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KLHDC7B (NM_138433) Human Tagged ORF Clone

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SKU
RC203654
KLHDC7B (Myc-DDK-tagged)-Human kelch domain containing 7B (KLHDC7B)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol KLHDC7B
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC203654 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCCAGGGGCCCCGCACAGCCCCCCGCGCAGAGGCCGCCTGGCCCCGCGGCCTCCTCCTCTGCGAGGC
GCTCACAGCCGGTACCCCAGCTACGGAAACGCAGCAGGTGCGAAATCGCCCCGAGCTCGGAGCAGGAGGT
CAGGCCGGCCGCCTCGGGGGACCCTCAAGGGGAGGCGCCGGGGGAGGGGGGCAGCCCTGCCGGCCGCAGC
GGGGCGCTCACGGAAAAGCAGGAGGAGGCCCGGAAGCTCATGGTGTTTCTGCAGAGGCCCGGGGGTTGGG
GGGTGGTGGAGGGGCCCCGGAAGCCCAGCTCCCGGGCCCTGGAGCCCGCCACGGCGGCAGCCCTGCGGCG
GCGGCTGGACCTGGGCAGTTGCCTGGACGTGCTGGCCTTTGCCCAGCAGCACGGAGAGCCCGGCCTGGCG
CAGGAGACCTACGCGCTGATGAGCGACAACCTGCTGCGAGTGCTGGGAGACCCGTGCCTCTACCGCCGGC
TGAGCGCGGCCGACCGCGAGCGCATCCTCAGCCTGCGGACCGGCCGGGGCCGGGCGGTGCTGGGCGTCCT
CGTACTGCCCAGCCTCTACCAGGGGGGCCGCTCAGGGCTCCCCAGGGGCCCTCGTGGCGAGGAGCCTCCT
GCGGCGGCCCCTGTGTCCCTGCCTCTACCTGCGCACCTGCATGTGTTCAACCCCCGGGAGAACACCTGGC
GGCCCCTGACCCAGGTGCCCGAGGAGGCCCCGCTTCGGGGCTGCGGTCTCTGCACCATGCACAACTACCT
GTTTCTGGCGGGGGGCATCCGTGGCTCCGGTGCCAAGGCCGTCTGCTCCAACGAGGTCTTCTGCTACAAC
CCTCTGACCAACATCTGGAGCCAGGTTCGGCCCATGCAGCAGGCCCGAGCCCAGCTCAAGCTGGTGGCCC
TGGACGGGCTGCTCTATGCCATCGGTGGCGAATGCCTGTACAGCATGGAGTGCTACGACCCGCGAACAGA
CGCCTGGACCCCACGCGCGCCACTCCCCGCAGGCACCTTCCCTGTGGCCCACGAGGCTGTGGCCTGCCGT
GGGGACATCTACGTCACCGGGGGTCACCTCTTCTACCGCCTGCTCAGGTACAGCCCCGTGAAGGATGCTT
GGGACGAGTGCCCATACAGTGCCAGCCACCGGCGTTCCAGCGACATCGTGGCACTGGGGGGCTTCCTGTA
CCGCTTCGACCTGCTGCGGGGCGTGGGCGCCGCCGTGATGCGCTACAACACAGTGACCGGCTCCTGGAGC
AGGGCTGCCTCCCTGCCCCTGCCCGCCCCCGCCCCACTGCACTGCACCACCCTGGGCAACACCATTTACT
GCCTCAACCCCCAGGTCACTGCCACCTTCACGGTCTCTGGGGGGACTGCCCAGTTCCAGGCCAAGGAGCT
GCAGCCCTTCCCCTTGGGGAGCACCGGGGTCCTCAGTCCATTCATCCTGACTCTGCCCCCTGAGGACCGG
CTGCAGACCTCACTC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC203654 protein sequence
Red=Cloning site Green=Tags(s)

MPRGPAQPPAQRPPGPAASSSARRSQPVPQLRKRSRCEIAPSSEQEVRPAASGDPQGEAPGEGGSPAGRS
GALTEKQEEARKLMVFLQRPGGWGVVEGPRKPSSRALEPATAAALRRRLDLGSCLDVLAFAQQHGEPGLA
QETYALMSDNLLRVLGDPCLYRRLSAADRERILSLRTGRGRAVLGVLVLPSLYQGGRSGLPRGPRGEEPP
AAAPVSLPLPAHLHVFNPRENTWRPLTQVPEEAPLRGCGLCTMHNYLFLAGGIRGSGAKAVCSNEVFCYN
PLTNIWSQVRPMQQARAQLKLVALDGLLYAIGGECLYSMECYDPRTDAWTPRAPLPAGTFPVAHEAVACR
GDIYVTGGHLFYRLLRYSPVKDAWDECPYSASHRRSSDIVALGGFLYRFDLLRGVGAAVMRYNTVTGSWS
RAASLPLPAPAPLHCTTLGNTIYCLNPQVTATFTVSGGTAQFQAKELQPFPLGSTGVLSPFILTLPPEDR
LQTSL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_138433
ORF Size 1485 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_138433.2, NP_612442.1
RefSeq Size 3008 bp
RefSeq ORF 3708 bp
Locus ID 113730
UniProt ID Q96G42
Cytogenetics 22q13.33
MW 53.3 kDa
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Documents
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"NM_138433" in other vectors (5)
SKU Description Size Price
RC203654L3 Lenti ORF clone of Human kelch domain containing 7B (KLHDC7B), Myc-DDK-tagged 10 ug
$757.00
RC203654L4 Lenti ORF clone of Human kelch domain containing 7B (KLHDC7B), mGFP tagged 10 ug
$757.00
RG203654 KLHDC7B (tGFP-tagged) - Human kelch domain containing 7B (KLHDC7B) 10 ug
$489.00 $657.00
SC123137 KLHDC7B (untagged)-Human kelch domain containing 7B (KLHDC7B) 10 ug
$457.00
SC327935 KLHDC7B (untagged)-Human kelch domain containing 7B (KLHDC7B) 10 ug
$609.00

Plasmid Map for RC203654

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC203654
Circular map for RC203654

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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