CCDC90A (MCUR1) (NM_001031713) Human Tagged ORF Clone

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SKU
RC203684
CCDC90A (Myc-DDK-tagged)-Human coiled-coil domain containing 90A (CCDC90A)
  $457.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol CCDC90A
Synonyms C6orf79; CCDC90A; FMP32
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC203684 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACTGCGGCTCGGTCGGCGGCCAGAGGACCCAGCGCCTGCCCGGCCGCCAGCGGCTTCTCTTCTTGC
CCGTGGGCCTCAGCGGAAGACCGGGCGGCAGCGAAACCTCAGCACGCCGCTGCCTCTCTGCGCTCTCCGA
CGGTCTGGGGGCGCTGCGCCCTCGCGCCCCGGCGGCCCGCGGCGGCGTGTCACGTGCCTCACCGCTGCTC
CTCCTCCTGCTAGTGCCCTCCCCGCGCTTGGCCGCCGCAGCCCCGCGCCGGCAGCTCGGGGACTGGGAGC
GCTCGCGCCTCGGGTATGCCGCACCCCCGGCCGGGCGCAGCAGCGCGTGGAGGTGCTCACCGGGCGTCGC
CGCTGCCGCCGGCGCCCTTCCCCAGTACCACGGCCCGGCGCCTGCACTCGTTTCCTGCAGGAGAGAGCTA
AGCTTGTCTGCTGGATCCCTGCAGCTGGAGCGCAAAAGGAGAGATTTCACCTCTTCTGGGAGCAGGAAAC
TCTACTTCGACACTCATGCCTTAGTGTGCTTACTGGAAGACAATGGGTTTGCTACTCAACAAGCAGAAAT
CATTGTGTCTGCATTGGTCAAGATCCTGGAGGCCAACATGGACATCGTCTACAAAGATATGGTCACCAAG
ATGCAGCAGGAAATCACTTTTCAGCAAGTAATGTCTCAGATTGCGAATGTGAAAAAGGATATGATTATTT
TGGAGAAGAGTGAATTTTCAGCCCTCAGAGCAGAAAATGAGAAAATAAAACTCGAACTACATCAGTTAAA
ACAACAAGTAATGGATGAAGTGATCAAAGTCCGAACAGATACCAAATTAGACTTCAACCTAGAAAAGAGC
AGAGTAAAAGAATTGTATTCATTGAACGAAAAGAAGCTGCTGGAATTGAGAACAGAAATAGTGGCATTGC
ATGCCCAGCAAGATCGGGCCCTTACCCAGACAGACAGGAAGATCGAAACTGAGGTTGCTGGCCTCAAAAC
CATGCTTGAGTCACACAAGCTTGATAATATTAAATATTTAGCAGGGTCTATATTTACGTGCCTAACAGTA
GCTCTGGGATTTTATCGCCTGTGGATC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC203684 protein sequence
Red=Cloning site Green=Tags(s)

MDCGSVGGQRTQRLPGRQRLLFLPVGLSGRPGGSETSARRCLSALSDGLGALRPRAPAARGGVSRASPLL
LLLLVPSPRLAAAAPRRQLGDWERSRLGYAAPPAGRSSAWRCSPGVAAAAGALPQYHGPAPALVSCRREL
SLSAGSLQLERKRRDFTSSGSRKLYFDTHALVCLLEDNGFATQQAEIIVSALVKILEANMDIVYKDMVTK
MQQEITFQQVMSQIANVKKDMIILEKSEFSALRAENEKIKLELHQLKQQVMDEVIKVRTDTKLDFNLEKS
RVKELYSLNEKKLLELRTEIVALHAQQDRALTQTDRKIETEVAGLKTMLESHKLDNIKYLAGSIFTCLTV
ALGFYRLWI

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001031713
ORF Size 1077 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001031713.3, NP_001026883.1
RefSeq Size 5472 bp
RefSeq ORF 1080 bp
Locus ID 63933
UniProt ID Q96AQ8
Cytogenetics 6p23
Protein Families Transmembrane
MW 39.7 kDa
Summary Key regulator of mitochondrial calcium uniporter (MCU) required for calcium entry into mitochondrion (PubMed:23178883, PubMed:26445506, PubMed:27184846, PubMed:26976564). Plays a direct role in uniporter-mediated calcium uptake via a direct interaction with MCU (PubMed:23178883). Probably involved in the assembly of the membrane components of the uniporter complex (uniplex) (PubMed:27184846).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001031713" in other vectors (6)
SKU Description Size Price
RC203684L1 Lenti ORF clone of Human coiled-coil domain containing 90A (CCDC90A), Myc-DDK-tagged 10 ug
$757.00
RC203684L2 Lenti ORF clone of Human coiled-coil domain containing 90A (CCDC90A), mGFP tagged 10 ug
$757.00
RC203684L3 Lenti ORF clone of Human coiled-coil domain containing 90A (CCDC90A), Myc-DDK-tagged 10 ug
$757.00
RC203684L4 Lenti ORF clone of Human coiled-coil domain containing 90A (CCDC90A), mGFP tagged 10 ug
$757.00
RG203684 CCDC90A (tGFP-tagged) - Human coiled-coil domain containing 90A (CCDC90A) 10 ug
$657.00
SC302558 CCDC90A (untagged)-Human coiled-coil domain containing 90A (CCDC90A) 10 ug
$503.00

Plasmid Map for RC203684

Download sequence for

Cloning scheme for RC203684

Circular map for RC203684

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