ALS2CR2 (STRADB) (NM_018571) Human Tagged ORF Clone

SKU RC203432

STRADB (Myc-DDK-tagged)-Human STE20-related kinase adaptor beta (STRADB), transcript variant 1

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: ALS2CR2
• Synonyms: ALS2CR2; CALS-21; ILPIP; ILPIPA; PAPK; PRO1038
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203432 ORF sequence; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGTCTCTTTTGGATTGCTTCTGCACTTCAAGAACACAAGTTGAATCACTCAGACCTGAAAAACAGTCTG; AAACCAGTATCCATCAATACTTGGTTGATGAGCCAACCCTTTCCTGGTCACGTCCATCCACTAGAGCCAG; TGAAGTACTATGTTCCACCAACGTTTCTCACTATGAGCTCCAAGTAGAAATAGGAAGAGGATTTGACAAC; TTGACTTCTGTCCATCTTGCACGGCATACTCCCACGGGAACACTGGTAACTATAAAAATTACAAATCTGG; AAAACTGCAATGAAGAACGCCTGAAAGCTTTACAGAAAGCCGTGATTCTATCCCACTTTTTCCGGCATCC; CAATATTACAACTTATTGGACAGTTTTCACTGTTGGCAGCTGGCTTTGGGTTATTTCTCCATTTATGGCC; TATGGTTCAGCAAGTCAACTCTTGAGGACCTATTTTCCTGAAGGAATGAGTGAAACTTTAATAAGAAACA; TTCTCTTTGGAGCCGTGAGAGGGTTGAACTATCTGCACCAAAATGGCTGTATTCACAGGAGTATTAAAGC; CAGCCATATCCTCATTTCTGGTGATGGCCTAGTGACCCTCTCTGGCCTTTCCCATCTGCATAGTTTGGTT; AAGCATGGACAGAGGCATAGGGCTGTGTATGATTTCCCACAGTTCAGCACATCAGTGCAGCCGTGGCTGA; GTCCAGAACTACTGAGACAGGATTTACATGGGTATAATGTGAAGTCAGATATTTACAGTGTTGGGATTAC; AGCATGTGAATTAGCCAGTGGGCAGGTGCCTTTCCAGGACATGCATAGAACTCAGATGCTGTTACAGAAA; CTGAAAGGTCCTCCTTATAGCCCATTGGATATCAGTATTTTCCCTCAATCAGAATCCAGAATGAAAAATT; CCCAGTCAGGTGTAGACTCTGGGATTGGAGAAAGTGTGCTTGTCTCCAGTGGAACTCACACAGTAAATAG; TGACCGATTACACACACCATCCTCAAAAACTTTCTCTCCTGCCTTCTTTAGCTTGGTACAGCTCTGTTTG; CAACAAGATCCTGAGAAAAGGCCATCAGCAAGCAGTTTATTGTCCCATGTTTTCTTCAAACAGATGAAAG; AAGAAAGCCAGGATTCAATACTTTCACTGTTGCCTCCTGCTTATAACAAGCCATCAATATCATTGCCTCC; AGTGTTACCTTGGACTGAGCCAGAATGTGATTTTCCTGATGAAAAAGACTCATACTGGGAATTC; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203432 protein sequence; Red=Cloning site Green=Tags(s); ; MSLLDCFCTSRTQVESLRPEKQSETSIHQYLVDEPTLSWSRPSTRASEVLCSTNVSHYELQVEIGRGFDN; LTSVHLARHTPTGTLVTIKITNLENCNEERLKALQKAVILSHFFRHPNITTYWTVFTVGSWLWVISPFMA; YGSASQLLRTYFPEGMSETLIRNILFGAVRGLNYLHQNGCIHRSIKASHILISGDGLVTLSGLSHLHSLV; KHGQRHRAVYDFPQFSTSVQPWLSPELLRQDLHGYNVKSDIYSVGITACELASGQVPFQDMHRTQMLLQK; LKGPPYSPLDISIFPQSESRMKNSQSGVDSGIGESVLVSSGTHTVNSDRLHTPSSKTFSPAFFSLVQLCL; QQDPEKRPSASSLLSHVFFKQMKEESQDSILSLLPPAYNKPSISLPPVLPWTEPECDFPDEKDSYWEF; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018571
• ORF Size: 1254 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_018571.3
• RefSeq Size: 2300 bp
• RefSeq ORF: 1257 bp
• Locus ID: 55437
• UniProt ID: Q9C0K7
• Cytogenetics: 2q33.1
• Domains: pkinase, S_TKc, TyrKc
• Protein Families: Druggable Genome, Protein Kinase
• MW: 47 kDa
• Summary: This gene encodes a protein that belongs to the serine/threonine protein kinase STE20 subfamily. One of the active site residues in the protein kinase domain of this protein is altered, and it is thus a pseudokinase. This protein is a component of a complex involved in the activation of serine/threonine kinase 11, a master kinase that regulates cell polarity and energy-generating metabolism. This complex regulates the relocation of this kinase from the nucleus to the cytoplasm, and it is essential for G1 cell cycle arrest mediated by this kinase. The protein encoded by this gene can also interact with the X chromosome-linked inhibitor of apoptosis protein, and this interaction enhances the anti-apoptotic activity of this protein via the JNK1 signal transduction pathway. Two pseudogenes, located on chromosomes 1 and 7, have been found for this gene. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2011]