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TMEM126A (NM_032273) Human Tagged ORF Clone

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SKU
RC203188
TMEM126A (Myc-DDK-tagged)-Human transmembrane protein 126A (TMEM126A)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol TMEM126A
Synonyms OPA7
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC203188 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAAAATCATAAATCCAATAATAAGGAAAACATAACAATTGTTGATATATCCAGAAAAATTAACCAGC
TTCCAGAAGCAGAAAGGAATCTACTTGAAAATGGATCGGTTTATGTTGGATTAAATGCTGCTCTTTGTGG
CCTCATAGCAAACAGTCTTTTTCGACGCATCTTGAATGTGACAAAGGCTCGCATAGCTGCTGGCTTACCA
ATGGCAGGGATACCTTTTCTTACAACAGACTTAACTTACAGATGTTTTGTAAGTTTTCCTTTGAATACAG
GTGATTTGGATTGTGAAACCTGTACCATAACACGGAGTGGACTGACTGGTCTTGTTATTGGTGGTCTATA
CCCTGTTTTCTTGGCTATACCTGTAAATGGTGGTCTAGCAGCCAGGTATCAATCAGCTCTGTTACCACAC
AAAGGGAACATCTTAAGTTACTGGATTAGAACTTCTAAGCCTGTCTTTAGAAAGATGTTATTTCCTATTT
TGCTCCAGACTATGTTTTCAGCATACCTTGGGTCTGAACAATATAAACTACTTATAAAGGCCCTTCAGTT
ATCTGAACCTGGCAAAGAAATTCAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC203188 protein sequence
Red=Cloning site Green=Tags(s)

MENHKSNNKENITIVDISRKINQLPEAERNLLENGSVYVGLNAALCGLIANSLFRRILNVTKARIAAGLP
MAGIPFLTTDLTYRCFVSFPLNTGDLDCETCTITRSGLTGLVIGGLYPVFLAIPVNGGLAARYQSALLPH
KGNILSYWIRTSKPVFRKMLFPILLQTMFSAYLGSEQYKLLIKALQLSEPGKEIH

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_032273
ORF Size 585 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_032273.4
RefSeq Size 818 bp
RefSeq ORF 588 bp
Locus ID 84233
UniProt ID Q9H061
Cytogenetics 11q14.1
Protein Families Transmembrane
MW 21.5 kDa
Summary The protein encoded by this gene is a mitochondrial membrane protein of unknown function. Defects in this gene are a cause of optic atrophy type 7 (OPA7). Two transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Sep 2011
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"NM_032273" in other vectors (4)
SKU Description Size Price
RC203188L3 Lenti ORF clone of Human transmembrane protein 126A (TMEM126A), Myc-DDK-tagged 10 ug
$600.00
RC203188L4 Lenti ORF clone of Human transmembrane protein 126A (TMEM126A), mGFP tagged 10 ug
$600.00
RG203188 TMEM126A (tGFP-tagged) - Human transmembrane protein 126A (TMEM126A) 10 ug
$489.00 $500.00
SC123019 TMEM126A (untagged)-Human transmembrane protein 126A (TMEM126A) 10 ug
$300.00

Plasmid Map for RC203188

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC203188
Circular map for RC203188

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