TOMM22 (NM_020243) Human Tagged ORF Clone

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SKU
RC203351
TOMM22 (Myc-DDK-tagged)-Human translocase of outer mitochondrial membrane 22 homolog (yeast) (TOMM22), nuclear gene encoding mitochondrial protein
  $150.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol TOMM22
Synonyms 1C9-2; MST065; MSTP065; TOM22
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC203351 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTGCCGCCGTCGCTGCTGCCGGTGCAGGGGAACCCCAGTCCCCGGACGAATTGCTCCCGAAAGGCG
ACGCGGAGAAGCCTGAGGAGGAGCTGGAGGAGGACGACGATGAGGAGCTAGATGAGACCCTGTCGGAGAG
ACTATGGGGCCTGACGGAGATGTTTCCGGAGAGGGTCCGGTCCGCGGCCGGAGCCACTTTTGATCTTTCC
CTCTTTGTGGCTCAGAAAATGTACAGGTTTTCCAGGGCAGCCTTGTGGATTGGGACCACTTCCTTTATGA
TCCTGGTTCTTCCCGTTGTCTTTGAGACGGAGAAGTTGCAAATGGAGCAACAGCAGCAACTGCAGCAGCG
GCAGATACTTCTAGGACCTAACACAGGGCTCTCAGGAGGAATGCCAGGGGCTCTACCCTCACTTCCTGGA
AAGATC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC203351 protein sequence
Red=Cloning site Green=Tags(s)

MAAAVAAAGAGEPQSPDELLPKGDAEKPEEELEEDDDEELDETLSERLWGLTEMFPERVRSAAGATFDLS
LFVAQKMYRFSRAALWIGTTSFMILVLPVVFETEKLQMEQQQQLQQRQILLGPNTGLSGGMPGALPSLPG
KI

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_020243
ORF Size 426 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_020243.5
RefSeq Size 1396 bp
RefSeq ORF 429 bp
Locus ID 56993
UniProt ID Q9NS69, Q549C5
Cytogenetics 22q13.1
Protein Families Druggable Genome, Transmembrane
MW 15.5 kDa
Summary The protein encoded by this gene is an integral membrane protein of the mitochondrial outer membrane. The encoded protein interacts with TOMM20 and TOMM40, and forms a complex with several other proteins to import cytosolic preproteins into the mitochondrion. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_020243" in other vectors (6)
SKU Description Size Price
RC203351L1 Lenti ORF clone of Human translocase of outer mitochondrial membrane 22 homolog (yeast) (TOMM22), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged 10 ug
$450.00
RC203351L2 Lenti ORF clone of Human translocase of outer mitochondrial membrane 22 homolog (yeast) (TOMM22), nuclear gene encoding mitochondrial protein, mGFP tagged 10 ug
$450.00
RC203351L3 Lenti ORF clone of Human translocase of outer mitochondrial membrane 22 homolog (yeast) (TOMM22), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged 10 ug
$450.00
RC203351L4 Lenti ORF clone of Human translocase of outer mitochondrial membrane 22 homolog (yeast) (TOMM22), nuclear gene encoding mitochondrial protein, mGFP tagged 10 ug
$450.00
RG203351 TOMM22 (tGFP-tagged) - Human translocase of outer mitochondrial membrane 22 homolog (yeast) (TOMM22), nuclear gene encoding mitochondrial protein 10 ug
$350.00
SC113156 TOMM22 (untagged)-Human translocase of outer mitochondrial membrane 22 homolog (yeast) (TOMM22), nuclear gene encoding mitochondrial protein 10 ug
$150.00

Plasmid Map for RC203351

Download sequence for

Cloning scheme for RC203351

Circular map for RC203351

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