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NUDT8 (NM_181843) Human Tagged ORF Clone

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SKU
RC203638
NUDT8 (Myc-DDK-tagged)-Human nudix (nucleoside diphosphate linked moiety X)-type motif 8 (NUDT8)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $150.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol NUDT8
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC203638 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTGCCCGACTGCCTGTCGGCCGAGGGCGAGCTGCGCTGCCGCCGGCTGCTGGCAGGGGCCACGGCCC
GGCTCCGCGCGCGGCCCGCGTCGGCCGCGGTGCTCGTGCCGCTCTGCTCAGTGCGTGGGGTGCCGGCGCT
GCTGTACACGCTGCGGTCCAGCCGCCTGACCGGGAGGCACAAGGGCGACGTCAGTTTCCCAGGCGGCAAG
TGCGACCCGGCTGACCAAGATGTGGTGCACACGGCCCTGCGGGAAACCCGGGAGGAGCTGGGCCTGGCAG
TGCCCGAGGAGCACGTGTGGGGCCTGCTGCGGCCTGTGTATGATCCGCAAAAGGCCACCGTGGTGCCAGT
GCTTGCTGGTGTAGGCCCACTGGATCCCCAGAGCCTCAGGCCCAACTCGGAGGAGGTGAGCTGGGGGATA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC203638 protein sequence
Red=Cloning site Green=Tags(s)

MLPDCLSAEGELRCRRLLAGATARLRARPASAAVLVPLCSVRGVPALLYTLRSSRLTGRHKGDVSFPGGK
CDPADQDVVHTALRETREELGLAVPEEHVWGLLRPVYDPQKATVVPVLAGVGPLDPQSLRPNSEEVSWGI

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_181843
ORF Size 420 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_181843.1, NP_862826.1
RefSeq Size 841 bp
RefSeq ORF 423 bp
Locus ID 254552
UniProt ID Q8WV74
Cytogenetics 11q13.2
MW 15.1 kDa
Summary Probably mediates the hydrolysis of some nucleoside diphosphate derivatives.UniProtKB/Swiss-Prot Function
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"NM_181843" in other vectors (6)
SKU Description Size Price
RC203638L1 Lenti ORF clone of Human nudix (nucleoside diphosphate linked moiety X)-type motif 8 (NUDT8), Myc-DDK-tagged 10 ug
$450.00
RC203638L2 Lenti ORF clone of Human nudix (nucleoside diphosphate linked moiety X)-type motif 8 (NUDT8), mGFP tagged 10 ug
$450.00
RC203638L3 Lenti ORF clone of Human nudix (nucleoside diphosphate linked moiety X)-type motif 8 (NUDT8), Myc-DDK-tagged 10 ug
$450.00
RC203638L4 Lenti ORF clone of Human nudix (nucleoside diphosphate linked moiety X)-type motif 8 (NUDT8), mGFP tagged 10 ug
$450.00
RG203638 NUDT8 (tGFP-tagged) - Human nudix (nucleoside diphosphate linked moiety X)-type motif 8 (NUDT8) 10 ug
$350.00
SC307385 NUDT8 (untagged)-Human nudix (nucleoside diphosphate linked moiety X)-type motif 8 (NUDT8) 10 ug
$165.00

Plasmid Map for RC203638

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC203638
Circular map for RC203638

file-alt Citations

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