SRP19 (NM_003135) Human Tagged ORF Clone

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SKU
RC203129
SRP19 (Myc-DDK-tagged)-Human signal recognition particle 19kDa (SRP19), transcript variant 1
  $150.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol SRP19
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC203129 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTTGCACTGCCGCGCGGTCCCCGGCCGACCAGGACAGGTTTATTTGTATCTATCCTGCTTATTTAA
ATAATAAGAAGACCATCGCAGAGGGAAGGCGAATCCCCATAAGTAAGGCTGTTGAAAATCCTACAGCTAC
AGAGATTCAAGATGTATGTTCAGCAGTTGGACTTAACGTATTTCTTGAGAAAAATAAAATGTACTCTAGA
GAATGGAATCGTGATGTCCAATACAGAGGCAGAGTCCGGGTCCAGCTCAAACAGGAAGATGGGAGCCTCT
GCCTTGTACAGTTCCCATCACGTAAGTCAGTAATGTTGTATGCAGCAGAAATGATACCTAAACTAAAAAC
AAGGACACAAAAAACAGGAGGTGCTGACCAAAGTCTTCAACAAGGAGAGGGAAGTAAAAAAGGGAAAGGA
AAGAAAAAGAAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC203129 protein sequence
Red=Cloning site Green=Tags(s)

MACTAARSPADQDRFICIYPAYLNNKKTIAEGRRIPISKAVENPTATEIQDVCSAVGLNVFLEKNKMYSR
EWNRDVQYRGRVRVQLKQEDGSLCLVQFPSRKSVMLYAAEMIPKLKTRTQKTGGADQSLQQGEGSKKGKG
KKKK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_003135
ORF Size 432 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_003135.3
RefSeq Size 1502 bp
RefSeq ORF 435 bp
Locus ID 6728
UniProt ID P09132
Cytogenetics 5q22.2
Domains SRP19
Protein Pathways Protein export
MW 16.2 kDa
Summary Signal-recognition-particle assembly, binds directly to 7S RNA and mediates binding of the 54 kDa subunit of the SRP.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_003135" in other vectors (6)
SKU Description Size Price
RC203129L1 Lenti ORF clone of Human signal recognition particle 19kDa (SRP19), transcript variant 1, Myc-DDK-tagged 10 ug
$450.00
RC203129L2 Lenti ORF clone of Human signal recognition particle 19kDa (SRP19), transcript variant 1, mGFP tagged 10 ug
$450.00
RC203129L3 Lenti ORF clone of Human signal recognition particle 19kDa (SRP19), transcript variant 1, Myc-DDK-tagged 10 ug
$450.00
RC203129L4 Lenti ORF clone of Human signal recognition particle 19kDa (SRP19), transcript variant 1, mGFP tagged 10 ug
$450.00
RG203129 SRP19 (tGFP-tagged) - Human signal recognition particle 19kDa (SRP19), transcript variant 1 10 ug
$489.00
SC118181 SRP19 (untagged)-Human signal recognition particle 19kDa (SRP19), transcript variant 1 10 ug
$150.00

Plasmid Map for RC203129

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Cloning scheme for RC203129

Circular map for RC203129

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