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C14orf169 (NM_024644) Human Tagged ORF Clone

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SKU
RC203678
C14orf169 (Myc-DDK-tagged)-Human chromosome 14 open reading frame 169 (C14orf169)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $597.00
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Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol C14orf169
Synonyms C14orf169; hsNO66; JMJD9; MAPJD; NO66; ROX; URLC2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC203678 representing NM_024644
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGATGGGCTCCAGGCCAGTGCAGGGCCGTTGAGGCGCGGGCGGCCGAAGCGCCGGCGCAAGCCCCAGC
CACACAGCGGGTCGGTCCTGGCCCTGCCCTTGAGGTCCAGGAAGATACGAAAGCAGCTGCGAAGTGTTGT
ATCCCGCATGGCAGCGCTGAGGACGCAGACGCTGCCTAGCGAGAACTCGGAGGAATCGAGGGTGGAGTCG
ACGGCCGACGACCTGGGGGACGCGCTACCCGGTGGGGCGGCGGTGGCGGCCGTCCCGGACGCAGCCCGGC
GAGAGCCATACGGCCACCTGGGGCCCGCAGAGCTGCTGGAGGCCTCGCCCGCCGCGCGCTCCCTGCAGAC
CCCGTCGGCGCGCCTGGTGCCCGCTTCCGCGCCGCCCGCGCGCCTGGTGGAGGTGCCCGCCGCGCCGGTC
CGGGTGGTGGAGACCTCGGCCCTGCTGTGCACCGCGCAACACTTAGCGGCCGTCCAGTCGTCCGGGGCCC
CTGCGACGGCGTCGGGGCCGCAGGTGGATAACACGGGTGGGGAGCCGGCCTGGGACTCCCCGCTGCGGCG
CGTCTTGGCCGAGCTGAACCGCATCCCCAGCAGCCGGCGGCGAGCGGCCCGCCTCTTTGAGTGGCTCATC
GCGCCCATGCCGCCAGATCACTTTTACCGGCGCCTATGGGAGCGCGAGGCGGTGCTGGTGCGGCGGCAGG
ACCACACCTACTACCAGGGACTTTTCTCTACCGCTGACCTGGATTCGATGCTGCGCAACGAGGAGGTGCA
GTTCGGCCAGCATTTGGACGCCGCTCGCTACATCAACGGACGACGCGAGACCCTGAACCCACCCGGCCGC
GCGCTGCCCGCCGCCGCGTGGTCCCTGTACCAGGCCGGCTGCTCCCTGCGTCTCCTCTGTCCGCAGGCTT
TCTCTACTACTGTGTGGCAGTTTTTGGCTGTGCTTCAAGAGCAGTTTGGAAGCATGGCAGGCTCCAACGT
TTACCTCACGCCCCCTAACTCGCAGGGCTTTGCCCCCCACTACGACGACATCGAGGCCTTCGTGCTGCAG
CTGGAAGGTAGGAAACTCTGGCGTGTATACCGACCCCGAGTCCCAACCGAGGAACTGGCTCTGACATCCA
GCCCCAACTTCAGTCAGGACGACCTCGGTGAGCCGGTGCTGCAGACCGTGCTGGAACCTGGAGATTTGCT
GTATTTTCCTCGGGGCTTCATTCACCAAGCTGAATGCCAGGATGGAGTCCACTCTCTGCACCTCACCTTG
TCCACGTACCAGCGCAATACCTGGGGTGACTTCTTAGAGGCCATACTGCCTCTGGCAGTGCAGGCTGCAA
TGGAAGAAAATGTGGAGTTTCGGAGGGGTCTGCCCCGAGACTTCATGGATTACATGGGGGCCCAGCATTC
AGATTCTAAGGATCCGCGAAGAACCGCTTTCATGGAGAAGGTGCGGGTCTTGGTTGCCCGCCTGGGACAC
TTTGCTCCTGTTGATGCTGTGGCCGACCAGCGAGCCAAAGACTTCATTCACGATTCTCTGCCCCCTGTTT
TGACTGATAGGGAGAGGGCACTAAGTGTTTACGGGCTTCCAATTCGCTGGGAGGCTGGAGAACCTGTAAA
CGTGGGGGCCCAGTTGACAACAGAAACAGAAGTCCATATGCTTCAGGATGGGATAGCTCGGCTGGTGGGT
GAGGGGGGCCATTTGTTTCTCTATTACACAGTGGAAAACTCCCGTGTGTATCATCTGGAAGAACCCAAGT
GCTTGGAAATATACCCCCAGCAAGCTGATGCCATGGAACTGTTGCTTGGTTCTTATCCAGAGTTTGTGAG
AGTGGGGGACCTGCCCTGTGACAGTGTGGAGGACCAGCTGTCCTTGGCAACCACGTTGTATGATAAGGGG
CTGCTGCTCACTAAGATGCCTCTAGCCCTAAAT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC203678 representing NM_024644
Red=Cloning site Green=Tags(s)

