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Fc epsilon RI (FCER1A) (NM_002001) Human Tagged ORF Clone

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SKU
RC203321
FCER1A (Myc-DDK-tagged)-Human Fc fragment of IgE, high affinity I, receptor for, alpha polypeptide (FCER1A)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol Fc epsilon RI
Synonyms FCE1A; FcERI
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC203321 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTCCTGCCATGGAATCCCCTACTCTACTGTGTGTAGCCTTACTGTTCTTCGCTCCAGATGGCGTGT
TAGCAGTCCCTCAGAAACCTAAGGTCTCCTTGAACCCTCCATGGAATAGAATATTTAAAGGAGAGAATGT
GACTCTTACATGTAATGGGAACAATTTCTTTGAAGTCAGTTCCACCAAATGGTTCCACAATGGCAGCCTT
TCAGAAGAGACAAATTCAAGTTTGAATATTGTGAATGCCAAATTTGAAGACAGTGGAGAATACAAATGTC
AGCACCAACAAGTTAATGAGAGTGAACCTGTGTACCTGGAAGTCTTCAGTGACTGGCTGCTCCTTCAGGC
CTCTGCTGAGGTGGTGATGGAGGGCCAGCCCCTCTTCCTCAGGTGCCATGGTTGGAGGAACTGGGATGTG
TACAAGGTGATCTATTATAAGGATGGTGAAGCTCTCAAGTACTGGTATGAGAACCACAACATCTCCATTA
CAAATGCCACAGTTGAAGACAGTGGAACCTACTACTGTACGGGCAAAGTGTGGCAGCTGGACTATGAGTC
TGAGCCCCTCAACATTACTGTAATAAAAGCTCCGCGTGAGAAGTACTGGCTACAATTTTTTATCCCATTG
TTGGTGGTGATTCTGTTTGCTGTGGACACAGGATTATTTATCTCAACTCAGCAGCAGGCCACATTTCTCT
TGAAGATTAAGAGAACCAGGAAAGGCTTCAGACTTCTGAACCCACATCCTAAGCCAAACCCCAAAAACAA
C

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC203321 protein sequence
Red=Cloning site Green=Tags(s)

MAPAMESPTLLCVALLFFAPDGVLAVPQKPKVSLNPPWNRIFKGENVTLTCNGNNFFEVSSTKWFHNGSL
SEETNSSLNIVNAKFEDSGEYKCQHQQVNESEPVYLEVFSDWLLLQASAEVVMEGQPLFLRCHGWRNWDV
YKVIYYKDGEALKYWYENHNISITNATVEDSGTYYCTGKVWQLDYESEPLNITVIKAPREKYWLQFFIPL
LVVILFAVDTGLFISTQQQATFLLKIKRTRKGFRLLNPHPKPNPKNN

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_002001
ORF Size 771 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_002001.3
RefSeq Size 1198 bp
RefSeq ORF 774 bp
Locus ID 2205
UniProt ID P12319
Cytogenetics 1q23.2
Protein Families Transmembrane
Protein Pathways Asthma, Fc epsilon RI signaling pathway
MW 29.6 kDa
Summary The immunoglobulin epsilon receptor (IgE receptor) is the initiator of the allergic response. When two or more high-affinity IgE receptors are brought together by allergen-bound IgE molecules, mediators such as histamine that are responsible for allergy symptoms are released. This receptor is comprised of an alpha subunit, a beta subunit, and two gamma subunits. The protein encoded by this gene represents the alpha subunit. provided by RefSeq, Aug 2011
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"NM_002001" in other vectors (6)
SKU Description Size Price
RC203321L1 Lenti ORF clone of Human Fc fragment of IgE, high affinity I, receptor for; alpha polypeptide (FCER1A), Myc-DDK-tagged 10 ug
$600.00
RC203321L2 Lenti ORF clone of Human Fc fragment of IgE, high affinity I, receptor for; alpha polypeptide (FCER1A), mGFP tagged 10 ug
$600.00
RC203321L3 Lenti ORF clone of Human Fc fragment of IgE, high affinity I, receptor for; alpha polypeptide (FCER1A), Myc-DDK-tagged 10 ug
$600.00
RC203321L4 Lenti ORF clone of Human Fc fragment of IgE, high affinity I, receptor for; alpha polypeptide (FCER1A), mGFP tagged 10 ug
$600.00
RG203321 FCER1A (tGFP-tagged) - Human Fc fragment of IgE, high affinity I, receptor for; alpha polypeptide (FCER1A) 10 ug
$500.00
SC122614 FCER1A (untagged)-Human Fc fragment of IgE, high affinity I, receptor for, alpha polypeptide (FCER1A) 10 ug
$300.00

Plasmid Map for RC203321

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC203321
Circular map for RC203321

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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