPQPTSPPASRLASKSHGSVKRLRKMSVKGATPKPEPEPEQVIKNYTEELKVPPDEDCIICMEKLS AASGYSDVTDSKAIGSLAVGHLTKCSHAFHLLCLLAMYCNGNKDGSLQCPSCKTIYGEKTGTQPQGKMEV LRFQMSLPGHEDCGTILIVYSIPHGIQGPEHPNPGKPFTARGFPRQCYLPDNAQGRKVLELLKVAWKRRL IFTVGTSSTTGETDTVVWNEIHHKTEMDRNITGHGYPDPNYLQNVLAELAAQGVTEDCLEQQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
Chromatograms
![]() Sequencher program is needed, download here |
Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_020892 |
ORF Size | 1866 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Shipping | Ambient |
Reference Data | |
RefSeq | NM_020892.1, NP_065943.1 |
RefSeq Size | 2835 bp |
RefSeq ORF | 1869 bp |
Locus ID | 113878 |
UniProt ID | Q86UW9 |
Cytogenetics | 7q11.23 |
Domains | RING, WWE |
Protein Families | Druggable Genome |
Protein Pathways | Notch signaling pathway |
MW | 67.2 kDa |
Summary | DTX2 functions as an E3 ubiquitin ligase (Takeyama et al., 2003 [PubMed 12670957]).[supplied by OMIM, Nov 2009] |
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Product Manuals |
FAQs |
SDS |
SKU | Description | Size | Price | |
---|---|---|---|---|
RC203717L3 | Lenti ORF clone of Human deltex homolog 2 (Drosophila) (DTX2), transcript variant 1, Myc-DDK-tagged | 10 ug |
$879.00
|
|
RC203717L4 | Lenti ORF clone of Human deltex homolog 2 (Drosophila) (DTX2), transcript variant 1, mGFP tagged | 10 ug |
$879.00
|
|
RG203717 | DTX2 (tGFP-tagged) - Human deltex homolog 2 (Drosophila) (DTX2), transcript variant 1 | 10 ug |
$489.00
MSRP
$779.00
MSRP
$779.00
|
|
SC321670 | DTX2 (untagged)-Human deltex homolog 2 (Drosophila) (DTX2), transcript variant 1 | 10 ug |
$580.00
|
|
Citations
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.