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NUDT6 (NM_007083) Human Tagged ORF Clone

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SKU
RC203470
NUDT6 (Myc-DDK-tagged)-Human nudix (nucleoside diphosphate linked moiety X)-type motif 6 (NUDT6), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol NUDT6
Synonyms ASFGF2; FGF-AS; FGF2AS; GFG-1; GFG1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC203470 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCGGCAGCCGCTGAGCTGGGGCCGCTGGCGCGCGATGCTTGCCCGAACCTACGGCCCCGGGCCTTCGG
CGGGTTACCGCTGGGCCTCGGGCGCACAGGGTTACGTGCGGAATCCGCCAGTTGGAGCGTGCGATCTGCA
GGGCGAGCTGGACAGATTCGGGGGCATCTCGGTGCGCCTGGCGCGGCTCGATGCGCTGGACCGCCTGGAC
GCTGCCGCCTTCCAGAAGGGCTTGCAGGCTGCAGTACAGCAATGGCGATCAGAAGGTAGAACAGCTGTAT
GGCTGCACATTCCCATCCTCCAAAGCCGATTTATTGCCCCTGCTGCTTCCCTGGGCTTCTGCTTTCACCA
CGCAGAATCGGATTCATCAACGTTGACTCTGTGGCTGAGAGAAGGGCCCAGCAGATTACCAGGATATGCT
TCACATCAAGTAGGAGTTGCAGGAGCTGTATTTGATGAAAGTACTAGAAAAATACTGGTTGTACAAGATC
GAAATAAATTGAAAAATATGTGGAAGTTTCCAGGAGGCCTGTCAGAGCCTGAAGAAGATATTGGAGACAC
AGCGGTTCGAGAAGTTTTTGAAGAGACTGGTATAAAATCAGAATTCAGGTCCGTCCTGAGTATTCGGCAA
CAGCACACAAATCCTGGAGCTTTTGGGAAGTCAGATATGTATATCATCTGCCGCCTAAAGCCATATTCAT
TCACCATAAATTTTTGCCAGGAAGAATGCTTAAGATGTGAGTGGATGGATCTCAATGACCTGGCGAAGAC
TGAAAATACAACTCCCATCACCAGCAGAGTTGCTAGGCTGCTGCTGTATGGGTACAGAGAAGGGTTTGAC
AAAATTGACCTGACTGTGGAAGAACTTCCAGCAGTTTACACAGGACTGTTTTATAAACTCTATCATAAGG
AACTGCCAGAGAATTATAAAACTATGAAAGGAATTGAT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC203470 protein sequence
Red=Cloning site Green=Tags(s)

MRQPLSWGRWRAMLARTYGPGPSAGYRWASGAQGYVRNPPVGACDLQGELDRFGGISVRLARLDALDRLD
AAAFQKGLQAAVQQWRSEGRTAVWLHIPILQSRFIAPAASLGFCFHHAESDSSTLTLWLREGPSRLPGYA
SHQVGVAGAVFDESTRKILVVQDRNKLKNMWKFPGGLSEPEEDIGDTAVREVFEETGIKSEFRSVLSIRQ
QHTNPGAFGKSDMYIICRLKPYSFTINFCQEECLRCEWMDLNDLAKTENTTPITSRVARLLLYGYREGFD
KIDLTVEELPAVYTGLFYKLYHKELPENYKTMKGID

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_007083
ORF Size 948 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_007083.2
RefSeq Size 1197 bp
RefSeq ORF 951 bp
Locus ID 11162
UniProt ID P53370
Cytogenetics 4q28.1
Domains NUDIX
MW 35.7 kDa
Summary This gene overlaps and lies on the opposite strand from FGF2 gene, and is thought to be the FGF2 antisense gene. The two genes are independently transcribed, and their expression shows an inverse relationship, suggesting that this antisense transcript may regulate FGF2 expression. This gene has also been shown to have hormone-regulatory and antiproliferative actions in the pituitary that are independent of FGF2 expression. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Oct 2011
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"NM_007083" in other vectors (6)
SKU Description Size Price
RC203470L1 Lenti ORF clone of Human nudix (nucleoside diphosphate linked moiety X)-type motif 6 (NUDT6), transcript variant 1, Myc-DDK-tagged 10 ug
$600.00
RC203470L2 Lenti ORF clone of Human nudix (nucleoside diphosphate linked moiety X)-type motif 6 (NUDT6), transcript variant 1, mGFP tagged 10 ug
$600.00
RC203470L3 Lenti ORF clone of Human nudix (nucleoside diphosphate linked moiety X)-type motif 6 (NUDT6), transcript variant 1, Myc-DDK-tagged 10 ug
$600.00
RC203470L4 Lenti ORF clone of Human nudix (nucleoside diphosphate linked moiety X)-type motif 6 (NUDT6), transcript variant 1, mGFP tagged 10 ug
$600.00
RG203470 NUDT6 (tGFP-tagged) - Human nudix (nucleoside diphosphate linked moiety X)-type motif 6 (NUDT6), transcript variant 1 10 ug
$489.00 $500.00
SC315525 NUDT6 (untagged)-Human nudix (nucleoside diphosphate linked moiety X)-type motif 6 (NUDT6), transcript variant 1 10 ug
$330.00

Plasmid Map for RC203470

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC203470
Circular map for RC203470

file-alt Citations

The involvement of non-coding RNAs in smooth muscle cell dynamics in atherosclerosis and abdominal aortic aneurysm
Winter
Thesis (2023)
Snippet: EV plasmid PS100001 Origene (Rockville, MD, USA) GFP plasmid gifted Dr. Francesca Fasolo DH5a competent cells 18263-012 Sigma Aldrich (St.Louis, MO, USA) NUDT6 plasmid RC203470 Origene (Rockville, MD, USA) NUDT6 sirna 122202 Thermo Fisher Scientific
Showing 1-1 of 1 papers.
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