IEX1 (IER3) (NM_003897) Human Tagged ORF Clone

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SKU
RC202979
IER3 (Myc-DDK-tagged)-Human immediate early response 3 (IER3)
  $225.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol IEX1
Synonyms DIF-2; DIF2; GLY96; IEX-1; IEX-1L; IEX1; PRG1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC202979 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTGTCACTCTCGCAGCTGCCACCCGACCATGACCATCCTGCAGGCCCCGACCCCGGCCCCCTCCACCA
TCCCGGGACCCCGGCGGGGCTCCGGTCCTGAGATCTTCACCTTCGACCCTCTCCCGGAGCCCGCAGCGGC
CCCTGCCGGGCGCCCCAGCGCCTCTCGCGGGCACCGAAAGCGCAGCCGCAGGGTTCTCTACCCTCGAGTG
GTCCGGCGCCAGCTGCCAGTCGAGGAACCGAACCCAGCCAAAAGGCTTCTCTTTCTGCTGCTCACCATCG
TCTTCTGCCAGATCCTGATGGCTGAAGAGGGTGTGCCGGCGCCCCTGCCTCCAGAGGACGCCCCTAACGC
CGCATCCCTGGCGCCCACCCCTGTGTCCCCCGTCCTCGAGCCCTTTAATCTGACTTCGGAGCCCTCGGAC
TACGCTCTGGACCTCAGCACTTTCCTCCAGCAACACCCGGCCGCCTTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC202979 protein sequence
Red=Cloning site Green=Tags(s)

MCHSRSCHPTMTILQAPTPAPSTIPGPRRGSGPEIFTFDPLPEPAAAPAGRPSASRGHRKRSRRVLYPRV
VRRQLPVEEPNPAKRLLFLLLTIVFCQILMAEEGVPAPLPPEDAPNAASLAPTPVSPVLEPFNLTSEPSD
YALDLSTFLQQHPAAF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_003897
ORF Size 468 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_003897.2, NP_003888.1
RefSeq Size 1254 bp
RefSeq ORF 471 bp
Locus ID 8870
UniProt ID P46695, A0A1U9X7X2
Cytogenetics 6p21.33
Protein Families Druggable Genome
MW 16.9 kDa
Summary This gene functions in the protection of cells from Fas- or tumor necrosis factor type alpha-induced apoptosis. Partially degraded and unspliced transcripts are found after virus infection in vitro, but these transcripts are not found in vivo and do not generate a valid protein. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_003897" in other vectors (4)
SKU Description Size Price
RC202979L3 Lenti-ORF clone of IER3 (Myc-DDK-tagged)-Human immediate early response 3 (IER3) 10 ug
$525.00
RC202979L4 Lenti-ORF clone of IER3 (mGFP-tagged)-Human immediate early response 3 (IER3) 10 ug
$525.00
RG202979 IER3 (tGFP-tagged) - Human immediate early response 3 (IER3) 10 ug
$425.00
SC110006 IER3 (untagged)-Human immediate early response 3 (IER3) 10 ug
$225.00

Plasmid Map for RC202979

Download sequence for

Cloning scheme for RC202979

Circular map for RC202979

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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