MGST3 (NM_004528) Human Tagged ORF Clone

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SKU
RC203290
MGST3 (Myc-DDK-tagged)-Human microsomal glutathione S-transferase 3 (MGST3)
  $150.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol MGST3
Synonyms GST-III
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC203290 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTGTCCTCTCTAAGGAATATGGTTTTGTGCTTCTAACTGGTGCTGCCAGCTTTATAATGGTGGCCC
ACCTAGCCATCAATGTTTCCAAGGCCCGCAAGAAGTACAAAGTGGAGTATCCTATCATGTACAGCACGGA
CCCTGAAAATGGGCACATCTTCAACTGCATTCAGCGAGCCCACCAGAACACGTTGGAAGTGTATCCTCCC
TTCTTATTTTTTCTAGCTGTTGGAGGTGTTTACCACCCGCGTATAGCTTCTGGCCTGGGCTTGGCCTGGA
TTGTTGGACGAGTTCTTTATGCTTACGGCTATTACACGGGAGAACCCAGCAAGCGTAGTCGAGGAGCCCT
GGGGTCCATCGCCCTCCTGGGCTTGGTGGGCACAACTGTGTGCTCTGCTTTCCAGCATCTTGGTTGGGTT
AAAAGTGGCTTGGGCAGTGGACCCAAATGCTGCCAT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC203290 protein sequence
Red=Cloning site Green=Tags(s)

MAVLSKEYGFVLLTGAASFIMVAHLAINVSKARKKYKVEYPIMYSTDPENGHIFNCIQRAHQNTLEVYPP
FLFFLAVGGVYHPRIASGLGLAWIVGRVLYAYGYYTGEPSKRSRGALGSIALLGLVGTTVCSAFQHLGWV
KSGLGSGPKCCH

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_004528
ORF Size 456 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_004528.4
RefSeq Size 1528 bp
RefSeq ORF 459 bp
Locus ID 4259
UniProt ID O14880, A0A024R8Z1
Cytogenetics 1q24.1
Domains MAPEG
Protein Families Druggable Genome, Transmembrane
Protein Pathways Drug metabolism - cytochrome P450, Glutathione metabolism, Metabolism of xenobiotics by cytochrome P450
MW 16.5 kDa
Summary This gene encodes a member of the MAPEG (Membrane Associated Proteins in Eicosanoid and Glutathione metabolism) protein family. Members of this family are involved in the production of leukotrienes and prostaglandin E, important mediators of inflammation. This gene encodes an enzyme which catalyzes the conjugation of leukotriene A4 and reduced glutathione to produce leukotriene C4. This enzyme also demonstrates glutathione-dependent peroxidase activity towards lipid hydroperoxides.provided by RefSeq, May 2011
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Documents
Resources
"NM_004528" in other vectors (4)
SKU Description Size Price
RC203290L3 Lenti ORF clone of Human microsomal glutathione S-transferase 3 (MGST3), Myc-DDK-tagged 10 ug
$450.00
RC203290L4 Lenti ORF clone of Human microsomal glutathione S-transferase 3 (MGST3), mGFP tagged 10 ug
$450.00
RG203290 MGST3 (tGFP-tagged) - Human microsomal glutathione S-transferase 3 (MGST3) 10 ug
$489.00
SC108230 MGST3 (untagged)-Human microsomal glutathione S-transferase 3 (MGST3) 10 ug
$150.00

Plasmid Map for RC203290

Download sequence for

Cloning scheme for RC203290

Circular map for RC203290

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