MDGLQASAGPLRRGRPKRRRKPQPHSGSVLALPLRSRKIRKQLRSVVSRMAALRTQTLPSENSEESRVES
TADDLGDALPGGAAVAAVPDAARREPYGHLGPAELLEASPAARSLQTPSARLVPASAPPARLVEVPAAPV
RVVETSALLCTAQHLAAVQSSGAPATASGPQVDNTGGEPAWDSPLRRVLAELNRIPSSRRRAARLFEWLI
APMPPDHFYRRLWEREAVLVRRQDHTYYQGLFSTADLDSMLRNEEVQFGQHLDAARYINGRRETLNPPGR
ALPAAAWSLYQAGCSLRLLCPQAFSTTVWQFLAVLQEQFGSMAGSNVYLTPPNSQGFAPHYDDIEAFVLQ
LEGRKLWRVYRPRVPTEELALTSSPNFSQDDLGEPVLQTVLEPGDLLYFPRGFIHQAECQDGVHSLHLTL
STYQRNTWGDFLEAILPLAVQAAMEENVEFRRGLPRDFMDYMGAQHSDSKDPRRTAFMEKVRVLVARLGH
FAPVDAVADQRAKDFIHDSLPPVLTDRERALSVYGLPIRWEAGEPVNVGAQLTTETEVHMLQDGIARLVG
EGGHLFLYYTVENSRVYHLEEPKCLEIYPQQADAMELLLGSYPEFVRVGDLPCDSVEDQLSLATTLYDKG
LLLTKMPLALN

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_024644
ORF Size 1923 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_024644.5
RefSeq Size 2463 bp
RefSeq ORF 1926 bp
Locus ID 79697
UniProt ID Q9H6W3
Cytogenetics 14q24.3
Protein Families Druggable Genome
MW 71.1 kDa
Summary Oxygenase that can act as both a histone lysine demethylase and a ribosomal histidine hydroxylase. Specifically demethylates 'Lys-4' (H3K4me) and 'Lys-36' (H3K36me) of histone H3, thereby playing a central role in histone code. Preferentially demethylates trimethylated H3 'Lys-4' (H3K4me3) and monomethylated H3 'Lys-4' (H3K4me1) residues, while it has weaker activity for dimethylated H3 'Lys-36' (H3K36me2). Also catalyzes the hydroxylation of 60S ribosomal protein L8 on 'His-216'. Acts as a regulator of osteoblast differentiation via its interaction with SP7/OSX by demethylating H3K4me and H3K36me, thereby inhibiting SP7/OSX-mediated promoter activation (By similarity). May also play a role in ribosome biogenesis and in the replication or remodeling of certain heterochromatic region. Participates in MYC-induced transcriptional activation.UniProtKB/Swiss-Prot Function
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"NM_024644" in other vectors (4)
SKU Description Size Price
RC203678L3 Lenti-ORF clone of C14orf169 (Myc-DDK-tagged)-Human chromosome 14 open reading frame 169 (C14orf169) 10 ug
$897.00
RC203678L4 Lenti-ORF clone of C14orf169 (mGFP-tagged)-Human chromosome 14 open reading frame 169 (C14orf169) 10 ug
$897.00
RG203678 C14orf169 (tGFP-tagged) - Human chromosome 14 open reading frame 169 (C14orf169) 10 ug
$797.00
SC122963 C14orf169 (untagged)-Human chromosome 14 open reading frame 169 (C14orf169) 10 ug
$598.00

Plasmid Map for RC203678

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC203678
Circular map for RC203678

file-alt Citations

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