Density Regulated Protein (DENR) (NM_003677) Human Tagged ORF Clone
$300.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | Density Regulated Protein |
| Synonyms | DRP; DRP1; SMAP-3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_003677 |
| ORF Size | 594 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_003677.5 |
| RefSeq Size | 3061 bp |
| RefSeq ORF | 597 bp |
| Locus ID | 8562 |
| UniProt ID | O43583 |
| Cytogenetics | 12q24.31 |
| MW | 22.1 kDa |
| Summary | This gene encodes a protein whose expression was found to increase in cultured cells at high density but not during growth arrest. This gene was also shown to have increased expression in cells overexpressing HER-2/neu proto-oncogene. The protein contains an SUI1 domain. In budding yeast, SUI1 is a translation initiation factor that along with eIF-2 and the initiator tRNA-Met, directs the ribosome to the proper translation start site. Proteins similar to SUI have been found in mammals, insects, and plants. provided by RefSeq, Jul 2008 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_003677" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC203227L3 | Lenti ORF clone of Human density-regulated protein (DENR), Myc-DDK-tagged | 10 ug |
$600.00
|
|
| RC203227L4 | Lenti ORF clone of Human density-regulated protein (DENR), mGFP tagged | 10 ug |
$600.00
|
|
| RG203227 | DENR (tGFP-tagged) - Human density-regulated protein (DENR) | 10 ug |
$489.00
$500.00
|
|
| SC321520 | DENR (untagged)-Human density-regulated protein (DENR) | 10 ug |
$300.00
|
|
Citations
The intrinsically disordered region of eIF5B stimulates IRES usage and nucleates biological granule formation
Harris, Marr
Cell Rep (2023) 42 (10), 113283 DOI: 10.1016/j.celrep.2023.113283
Harris, Marr
Cell Rep (2023) 42 (10), 113283 DOI: 10.1016/j.celrep.2023.113283
Cited by 8
AI Summary: OriGene products contributed to this study by providing the DENR (Myc-DDK-tagged)-Human density-regulated protein expression vector (RC203227) and the EIF2A (Myc-DDK-tagged)-Human eukaryotic translation initiation factor 2A expression vector (RC204303), which were used to investigate the roles of these proteins in IRES-mediated translation and stress response mechanisms.
Snippet: ic_BB-CBh-hSpCas9 Feng Zhang Addgene plasmid # 42230 pJDS246 expressing cas9 Keith Joung Addgene plasmid # 43861 pGL3 Promega Empty firefly luciferase vector RC203227 Origene DENR (Myc-DDK-tagged)-Human density-regulated protein (DENR) expression vector RC204303 Origene EIF2A (Myc-DDK-tagged)-Human eukaryotic translation initiation factor 2A, 65kDa (EI
Mitochondrial Fission and Mitophagy Coordinately Restrict High Glucose Toxicity in Cardiomyocytes
Kobayashi, Zhao, Zhang et al
Front Physiol (2020) 11, 604069 DOI: 10.3389/fphys.2020.604069
Kobayashi, Zhao, Zhang et al
Front Physiol (2020) 11, 604069 DOI: 10.3389/fphys.2020.604069
Cited by 53
AI Summary: OriGene’s human DRP1 and Parkin cDNA clones were used in this study to manipulate mitochondrial fission and mitophagy pathways, enabling the authors to investigate their roles in protecting cardiomyocytes from high glucose-induced toxicity.
Snippet: to all media. Replication-Deficient Adenoviruses. We purchased the human DRP1 and Parkin cDNA clones from OriGene and obtained the mitophagy reporter mt-Rosella from Dr. Devenish (Mijaljica et al., 2011). Rosella is a
Doxorubicin-induced cardiomyocyte death is mediated by unchecked mitochondrial fission and mitophagy
Catanzaro, Weiner, Kaminaris et al
FASEB J. (2019)
Catanzaro, Weiner, Kaminaris et al
FASEB J. (2019)
AI Summary: OriGene contributed to this study by providing the human DRP1 cDNA clone, which was used to investigate the role of DRP1 in doxorubicin-induced mitochondrial fragmentation and cardiomyocyte death.
Snippet: Anti-phospho-PDHE1-A type I (Ser293) antibody was purchased from MilliporeSigma (ABS204). Replication-deficient adenoviruses The human DRP1 cDNA clone was obtained from OriGene Technologies (Rockville, MD, USA)
The Fine Tuning of Drp1-Dependent Mitochondrial Remodeling and Autophagy Controls Neuronal Differentiation
Vantaggiato, Castelli, Giovarelli et al
Front Cell Neurosci (2019) 13, 120 DOI: 10.3389/fncel.2019.00120
Vantaggiato, Castelli, Giovarelli et al
Front Cell Neurosci (2019) 13, 120 DOI: 10.3389/fncel.2019.00120
AI Summary: OriGene products, specifically the pCMV6-MycDDK-Drp1wt and pCMV6-MycDDK-Drp1K38A expression vectors, were used to manipulate Drp1 levels in order to study its role in mitochondrial remodeling and neuronal differentiation.
Snippet: pCMV6-MycDDK-Drp1wt and pCMV6-MycDDK-Drp1K38A vectors were purchased from OriGene Technologies, Inc., Rockville, MD, USA. The plasmids for RNA interference studies were purchased from SABiosciences Corporation (Frederick, MD, USA)
Showing 1-4 of 4 papers.
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