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Syntaxin 6 (STX6) (NM_005819) Human Tagged ORF Clone

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SKU
RC202951
STX6 (Myc-DDK-tagged)-Human syntaxin 6 (STX6)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol Syntaxin 6
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC202951 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCCATGGAGGACCCCTTCTTTGTGGTGAAAGGAGAGGTACAGAAAGCAGTCAACACTGCCCAGGGAT
TGTTTCAGAGATGGACAGAGCTCCTCCAGGACCCCTCCACAGCAACAAGGGAAGAAATCGACTGGACCAC
CAACGAGCTGAGAAATAACCTCCGGAGCATAGAGTGGGATCTAGAGGACCTTGATGAAACCATCAGCATA
GTTGAAGCAAATCCTAGAAAATTTAACCTTGATGCAACTGAATTGAGTATAAGAAAAGCCTTCATTACAA
GTACTCGGCAAGTTGTCAGGGACATGAAAGATCAGATGTCAACTTCATCTGTGCAGGCATTAGCTGAAAG
AAAAAATAGACAGGCACTGCTGGGAGACAGTGGCAGCCAGAACTGGAGCACTGGAACAACAGATAAATAT
GGGCGTCTGGACCGAGAGCTCCAGAGAGCCAATTCTCATTTCATTGAGGAGCAGCAGGCACAGCAGCAGT
TGATCGTGGAACAGCAGGATGAGCAGTTGGAGCTGGTCTCTGGCAGCATCGGGGTGCTGAAGAACATGTC
CCAGCGCATCGGAGGGGAGCTGGAGGAACAGGCAGTTATGTTGGAAGATTTCTCTCACGAATTGGAGAGC
ACTCAGTCCCGGCTGGACAATGTGATGAAGAAACTTGCAAAAGTATCTCATATGACCAGTGATCGGCGCC
AATGGTGTGCCATAGCCATCCTCTTTGCAGTCCTGTTGGTTGTGCTCATCCTCTTCTTAGTGCTG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC202951 protein sequence
Red=Cloning site Green=Tags(s)

MSMEDPFFVVKGEVQKAVNTAQGLFQRWTELLQDPSTATREEIDWTTNELRNNLRSIEWDLEDLDETISI
VEANPRKFNLDATELSIRKAFITSTRQVVRDMKDQMSTSSVQALAERKNRQALLGDSGSQNWSTGTTDKY
GRLDRELQRANSHFIEEQQAQQQLIVEQQDEQLELVSGSIGVLKNMSQRIGGELEEQAVMLEDFSHELES
TQSRLDNVMKKLAKVSHMTSDRRQWCAIAILFAVLLVVLILFLVL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_005819
ORF Size 765 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_005819.6
RefSeq Size 5074 bp
RefSeq ORF 768 bp
Locus ID 10228
UniProt ID O43752
Cytogenetics 1q25.3
Domains t_SNARE
Protein Families Druggable Genome, Transmembrane
Protein Pathways SNARE interactions in vesicular transport
MW 29.2 kDa
Summary Involved in intracellular vesicle trafficking.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_005819" in other vectors (6)
SKU Description Size Price
RC202951L1 Lenti ORF clone of Human syntaxin 6 (STX6), Myc-DDK-tagged 10 ug
$600.00
RC202951L2 Lenti ORF clone of Human syntaxin 6 (STX6), mGFP tagged 10 ug
$600.00
RC202951L3 Lenti ORF clone of Human syntaxin 6 (STX6), Myc-DDK-tagged 10 ug
$600.00
RC202951L4 Lenti ORF clone of Human syntaxin 6 (STX6), mGFP tagged 10 ug
$600.00
RG202951 STX6 (tGFP-tagged) - Human syntaxin 6 (STX6) 10 ug
$489.00 $500.00
SC108182 STX6 (untagged)-Human syntaxin 6 (STX6) 10 ug
$300.00

Plasmid Map for RC202951

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC202951
Circular map for RC202951

file-alt Citations

The iRhom homology domain is indispensable for ADAM17-mediated TNFα and EGF receptor ligand release
Düsterhöft, Kahveci-Türköz, Wozniak et al
Cell Mol Life Sci (2021)
Cited by 25
AI Summary: OriGene’s human STX6 cDNA clone (RC202951) was used for cell transfection experiments to investigate the role of vesicle transport proteins in the trafficking and function of the iRhom-ADAM17 complex.
Snippet: When indicated cells were transfected with either human STX6 (RC202951;OriGene Technologies),
Showing 1-1 of 1 papers.
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